This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptor antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100 mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20 mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.
- MeSH
- Biosensing Techniques MeSH
- Dextromethorphan analysis chemistry metabolism MeSH
- Electrophoresis, Capillary instrumentation methods MeSH
- Microsomes, Liver metabolism MeSH
- Humans MeSH
- Spectrophotometry, Ultraviolet MeSH
- Cytochrome P-450 Enzyme System metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il. ; 32 cm + 1 volná příloha
Projek se zabývá vztahy mezi imunologickými faktory a metabolismem léčiv. Na preklinické a molekulární úrovni budou studovány mechanismy změn vyvolaných imunostimulátory a imunosupresivy v oxidativním a acetilačním metabolismu.
- MeSH
- Acecainide analysis MeSH
- Dextromethorphan analysis MeSH
- Dextrorphan analysis MeSH
- Data Interpretation, Statistical MeSH
- Procainamide analysis MeSH
- Laboratory Animal Science MeSH
- Chromatography, High Pressure Liquid utilization methods MeSH
- Conspectus
- Lékařské vědy. Lékařství
- NML Fields
- farmacie a farmakologie
- alergologie a imunologie
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
