- MeSH
- kosti a kostní tkáň abnormality MeSH
- nemoci ryb vrozené diagnostické zobrazování MeSH
- ploutve zvířat abnormality MeSH
- prevalence MeSH
- rybníky MeSH
- Salmonidae abnormality anatomie a histologie MeSH
- vodní hospodářství metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
The hop metabolome important for the brewing industry and for medical purposes is endangered worldwide due to multiple viroid infections affecting hop physiology. Combinatorial biolistic hop inoculation with Citrus bark cracking viroid (CBCVd), Apple fruit crinkle viroid (AFCVd), Hop latent viroid, and Hop stunt viroid (HSVd) showed a low CBCVd compatibility with HSVd, while all other viroid combinations were highly compatible. Unlike to other viroids, single CBCVd propagation showed a significant excess of (-) over (+) strands in hop, tomato, and Nicotiana benthamiana, but not in citruses. Inoculation of hop with all viroids led to multiple infections with unstable viroid levels in individual plants in the pre- and post-dormancy periods, and to high plant mortality and morphological disorders. Hop isolates of CBCVd and AFCVd were highly stable, only minor quasispecies were detected. CBCVd caused a strong suppression of some crucial mRNAs related to the hop prenylflavonoid biosynthesis pathway, while AFCVd-caused effects were moderate. According to mRNA degradome analysis, this suppression was not caused by a direct viroid-specific small RNA-mediated degradation. CBCVd infection led to a strong induction of two hop transcription factors from WRKY family and to a disbalance of WRKY/WDR1 complexes important for activation of lupulin genes.
- MeSH
- Citrus genetika virologie MeSH
- Humulus genetika virologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- Malus genetika virologie MeSH
- messenger RNA genetika MeSH
- ovoce genetika virologie MeSH
- tabák genetika virologie MeSH
- viroidy genetika patogenita MeSH
- Publikační typ
- časopisecké články MeSH
Nuclease from tomato (TBN1) was produced by in planta biotechnology purified and tested for its anticarcinogenic properties. The nuclease was cytostatic after its intratumoral administration to nude mice bearing human melanoma or prostate carcinoma or after tumor targeting by TBN1 administration intravenously as conjugate with polyethylene glycol (PEG). Inhibitory effects of TBN1 on tumor growth were comparable to effects of bovine seminal RNase (BS-RNase), but the inhibition was reached at about ten times lower protein concentration. Simultaneously, TBN1 exhibited a lower degree of embryotoxicity compared to BS-RNAse and other nucleases. TBN1 showed significant stability in vivo, because it was readily detected after its administration intratumorally or intravenously by the fluorescence methods. Intravenous administration of TBN1-PEG caused significant inhibition of tumor proliferation without obvious degenerative changes, while direct administration of TBN1 into melanoma tumors led to rapid tumor tissue degeneration. The fact can be essential for the mode of TBN1 biological action that mature nuclease is a small (36 kDa) thermostable glycoprotein that has ability to destroy human 28S, 18S, 7S and 5.8S RNA, circular RNAs, double-stranded RNA in vitro and shows DNase and 3'nucleotidase activities.
- MeSH
- antitumorózní látky farmakologie MeSH
- apoptóza účinky léků MeSH
- dvouvláknová RNA genetika metabolismus MeSH
- embryo savčí enzymologie patologie MeSH
- endodeoxyribonukleasy farmakologie MeSH
- fluorescenční protilátková technika MeSH
- glykosylace MeSH
- lidé MeSH
- melanom experimentální patologie MeSH
- míra přežití MeSH
- myši nahé MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- nádory prostaty patologie MeSH
- rekombinantní proteiny terapeutické užití MeSH
- skot MeSH
- Solanum lycopersicum enzymologie MeSH
- testikulární nádory patologie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- acyltransferasy genetika chemie MeSH
- chalkonoidy analýza MeSH
- financování organizované MeSH
- fylogeneze MeSH
- Humulus enzymologie chemie MeSH
- klonování DNA MeSH
- messenger RNA analýza MeSH
- prenylace proteinů MeSH
- promotorové oblasti (genetika) MeSH
- protoonkogenní proteiny c-myb genetika chemie MeSH
- rostlinné proteiny genetika chemie MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- MeSH
- antitumorózní látky farmakologie terapeutické užití MeSH
- apoptóza účinky záření MeSH
- finanční podpora výzkumu jako téma MeSH
- lidé MeSH
- nádory terapie MeSH
- proteiny buněčného cyklu terapeutické užití MeSH
- ribonukleasy imunologie terapeutické užití MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- MeSH
- antitumorózní látky aplikace a dávkování imunologie MeSH
- buněčné dělení účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- neuroblastom farmakoterapie MeSH
- pankreas enzymologie MeSH
- ribonukleasy aplikace a dávkování farmakologie MeSH
- skot MeSH
- spermie enzymologie MeSH
- transplantace nádorů MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
