Nucleus pulposus cells (NPC) are important for the development of intervertebral disc degeneration (IVDD). miR-4478 can aggravate IVDD, but whether it can aggravate IVDD by regulating ferroptosis in NPC remains unclear. The optimal level of ferroptosis activator RSL3 for eliciting ferroptosis in NPC was screened by Western blot and CCK-8 assay. The targeting relationship between miR-4478 and its potential target solute carrier family 7 member 11 (SLC7A11) was explored based on dual luciferase assay. On this basis, IVDD models were constructed. After over-expression or knockdown of miR-4478 or SLC7A11, CCK-8 and calcein-AM/PI assays were employed to evaluate cell damage. Flow cytometry, Western blot and Prussian blue staining were employed to evaluate oxidation and ferroptosis levels, and histopathological staining was applied to evaluate the intervertebral disc tissue injury degree. The optimal concentration of RSL3 was 1 μM. Under these conditions, miR-4478 or SLC7A11 can be effectively over-expressed or knocked down after transfection. Knockdown of miR-4478 can improve the survival rate of NPC, the level of Fe2+ ions, improve the pathological damage of intervertebral disc structure, reduce iron deposition in tissues, and significantly reduce expression of reactive oxygen species (ROS) and ferroptosis-related protein. The levels of antioxidant enzymes were significantly increased. When miR-4478 was over-expressed, the above phenomenon was reversed. On this basis, after SLC7A11 was over-expressed, the effect of miR-4478 up-regulation was weakened, and NPC ferroptosis was improved. miR-4478 can target SLC7A11 to promote NPC damage, peroxide accumulation and iron metabolism disorders, leading to ferroptosis, thereby inducing IVDD.
- MeSH
- degenerace meziobratlové ploténky * metabolismus genetika patologie MeSH
- ferroptóza * genetika MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- mikro RNA * metabolismus genetika MeSH
- nucleus pulposus * metabolismus patologie cytologie MeSH
- potkani Sprague-Dawley MeSH
- transportní systém aminokyselin y+ * metabolismus genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Quantitative analysis of the trace element content of human intervertebral discs (IVDs) is essential because it can identify specific enzymes or metabolites that may be related to human intervertebral disc degeneration (IVDD). The goal of this study was to assess the concentrations of copper (Cu), iron (Fe), manganese (Mn), lead (Pb), zinc (Zn), sodium (Na), magnesium (Mg), potassium (K), phosphorus (P), and calcium (Ca) in serum samples obtained from patients with IVDD in comparison to healthy volunteers (a control group). The study group consisted of 113 Caucasian patients qualified by a specialist neurosurgeon for microdiscectomy. The control group consisted of 113 healthy volunteers who met the eligibility criteria for blood donors. The examined clinical material was the serum samples obtained from both groups.Based on the quantitative analysis of selected elements, there were statistically significantly (p 0.05) higher concentrations of Cu (1180 μg/L±800 μg/L vs. 1230 μg/L±750 μg/L), Zn (790 μg/L±300 μg/L vs. 850 μg/L±200 μg/L), and Mg (21730 μg/L±4360 μg/L vs. 23820 μg/L±4990 μg/L) in the serum of healthy volunteers compared to those in the study group. In addition, statistically significant changes were not detected in the concentrations of any elements among either sex in either the study or control group or in their body mass index (BMI) values (p > 0.05). In the serum samples from the study group, the strongest relationships were noted between the concentrations of Zn and Pb (r = 0.61), Zn and P (r = 0.69), Zn and Ca (r = 0.84), Zn and Cu (r = 0.83), Mg and Ca (r = 0.74), and Ca and P (r = 0.98).It has been indicated that, above all, the concentrations of Cu, Zn, Ca, and Mg depend on the advancement of radiological changes, according to the Pfirrmann scale. However, no influence on pain intensity was found, depending on the concentration of the assessed elements.The analysis indicates that the determination of serum Cu, Zn, Ca, and Mg concentrations may have diagnostic significance in predicting the onset of lumbosacral IVDD. The predictive evaluation of changes in the concentrations of selected elements in patients with degenerative lumbar IVD lesions appears to be a promising, cost-effective strategy.
BACKGROUND: Midline lumbar fusion (MIDLF) is one promising new surgical technique that has been developed to minimize perioperative damage to the paravertebral stabilizing musculotendinous system. The aim of this study was to assess long-term clinical and radiological effects of MIDLF. METHODS: This prospective cohort study evaluated patients who underwent MIDLF for degenerative spinal instability. Clinical and radiological examinations were performed before and after surgery. Perioperative and postoperative complications were recorded. Follow-up was 2 years. P ≤ 0.05 was considered statistically significant. RESULTS: The study included 64 patients (mean age 58.9 ± 10.7 years; 41 women [64.1%]). The most frequent indication for MIDLF was degenerative spondylolisthesis grade I (28 cases [43.8%]); the prevalent spinal segment to be fused was L4-L5 (35 cases [54.7%]). Mean duration of surgery was 148.2 ± 28.9 minutes. Relief of low back pain and leg pain was significant and stable in the postoperative period as assessed by visual analog scale (P < 0.001). Of patients, 86.9% reported fair, good, or excellent outcomes in terms of pain relief based on MacNab score 2 years after surgery. Patients' level of function in activities of daily living improved significantly based on Oswestry Disability Index score: from 66.8 ± 9.8 before surgery to 33.9 ± 16.5 2 years after surgery (P < 0.001). X-rays and computed tomography at 12 months showed interbody fusion in 46 cases (73.4%), inconclusive results in 13 cases (20.3%), and no fusion in 4 cases (6.3%). No damage to neural or vascular structures and no failure of hardware or screw loosening were recorded. CONCLUSIONS: MIDLF is a safe, efficient method for surgical treatment of lumbar spine instability. Its limited invasiveness contributes to better preservation of paravertebral muscles and enhanced postoperative spinal stability.
- MeSH
- bederní obratle diagnostické zobrazování patologie chirurgie MeSH
- degenerace meziobratlové ploténky diagnostické zobrazování patologie chirurgie MeSH
- dospělí MeSH
- fúze páteře * MeSH
- lidé středního věku MeSH
- lidé MeSH
- prospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Fyzická záťaž, ktorá má priamy vplyv na excesívne zvyšovanie intradiskálneho tlaku, môže byť jedným z hlavných rizikových faktorov pre vznik degeneratívnych zmien medzistavcovej platničky. Rekreačné aj vrcholové športové aktivity znamenajú opakované zvyšovanie vnútroplatničkového tlaku, čo môže iniciovať degeneratívne zmeny medzistavcovej platničky. Rozličné typy športov vytvárajú aj rozličné pôsobenie priamych síl na platničkové štruktúry. Na druhej strane sa šport a športový tréning v populácii všeobecne považuje za zdraviu prospešný. Mnohí lekári pokladajú športový tréning za formu prevencie proti poškodeniu chrbtice. Autori v nasledujúcom texte analyzujú viaceré športové aktivity s rozličným typom pôsobenia priamych síl na chrbticu. Cieľom je informovať o realistických vedeckých faktoch týchto športových aktivít na degeneráciu medzistavcovej platničky a zároveň aj o dostupných faktoch, ktoré potvrdzujú pozitívny efekt určitého typu športovej záťaže v rámci prevencie poškodenia medzistavcovej platničky.
Physical strain, which has a direct influence on the excessive increase of intra-discal pressure, is possibly one of the main risk factors in the for-mation of degenerative changes of the intervertebral discs. Recreational and professional sport activities cause repetitive increase of intra-discal pressure, which can initiate degenerative changes of the intervertebral disc. Different types of sport create various effects of direct forces on the disc structures. On the other hand, sport and exercise is in general regarded as beneficial for our health. Many physicians also regard sport and exercise as a form of prevention against back injuries. The authors analyse numerous sport activities with different types of direct forces acting on the spine, with the goal to inform about realistic scientific facts regarding the impact of these activities on the degeneration of the intervertebral disc. Moreover, they inform about the available facts, which confirm the positive effects of certain types of exercise load in the prevention of intervertebral disc damage.
- MeSH
- degenerace meziobratlové ploténky * epidemiologie patofyziologie patologie MeSH
- lidé MeSH
- lyžování MeSH
- meziobratlová ploténka * patofyziologie patologie MeSH
- nemoci páteře MeSH
- páteř patofyziologie patologie MeSH
- plavání MeSH
- sporty * MeSH
- vzpírání MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Cervikokraniální (CC) syndrom (cervikogenní bolest hlavy) je přenesená bolest hlavy z oblasti krční páteře. Jde typicky o asymetrické a někdy i jednostranné bolesti, které mohou být provokovány pohybem krku, nevhodnou polohou hlavy nebo tlakem na spoušťové body na krku. Jsou prezentována současná diagnostická kritéria Mezinárodní společnosti pro bolesti hlavy a Mezinárodní studijní skupiny pro cervikogenní bolesti hlavy. V diferenciální diagnóze je třeba vyloučit sekundární organické příčiny, zejména expanzivní procesy v zadní jámě lební nebo subarachnoidální krvácení. Neurologické vyšetření u CC syndromu by mělo být normální a speciální manuální myoskeletální vyšetření často prokáže abnormitu krční páteře. Zobrazovací vyšetření výpočetní tomografií (CT) nebo magnetickou rezonancí (MR) je důležité právě pro diagnostiku sekundárních příčin bolesti, kde jsou indikovány jiné terapeutické postupy. Úspěšná léčba CC syndromu obvykle vyžaduje komplexní multifaktoriální přístup s využitím farmakologické léčby i nefarmakologických postupů (rehabilitační a manuální terapie).
Cervicogenic headache is a referred pain from the cervical spine. It is typically an asymmetric or unilateral headache that can be provoked by neck movement, awkward head positions or pressure on tender points in the neck. The current International Headache Society and Cervicogenic Headache International Study Group diagnostic criteria are presented. In the differential diagnosis secondary organic disorders such as a space-occupying lesion particularly in the posterior cranial fossa or subarachnoid hemorrhage should be excluded. Neurologic examination in cervicogenic headache should be normal and special manual musculoskeletal assessment will most often suggest an underlying cervical spine abnormality. Computed tomography (CT) or magnetic resonance imaging (MRI) is primarily used to search for secondary causes of pain that may require other form management. The successful treatment of cervicogenic headache usually requires a complex multifactorial approach using pharmacological treatment, and non-pharmacological approaches (rehabilitation and manual therapy).
- Klíčová slova
- cervikogenní bolest hlavy, diagnostická kritéria, krční páteř, fasetové klouby, cervikální závrať, léčba,
- MeSH
- bolest etiologie MeSH
- degenerace meziobratlové ploténky komplikace patologie MeSH
- diferenciální diagnóza MeSH
- farmakoterapie MeSH
- krk inervace patofyziologie patologie MeSH
- lidé MeSH
- management bolesti MeSH
- nociceptory fyziologie MeSH
- páteř inervace patofyziologie patologie MeSH
- posttraumatická bolest hlavy diagnóza etiologie patofyziologie MeSH
- techniky fyzikální terapie MeSH
- závrať etiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
