The increased interest in assisted reproduction through in vitro fertilization (IVF) leads to an urgent need to identify biomarkers that reliably highly predict the success of pregnancy. Despite advances in diagnostics, treatment, and IVF approaches, the 30% success rate of IVF seems insurmountable. Idiopathic infertility does not have any explanation for IVF failure especially when a patient is treated with a healthy competitive embryo capable of implantation and development. Since appropriate intercellular communication is essential after embryo implantation, the emergence of the investigation of embryonic secretome including short non-coding RNA (sncRNA) molecules is crucial. That's why biomarker identification, sncRNAs secreted during the IVF process into the blastocyst's cultivation medium, by the implementation of artificial intelligence opens the door to a better understanding of the bidirectional communication between embryonic cells and the endometrium and so the success of the IVF. This study presents a set of promising new sncRNAs which are revealed to predictively distinguish a high-quality embryo, suitable for an embryo transfer in the IVF process, from a low-quality embryo with 86% accuracy. The identified exact combination of miRNAs/piRNAs as a non-invasively obtained biomarker for quality embryo determination, increasing the likelihood of implantation and the success of pregnancy after an embryo transfer.
Cardiovascular comorbidities are independent risk factors for mortality in dialysis patients. MicroRNA signaling has an important role in vascular aging and cardiac health, while physical activity is a primary nonpharmacologic treatment for cardiovascular comorbidities in dialysis patients. To identify the relationships between muscle function, miRNA signaling pathways, the presence of vascular calcifications and the severity of cardiovascular comorbidities, we initially enrolled 90 subjects on hemodialysis therapy and collected complete data from 46 subjects. A group of 26 subjects inactiv group (INC) was monitored during 12 weeks of physical inactivity and another group of 20 patients exercise group (EXC) was followed during 12 weeks of intradialytic, moderate intensity, resistance training intervention applied three times per week. In both groups, we assessed the expression levels of myo-miRNAs, proteins, and muscle function (MF) before and after the 12-week period. Data on the presence of vascular calcifications and the severity of cardiac comorbidities were collected from the patients' EuCliD® records. Using a full structural equitation modelling of the total study sample, we found that the higher the increase in MF was observed in patients, the higher the probability of a decrease in the expression of miR-206 and TRIM63 and the lower severity of cardiac comorbidities. A reduced structural model in INC patients showed that the higher the decrease in MF, the higher the probability of the presence of calcifications and the higher severity of cardiac comorbidities. In EXC patients, we found that the higher the increase in MF, the lower the probability of higher severity of cardiovascular comorbidities.
- MeSH
- cévní endotel metabolismus MeSH
- cvičení fyziologie MeSH
- dialýza ledvin * MeSH
- kardiovaskulární nemoci krev genetika terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA biosyntéza krev genetika MeSH
- sedavý životní styl MeSH
- senioři MeSH
- stanovení celkové genové exprese metody MeSH
- stárnutí krev genetika MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
The miRNA-206 and miRNA-23a play an important role in muscle tissue hypertrophy, regeneration and atrophy. Both of these miRNAs have been highlighted as promising adaptation predictors; however, the available evidence on associations is inconclusive. Therefore, our aim was to assess the expression levels of these two miRNAs as predictors of change in muscle function during strength training and physical inactivity among dialysed patients. For this purpose, 46 haemodialysis patients were monitored for 12-weeks of either intradialytic strength training (EXG, n = 20) or physical inactivity during dialysis (CON, n = 26). In both groups of patients, we assessed the baseline expression levels of miRNA-23a and miRNA-206 and the isometric force generated during hip flexion (HF) contraction before and after the 12-week period. Among the EXG group, the expression of miRNA-206 predicted the change in HF (R2 = 0.63, p = 0.0005) much more strongly than the expression of miRNA-23a (R2 = 0.21, p = 0.027). Interestingly, baseline miRNA-23a (R2 = 0.30, p = 0.006) predicted the change in HF much more than miRNA-206 (p = ns) among the CON group. Our study indicates that the baseline expression of miRNA-206 could predict the response to strength training, while miRNA-23a could serve as a potential predictive marker of functional changes during physical inactivity in dialysis patients.
- MeSH
- biologické markery analýza MeSH
- dialýza ledvin metody MeSH
- fyziologická adaptace MeSH
- kondiční příprava zvířat * MeSH
- kosterní svaly metabolismus patofyziologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA analýza genetika MeSH
- odporový trénink * MeSH
- sedavý životní styl * MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Intradialytic resistance training (IRT) protects patients' muscle mass and functions against protein-energy wasting, malnutrition and cachexia. However, the evidence of the effects of such an intervention in haemodialysis patients is limited and not conclusive. To improve the applicability of such interventions, we need a better understanding of molecular, functional and psycho-social adaptation in dialysed patients following a physical training. Therefore, the aim of this study is to investigate the effects of IRT on lower extremity muscle functions, quality of life, and anxiety and depression, clinical outcomes and circulatory micro-ribonucleic acid (miRNA) profiles in patients on chronic haemodialysis therapy. METHODS: We will perform a quasi-experimental study in 3 dialysis centres. Patients will be recruited via their nephrologists and will be allocated to an experimental and a control group based on the location of the patients' dialysis centre. Patients allocated to the experimental group will undergo a 12-week IRT, while the control group will remain physically inactive during dialysis. The primary outcome is the change in the maximal force produced during an isometric contraction of lower extremity muscles. Secondary outcomes regard quality of life, anxiety and depression, clinical outcomes and circulatory miRNA profiles. Patients' level of health literacy defined as the ability to get and understand health information will be also measured in the study as a potential modifier of effects. DISCUSSION: This quasi-experimental study can add in an important way to our understanding of the effects of resistance training on dialysis patients' muscle strength, quality of life and disease-specific outcomes.
- MeSH
- chronické selhání ledvin patofyziologie psychologie terapie MeSH
- deprese terapie MeSH
- dialýza ledvin * MeSH
- isometrická kontrakce MeSH
- klinická studie jako téma * MeSH
- kvalita života MeSH
- lidé MeSH
- mikro RNA krev MeSH
- multicentrické studie jako téma MeSH
- odporový trénink * metody MeSH
- svalová síla MeSH
- úzkost terapie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- protokol klinické studie MeSH
Úvod: Biochemické zmeny sprevádzajúce intrakapsulárne patologické procesy predstavujú potenciálny zdroj informácií využiteľný na diagnostiku, diferenciálnu diagnostiku či monitorovanie ochorení temporomandibulárneho kĺbu. Miniinvazívne zásahy do integrity temporomandibulárneho kĺbu so sprístupnením synoviálnej tekutiny sa v stomatologickej praxi realizujú za účelom drenáže, prípadne terapeutických laváží a nie za účelom stanovenia akéhokoľvek biochemického parametra. Okrem invazivity punkcie temporomandibulárneho kĺbu využiteľnosť synoviálnej tekutiny z tejto lokality na diagnostiku komplikuje jej nízky objem. Cieľom práce je zhrnúť aktuálne poznatky o histológii, fyziológii a patofyziológii temporomandibulárneho kĺbu, vyhodnotiť možnosti vyšetrenia synoviálnej tekutiny a otestovať diagnostický potenciál tejto tekutiny pomocou vysoko citlivej fluorescenčnej fingerprintovej analýzy.
Introduction: Biochemical changes accompanying intracapsular pathological processes represent potential source of information exploitable in diagnostics, differential diagnostics or monitoring of temporomandibular joint disorders. Miniinvasive intervensions in the temporomandibular joint integrity with synovial fluid acces are performed in dental practice performed for drainage or therapeutic lavage and not for determination of any biochemical parameter. In addition to the invasiveness of the temporomandibular joint puncture, the synovial fluid utilization from this site for diagnostics is complicated by low volume. Aim of this work is to summarize current knowledge of histology, physiology and pathophysiology of the temporomandibular joint, to evaluate the possibilities of synovial fluid (ST) examination and to test the diagnostic potential of this fluid by means of highly sensitive fluorescence fingerprint analysis.
- MeSH
- artrocentéza metody MeSH
- fluorescence MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- nemoci temporomandibulárního kloubu * diagnóza patofyziologie MeSH
- osteoartróza * diagnóza MeSH
- synoviální tekutina * MeSH
- temporomandibulární kloub * patofyziologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
