The colonization, translocation and protective effect of two intestinal bacteria - PR4 (pig commensal strain of Bifidobacterium choerinum) or EcN (probiotic Escherichia coli strain Nissle 1917) - against subsequent infection with a virulent LT2 strain of Salmonella enterica serovar Typhimurium were studied in gnotobiotic pigs after oral association. The clinical state of experimental animals correlated with bacterial translocation and levels of inflammatory cytokines [a chemokine, interleukin (IL)-8, a proinflammatory cytokine, tumour necrosis factor (TNF)-α and an anti-inflammatory cytokine, IL-10] in plasma and intestinal lavages. Gnotobiotic pigs orally mono-associated with either PR4 or EcN thrived, and bacteria were not found in their blood. No significant inflammatory cytokine response was observed. Mono-association with Salmonella caused devastating septicaemia characterized by high levels of IL-10 and TNF-α in plasma and TNF-α in the intestine. Di-associated gnotobiotic pigs were given PR4 or EcN for 24 h. Subsequently, they were infected orally with Salmonella and euthanized 24 h later. Pigs associated with bifidobacteria before Salmonella infection suffered from severe systemic infection and mounted similar cytokine responses as pigs infected with Salmonella alone. In contrast, EcN interfered with translocation of Salmonella into mesenteric lymph nodes and systemic circulation. Pigs pre-associated with EcN thrived and their clinical condition correlated with the absence of IL-10 in their plasma and a decrease of TNF-α in plasma and ileum.
- MeSH
- antibióza MeSH
- Bifidobacterium imunologie MeSH
- cytokiny analýza krev MeSH
- Escherichia coli imunologie MeSH
- gnotobiologické modely MeSH
- ileum imunologie mikrobiologie MeSH
- kolon imunologie mikrobiologie MeSH
- prasata MeSH
- probiotika terapeutické užití MeSH
- Salmonella typhimurium imunologie MeSH
- salmonelová infekce u zvířat imunologie terapie MeSH
- střeva imunologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- MUTAFLOR,
- MeSH
- epitelové buňky MeSH
- Escherichia coli MeSH
- exprese genu MeSH
- probiotika farmakologie MeSH
- střeva MeSH
- Publikační typ
- kongresy MeSH
- Klíčová slova
- MUTAFLOR,
- MeSH
- Escherichia coli MeSH
- feces analýza MeSH
- polymerázová řetězová reakce MeSH
- probiotika farmakologie MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- dítě MeSH
- Escherichia coli imunologie klasifikace růst a vývoj MeSH
- infekce spojené se zdravotní péčí imunologie MeSH
- infekce vyvolané Escherichia coli imunologie prevence a kontrola MeSH
- lidé MeSH
- nemoci nedonošenců prevence a kontrola MeSH
- novorozenec MeSH
- probiotika terapeutické užití MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- přehledy MeSH
