We present the results of an association study involving hospitalized coronavirus disease 2019 (COVID-19) patients with a clinical background during the 3rd pandemic wave of COVID-19 in Slovakia. Seventeen single nucleotide variants (SNVs) in the eleven most relevant genes, according to the COVID-19 Host Genetics Initiative, were investigated. Our study confirms the validity of the influence of LZTFL1 and 2'-5'-oligoadenylate synthetase (OAS)1/OAS3 genetic variants on the severity of COVID-19. For two LZTFL1 SNVs in complete linkage disequilibrium, rs17713054 and rs73064425, the odds ratios of baseline allelic associations and logistic regressions (LR) adjusted for age and sex ranged in the four tested designs from 2.04 to 2.41 and from 2.05 to 3.98, respectively. The OAS1/OAS3 haplotype 'gttg' carrying a functional allele G of splice-acceptor variant rs10774671 manifested its protective function in the Delta pandemic wave. Significant baseline allelic associations of two DPP9 variants in all tested designs and two IFNAR2 variants in the Omicron pandemic wave were not confirmed by adjusted LR. Nevertheless, adjusted LR showed significant associations of NOTCH4 rs3131294 and TYK2 rs2304256 variants with severity of COVID-19. Hospitalized patients' reported comorbidities were not correlated with genetic variants, except for obesity, smoking (IFNAR2), and hypertension (NOTCH4). The results of our study suggest that host genetic variations have an impact on the severity and duration of acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Considering the differences in allelic associations between pandemic waves, they support the hypothesis that every new SARS-CoV-2 variant may modify the host immune response by reconfiguring involved pathways.
- MeSH
- 2',5'-Oligoadenylate Synthetase genetics MeSH
- COVID-19 * genetics epidemiology virology MeSH
- Adult MeSH
- Genetic Predisposition to Disease MeSH
- Polymorphism, Single Nucleotide * MeSH
- Cohort Studies MeSH
- Middle Aged MeSH
- Humans MeSH
- SARS-CoV-2 * genetics MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Slovakia MeSH
BACKGROUND: Prostate cancer (PCa) is the second most commonly diagnosed cancer in men. To date, the role of the combined application of long non-coding RNAs (PCA3, DLX1, HOXC6, TMPRSS2:ERG) for obtaining the most accurate method of detection of PCa has not yet been comprehensively investigated. METHODS: In total 240 persons were included in the retrospective study. Among them were 150 patients with confirmed PCa, 30 patients with benign prostatic hyperplasia, 30 patients with active chronic prostatitis and 30 healthy volunteers. In all patients, the urine samples were collected prior to biopsy or treatment. Polymerase chain reaction with reverse transcription was performed to detect the expression level of PCA3, HOXC6, DLX1 and the presence of the TMPRSS2:ERG transcript. RESULTS: PCA3 was detected in urine samples in all cases. Using a PCA3 score of 56 allowed the differentiation between PCa and all other cases with a sensitivity of 61% and specificity of 96% (p < 0.001) while a PCA3 score threshold value of 50 resulted in a differentiation between clinically significant PCa (ISUP grades 2-5) and all other cases with a sensitivity of 93% and specificity of 93% (p < 0.001). The TMPRSS2:ERG expression in urine was detected exclusively in the group of patients with PCa and only in 16% of all cases. CONCLUSIONS: PCA3 score detected in urine demonstrated moderate sensitivity and good specificity in differentiation between PCa and non-PCa and high sensitivity and specificity in differentiation between clinically significant PCa and non-PCa.
- MeSH
- Antigens, Neoplasm * genetics MeSH
- Oncogene Proteins, Fusion genetics urine MeSH
- Homeodomain Proteins genetics MeSH
- Humans MeSH
- Biomarkers, Tumor urine MeSH
- Prostatic Neoplasms * diagnosis genetics MeSH
- Prostate pathology MeSH
- Prostate-Specific Antigen MeSH
- Retrospective Studies MeSH
- Serine Endopeptidases genetics MeSH
- Transcriptional Regulator ERG MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
Prvé vydanie 136 stran : ilustrace, tabulky ; 24 cm
Publikácia sa zameriava na komunikáciu medzi fyzioterapeutom a pacientom. Určené odbornej verejnosti.
Cieľ: Cieľom práce bolo poukázať na dôležitosť vykonávania pohybovej aktivity, dodržiavanie režimových opatrení vo vzťahu ku kvalite života u pacientov po infarkte myokardu (IM). Metódy a metodika: Sledovanú skupinu tvorilo 77 pacientov s prekonaným akútnym IM (66,2 % mužov a 33,8 % žien) s priemerným vekom 64,8 rokov (43–86 rokov). Zber dát bol pomocou anonymného štandardizovaného dotazníku MacNew QLMI (Quality of Life after Myocardial Infarction), zameraného na hodnotenie kvality života, doplneného demografickými otázkami, ako aj otázkami zameranými na dodržiavanie režimových opatrení a zmenu životného štýlu. Výsledky: Napriek tomu, že takmer všetci probandi deklarovali, že boli po prekonaní IM oboznámení o nutnosti režimových opatrení, len časť probandov ich dostatočne ovláda (55,8 %) a ešte menšia časť uplatňuje v praxi (35,1 %). Odporúčanie dodržiavajú viac mladší pacienti, častejšie ženy ako muži (so štatistickou významnosťou rozdielov). S dodržiavaním režimových opatrení priamoúmerne súvisí kvalita života pacientov po infarkte. Záver: Zmena životného štýlu po IM (ako súčasť sekundárnej prevencie) zlepšuje nielen prognózu, ale aj kvalitu života. Nie celkom uspokojivá aplikácia do praxe môže mať subjektívne príčiny (absencia motivácie u pacienta), ako aj nedostatočne intenzívna edukácia zo strany zdravotníckeho personálu.
Aim: The aim of the work was to point out the importance of physical activity and adherence to regimen measures in relation to the quality of life in patients after myocardial infarction (MI). Methodology: The study group consisted of 77 patients with an acute MI (66.2% men and 33.8% women) with a mean age of 64.8 years (from 43 to 86 years). The data collection was performed using the anonymous standardised MacNew QLMI questionnaire, aimed at assessing quality of life, supplemented by demographic issues as well as questions aimed at changing and adherence to lifestyle. Results: Despite the fact that almost all probands declared that they were informed about the need for regimen measures after overcoming an MI, only a part of the probands sufficiently control them (55.8 %) and an even smaller part applies them in practice (35.1 %). Younger patients are more likely to follow the recommendations, women more often than men (with statistical significance of differences). Adherence to regimen measures is directly related to the quality of life of patients after a heart attack. Conclusion: Lifestyle change after an MI (as part of secondary prevention) improves not only the prognosis but also the quality of life. Less than satisfactory application in practice may have subjective causes (absence of motivation in the patient), as well as insufficiently intensive education by nursing staff.
- Keywords
- režimová opatření,
- MeSH
- Myocardial Infarction * MeSH
- Quality of Life MeSH
- Humans MeSH
- Motor Activity MeSH
- Surveys and Questionnaires MeSH
- Life Style * MeSH
- Check Tag
- Humans MeSH
V príspevku upozorníme na rizikové faktory (RF) kardiovaskulárnych ochorení (KVO) u žien po menopauze, pretože aj pri dobrej informovanosti o prevencii KVO sledujeme nárast fajčenia aj v mladších vekových skupinách, vzostup prevalencie hypertenzie a obezity, zvýšenie koncentrácie celkového cholesterolu a nedostatok telesnej aktivity. Podľa výsledkov nášho výskumu bolo najviac respondentiek zaradených do kategórie rizikovej hodnoty WHR (68 %), až 50 % respondentiek spadá do kategórie „obezita“ a podľa obvodu pása je 75 % žien po menopauze v najrizikovejšej skupine.
- Keywords
- prevence,
- MeSH
- Cardiovascular Diseases MeSH
- Menopause MeSH
- Risk Factors MeSH
- Research MeSH
