Genes encoding glycosyl hydrolase family 11 (GH11) xylanases and xylanases have been identified from Pseudobutyrivibrio xylanivorans. In contrast, little is known about the diversity and distribution of the GH10 xylanase in strains of P. xylanivorans. Xylanase and associated activities of P. xylanivorans have been characterized in detail in the type strain, Mz5. The aim of the present study was to identify GH10 xylanase genes in strains 2 and Mz5 of P. xylanivorans. In addition, we evaluated degradation and utilization of xylan by P. xylanivorans 2 isolated from rumen of Creole goats. After a 12-h culture, P. xylanivorans 2 was able to utilize up to 53% of the total pentose content present in birchwood xylan (BWX) and to utilize up to 62% of a ethanol-acetic acid-soluble fraction prepared from BWX. This is the first report describing the presence of GH10 xylanase-encoding genes in P. xylanivorans. Strain 2 and Mz5 contained xylanases which were related to GH10 xylanase of Butyrivibrio sp. Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries.
- MeSH
- bachor mikrobiologie MeSH
- Bacteria klasifikace enzymologie genetika izolace a purifikace MeSH
- bakteriální proteiny chemie genetika metabolismus MeSH
- endo-1,4-beta-xylanasy chemie genetika metabolismus MeSH
- fylogeneze MeSH
- kinetika MeSH
- kozy MeSH
- xylany metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Pseudobutyrivibrio xylanivorans strain Mz5(T), an anaerobic bacterium (originating from the rumen of a Holstein-Friesian cow), has some attributes that make it a possible probiotic strain (very active hydrolases, bacteriocin and conjugated linoleic acid production). For the estimation of its adhesion ability, the adhesion test on Caco-2 cells was introduced and adapted. The adhesion was performed in an anaerobic glove box in standard 24-well plates at neutral pH for 30 min. The best method for separation of the adhered bacteria from Caco-2 cells appeared to be homogenization with an automatic pipette. The number of adhered bacteria was too small to be determined microscopically, so a new approach, i.e. detection of the apparent lag phase in liquid growth medium was tested. Under the selected assay conditions 1.04 bacterial cells from the late exponential phase adhered to one Caco-2 cell, which confirms the adhesion capability of P. xylanivorans Mz5(T). The adapted adhesion test using Caco-2 cells is suitable for estimation of adhesion capability of anaerobic bacteria.
- MeSH
- anaerobní bakterie izolace a purifikace růst a vývoj MeSH
- bachor mikrobiologie MeSH
- bakteriální adheze fyziologie genetika imunologie MeSH
- Caco-2 buňky enzymologie mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- kyselina máselná izolace a purifikace metabolismus MeSH
- kyselina mléčná izolace a purifikace metabolismus MeSH
- Lactobacillus metabolismus růst a vývoj MeSH
- probiotika izolace a purifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- Caco-2 buňky enzymologie mikrobiologie sekrece MeSH
- elektrická impedance klasifikace MeSH
- ELISA metody využití MeSH
- enterocyty fyziologie MeSH
- financování vládou MeSH
- interleukin-8 škodlivé účinky MeSH
- Lactobacillus enzymologie metabolismus MeSH
- lidé MeSH
- Salmonella enteritidis enzymologie metabolismus MeSH
- Check Tag
- lidé MeSH
- MeSH
- bachor fyziologie mikrobiologie MeSH
- Bacteria enzymologie genetika metabolismus MeSH
- bakteriální geny genetika MeSH
- financování vládou MeSH
- otevřené čtecí rámce genetika MeSH
- polymerázová řetězová reakce metody využití MeSH
- sekvenční analýza MeSH
- xylany metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
