- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Fifty-five strains of enterococci isolated from the piglet intestine were characterized in vitro for probiotic activity. Identification of the isolates revealed Enterococcus faecium as the predominant species (84 %). Forty strains (73 %) were found to produce bacteriocin-like substances (only into solid media) with activity almost only toward Gram-positive genera. Thirty-eight % of strains were resistant to tetracycline, 27 % to chloramphenicol, 18 % to erythromycin and 16 % to vancomycin. In addition to control of strain safety, 6 % of isolates were beta-hemolytic and 16 % produced gelatinase. Seven strains selected for further probiotic assays exhibited sufficient survival rate at pH 3.0 after 3 h, in the presence of 1 % ox-bile and lysozyme after 1 d (over 107 CFU/mL in all tests). The adhesion of tested strains to porcine and human intestinal mucus was found in a similar range (1.4-14.0 % and 1.4-17.6 %, respectively). In accordance with current research effort to use and/or to combine various health promoting substances, the sensitivity of all isolates toward plant extracts and toward bacteriocins produced by animal and environmental strains was determined. All enterococci were sensitive toward oregano and sage extracts and toward one (E. faecium EF55--chicken isolate, activity of 25 600 AU/mL) of ten bacteriocin substances. It means that a similar anti-enterococcal potential of some bacteriocin substances may be observed as for certain plant extracts.
- MeSH
- antibakteriální látky farmakologie MeSH
- antibióza MeSH
- bakteriální adheze MeSH
- bakteriální léková rezistence MeSH
- bakteriociny biosyntéza farmakologie MeSH
- dobromysl (rod) chemie MeSH
- Enterococcus MeSH
- financování organizované MeSH
- gastrointestinální trakt mikrobiologie MeSH
- kyseliny toxicita MeSH
- mikrobiální testy citlivosti MeSH
- mikrobiální viabilita MeSH
- muciny metabolismus MeSH
- muramidasa MeSH
- prasata mikrobiologie MeSH
- probiotika farmakologie MeSH
- rostlinné extrakty farmakologie MeSH
- žlučové kyseliny a soli toxicita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
The effect of daily supplementation of nisin (2 mg/L), monensin (5.88 mg/L) and nisin and monensin (2 + 5.88 mg/L) on ovine ruminal ciliates and bacteria was investigated using the artificial rumen RUSITEC. Major groups in RUSITEC were Entodinium spp. and Dasytricha ruminantium. The supplementation of nisin significantly increased the population of both major ciliate groups. The supplementation of monensin significantly decreased the population of both groups. The combined effect of nisin and monensin was similar to the effect of monensin. Monensin had strong antiprotozoic effects in contrast to the stimulatory effects of nisin. D. ruminantium followed by Entodinium spp. appeared more resistant to tested compounds than other rumen ciliates. Tested additives did not significantly influence the presence and growth of amylolytic streptococci and enterococci but nisin showed a tendency to decreasing the concentration of Escherichia coli and lactobacilli.
- MeSH
- bachor mikrobiologie parazitologie MeSH
- Bacteria účinky léků MeSH
- dezinficiencia aplikace a dávkování MeSH
- dieta metody MeSH
- eukaryotické buňky účinky léků MeSH
- financování organizované MeSH
- monensin aplikace a dávkování MeSH
- nisin aplikace a dávkování MeSH
- ovce MeSH
- teoretické modely MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
