The impact of four pre-treatment techniques on the surface morphology and chemistry, residual stress, mechanical properties, corrosion resistance in a physiological saline solution and cell colonization of commercially pure titanium is examined in detail. Mechanical polishing, electrochemical etching, chemical etching in Kroll's reagent, and ion sputter etching with argon ions were applied. Surface morphologies reflect the nature of surface layer removal. Significant roughening of the surface and a characteristic microtopology become apparent as a result of the sensitivity of chemical and ion sputter etching to the grain orientation. The hardness in the near surface region was controlled by the amount of residual stress. Etching of the stressed surface layer led to a reduction in residual stress and surface hardness. A compact passivation layer composed of TiO, TiO2 and Ti2O3 native oxides imparted high corrosion resistance to the surface after mechanical polishing, chemical and electrochemical etching. The ion sputter etched surface showed substantially reduced corrosion resistance, where the corrosion process was controlled by electron transfer. The specific topology affected the adhesion of the cell to the surface rather than the cell area coverage. The cell area coverage increased with the corrosion stability of the surface.
- MeSH
- buněčné linie MeSH
- elektrochemické techniky MeSH
- koroze MeSH
- lidé MeSH
- oxidy chemie MeSH
- povrchové vlastnosti MeSH
- testování materiálů MeSH
- titan chemie MeSH
- tvrdost MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Titanium dioxide (TiO2) and Ag-incorporated diamond-like carbon (DLC) films were prepared on different substrates. The films were prepared by pulsed laser deposition (PLD). TiO2 and Ag were selected due to their potential values as biomaterials. Silver is effective against a wide range of spectrum including Gram-negative and Gram-positive bacteria and yeast. TiO2 and Ag-incorporated DLC thin films are suitable candidates for application on biomedical devices and implants due to their biocompatibility, chemical inertness, and mechanical properties. Thin films are widely used in coronary artery stents, dental implants, heart valves and other vascular devices. The microstructure and antibacterial properties of TiO2 and silver-doped diamond-like carbon (DLC) films have been investigated. The films structural quality was evaluated using SEM microscopy, AFM microscopy and Raman spectroscopy. The antibacterial activity was determined using Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus subtilis. Our results demonstrate that the TiO2, nitrogen doped titanium oxides TON and Ag-incorporated DLC films are potentially useful as biomedical materials having good antibacterial properties.
Diamond-like carbon (DLC) thin films are frequently used for coating of implants. The problem of DLC layers lies in bad layer adhesion to metal implants. Chromium is used as a dopant for improvement of adhesion of DLC films. DLC and Cr-DLC layers were deposited on silicon, Ti6Al4V and CoCrMo substrates by a hybrid technology using combination of pulsed laser deposition (PLD) and magnetron sputtering. The topology of layers was studied using SEM, AFM and mechanical profilometer. Carbon and chromium content and concentration of trivalent and toxic hexavalent chromium bonds were determined by XPS and WDS. It follows from the scratch tests that Cr doping improved adhesion of DLC layers. Ethylene glycol, diiodomethane and deionized water were used to measure the contact angles. The surface free energy (SFE) was calculated. The antibacterial properties were studied using Pseudomonas aeruginosa and Staphylococcus aureus bacteria. The influence of SFE, hydrophobicity and surface roughness on antibacterial ability of doped layers is discussed.
- Klíčová slova
- UV-C záření,
- MeSH
- lasery excimerové normy terapeutické užití využití MeSH
- lidé MeSH
- nehty patologie MeSH
- onychomykóza patofyziologie terapie MeSH
- techniky in vitro MeSH
- ultrafialové záření MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- hodnotící studie MeSH
The possibilities of using newly developed nano- and micro-thin films in biomedicine are intensively studied at the present time. Many research institutions are looking for ways to evaluate mechanical properties of these films. One of the most important and frequently studied characteristics is practical adhesion. A very important method for evaluating the practical adhesion strength is scratch test. Often, however, the research teams use a method based on the disunity evaluation of adhesion of biocompatible surface layer. This makes the quantitative comparison of research results impossible. We designed and tested new evaluation method and procedure based on international standards in order to eliminate these problems. This article is aimed at showing the new possibility of using established standards for evaluating adhesion of nano- and micro-thin biocompatible films and at showing the application of the standards to evaluate the often studied DLC biocompatible layers. The thickness of the film was 470 nm. As a substrate a titanium alloy Ti6Al4V was used.
- MeSH
- adhezivita MeSH
- akustika MeSH
- biokompatibilní materiály chemie MeSH
- biokompatibilní potahované materiály chemie MeSH
- diamant chemie MeSH
- internacionalita MeSH
- mechanický stres MeSH
- nanočástice chemie MeSH
- pevnost v tahu MeSH
- referenční standardy MeSH
- testování materiálů metody normy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
