- MeSH
- Coloring Agents MeSH
- Eosinophils cytology pathology MeSH
- Cats * MeSH
- Mastocytosis * surgery pathology veterinary MeSH
- Mast Cells cytology pathology MeSH
- Stomach Diseases * surgery pathology veterinary MeSH
- Tolonium Chloride MeSH
- Gastric Mucosa cytology pathology MeSH
- Animals MeSH
- Check Tag
- Cats * MeSH
- Animals MeSH
- Publication type
- Case Reports MeSH
Chromatin compaction mediates progenitor to post-mitotic cell transitions and modulates gene expression programs, yet the mechanisms are poorly defined. Snf2h and Snf2l are ATP-dependent chromatin remodelling proteins that assemble, reposition and space nucleosomes, and are robustly expressed in the brain. Here we show that mice conditionally inactivated for Snf2h in neural progenitors have reduced levels of histone H1 and H2A variants that compromise chromatin fluidity and transcriptional programs within the developing cerebellum. Disorganized chromatin limits Purkinje and granule neuron progenitor expansion, resulting in abnormal post-natal foliation, while deregulated transcriptional programs contribute to altered neural maturation, motor dysfunction and death. However, mice survive to young adulthood, in part from Snf2l compensation that restores Engrailed-1 expression. Similarly, Purkinje-specific Snf2h ablation affects chromatin ultrastructure and dendritic arborization, but alters cognitive skills rather than motor control. Our studies reveal that Snf2h controls chromatin organization and histone H1 dynamics for the establishment of gene expression programs underlying cerebellar morphogenesis and neural maturation.
- MeSH
- Adenosine Triphosphatases metabolism MeSH
- Analysis of Variance MeSH
- Bromodeoxyuridine MeSH
- Chromatin Immunoprecipitation MeSH
- Chromosomal Proteins, Non-Histone metabolism MeSH
- Fluorescence MeSH
- Galactosides MeSH
- Histones metabolism MeSH
- Homeodomain Proteins metabolism MeSH
- In Situ Hybridization MeSH
- Immunohistochemistry MeSH
- Indoles MeSH
- In Situ Nick-End Labeling MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Rotarod Performance Test MeSH
- Microarray Analysis MeSH
- Morphogenesis genetics physiology MeSH
- Cerebellum embryology MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Neural Stem Cells metabolism physiology MeSH
- Image Processing, Computer-Assisted MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Purkinje Cells metabolism MeSH
- Chromatin Assembly and Disassembly physiology MeSH
- Tolonium Chloride MeSH
- Microscopy, Electron, Transmission MeSH
- Gene Expression Regulation, Developmental genetics physiology MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- MeSH
- Staining and Labeling MeSH
- Adult MeSH
- Research Support as Topic MeSH
- Middle Aged MeSH
- Humans MeSH
- Mast Cells pathology MeSH
- Image Processing, Computer-Assisted MeSH
- Tolonium Chloride MeSH
- Varicose Veins pathology MeSH
- Saphenous Vein pathology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- MeSH
- Alcian Blue diagnostic use MeSH
- Autoradiography methods utilization MeSH
- Staining and Labeling methods utilization MeSH
- Glycosaminoglycans chemistry metabolism MeSH
- Histocytochemistry methods utilization MeSH
- Calcinosis diagnosis metabolism MeSH
- Myocytes, Cardiac metabolism pathology MeSH
- Rabbits MeSH
- Sulfates diagnostic use chemistry MeSH
- Heart Valves chemistry metabolism MeSH
- Statistics as Topic MeSH
- Tolonium Chloride diagnostic use MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
