BACKGROUND: Peritoneal larval cestodiasis induced by Mesocestoides Vaillant, 1863 (Cyclophyllidea: Mesocestoididae) is a common cause of severe infections in domestic dogs and cats, reported also from other mammals and less frequently from birds. However, there is a limited knowledge on the taxonomy of causative agents of this disease. RESULTS: In the present study, we investigated a massive, likely lethal, infection of a song thrush Turdus philomelos (Passeriformes: Turdidae) by Mesocestoides sp. tetrathyridia. We performed combined morphological and phylogenetic analysis of the tetrathyridia and compared them with the materials obtained previously from other birds and mammals. The metrical data fitted within the wide range reported by previous authors but confirmed the limited value of morphological data for species identification of tetrathyridia of Mesocestoides spp. The molecular analyses suggested that the isolates represented an unidentified Mesocestoides sp. that was previously repeatedly isolated and sequenced in larval and adult forms from domestic dogs and cats in Europe, the Middle East and North Africa. In contrast to the present study, which found encysted tetrathyridia, four of the five previous studies that identified the same species described infections by acephalic metacestodes only. CONCLUSIONS: The tetrathyridia of the examined Mesocestoides sp. are described in the present study for the first time. However, the possible match with the species that were previously reported to infect birds remains uncertain. The phylogenetic analyses also suggested the rejection of two cases that were previously identified as Mesocestoides corti as they were likely caused by the same species as in the presently reported infection case. The newly provided DNA sequences should allow the assignment to species in the future, when adults of the genus Mesocestoides are more thoroughly sequenced.

• MeSH
• cysticerkóza přenos   veterinární   MeSH
• domácí zvířata parazitologie   MeSH
• fatální výsledek MeSH
• fylogeneze MeSH
• kočky MeSH
• Mesocestoides genetika   patogenita   MeSH
• nemoci koček parazitologie   přenos   MeSH
• nemoci psů parazitologie   přenos   MeSH
• psi MeSH
• savci parazitologie   MeSH
• sekvence nukleotidů MeSH
• zpěvní ptáci parazitologie   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Cieľ: Infekcia larvami pásomnice M. vogae spôsobuje fibrogenézu pečene hostiteľa v chronickej fáze ochorenia, ktorá sa nedá potlačiť používanými antihelmintikmi. Cieľom práce bolo overiť, či podávanie praziquantelu so silymarínom ovplyvní tento proces, pričom na hodnotenie sme použili biochemické markeryfibrogenézy. Metódy: Myši (samce, ICR)sa infikovali larvami M. vogae orálne (p.o.) a od 15. do 24. dňa po infekcii (p.i.) sa im podávalo (p.o.) buďantihelmintikum praziquntel (PZQ) samotné alebo v kombinácii s hepatopretektívom silymarínom (SIL). Neliečené infikované myši slúžili ako kontrola. Na biochemické stanovenia sa použilo sérum a pečeň, ktoré boli odobraté myšiam počas troch týždňov po liečbe (p.t.). Výsledky: U myší infikovaných larvami M. vogae sa po liečbe kombináciou PZQ a SIL zistil signifikantný (p < 0,05) vzostup antioxidačnej aktivity v pečeňovom parenchýme a regenerácia hepatocytov, pokles peroxidácie bunkových lipidov a fibrogenézy, ako aj zvýšenie efektívnosti liečby porovnaním s liečbou samotným PZQ. Závery: Zistilo sa, že silymarín výrazne znižuje množstvo reaktívnych foriem kyslíka produkovaných zápalovými bunkami počas infekcie M. vogae v pečeni myší, čím potláča peroxidáciu lipidov, prispieva k regenerácii hepatocytov a pôsobí antifibroticky. Získané výsledky poukazujú na potrebu súčasného a dlhodobého podávania antihelmintika s hepatoprotektívnou a antioxidačne pôsobiacou látkou, akou je napr. silymarín, počas parazitárnej infekcie spôsobujúcej fibrózu pečene.

Design: Tapeworm infection with larvae of Mesocestoides vogae causes hepatic fibrogenesis of the host during the chronic phase of disease, which cannot be eliminated using anthelmintic drugs. We investigated the effect of the anthelmintic drug praziquantel and the hepatoprotective agent silymarin on the efficacy of treatment and biochemical markers of fibrosis and liver damage/regeneration. Methods: Mice (males, ICR) were infected with M. vogae larvae orally (p.o). During days 15-24 post infection (p.i.) they were treated with anthelmintic praziquantel (PZQ) only or together with the hepatoprotective agent silymarin (SIL). Infected-untreated mice served as the control. Biochemical markers were analysed in the serum and livers of the mice collected within three weeks post therapy (p.t.). Results: It was found that in mice infected with M. vogae larvae during the follow-up of therapy with PZQ and SIL, the antioxidative capacity of the liver parenchyma and regeneration of hepatocytes was elevated significantly (p < 0.05), cell lipids peroxidation and fibrogenesis was suppressed and efficacy of the treatment increased in comparison with PZQ treatment alone. Conclusion: It was found that silymarin co-administration markedly reduces the amount of reactive oxygen species produced by inflammatory cells during M. vogae infection in the liver of mice, resulting in suppressed lipid peroxidation. Moreover, it contributes to the regeneration of hepatocytes, acts antifibrotically, and all these effects of SIL could contribute to the significantly enhanced larvicidal effect of PZQ. The obtained results showthat simultaneous and long-term administration of the anthelmintic drug and a hepatoprotective/antioxidant agent such as silymarin are necessary for more effective treatment of parasitic infections causing hepatic fibrosis.

• MeSH
• financování organizované MeSH
• glutathion MeSH
• hodnocení léčiv MeSH
• játra anatomie a histologie   účinky léků   MeSH
• kolagen MeSH
• kombinovaná farmakoterapie MeSH
• kyselina hyaluronová MeSH
• lipidové peroxidy MeSH
• Mesocestoides patogenita   účinky léků   MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední ICR MeSH
• myši MeSH
• parazitární onemocnění jater farmakoterapie   imunologie   MeSH
• praziquantel farmakologie   terapeutické užití   MeSH
• silymarin farmakologie   terapeutické užití   MeSH
• statistika jako téma MeSH
• transaminasy MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH