Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp=3μm, core-shell particles Kinetex C18, dp=2.6μm (100μm i.d.) and monolithic column Chromolith CapRod (100μm i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360nLmin(-1). Separation of studied compounds was achieved in less than 7min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3μm, 100mm×4.6mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace.
- MeSH
- acetaldehyd chemie MeSH
- acetonitrily chemie MeSH
- analýza potravin metody MeSH
- anthokyaniny chemie MeSH
- glukosidy chemie MeSH
- hmotnostní spektrometrie MeSH
- katechin chemie MeSH
- poréznost MeSH
- Vitis chemie MeSH
- vysokoúčinná kapalinová chromatografie * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Many aldehydes are respiratory irritants which can cause inflammation or other adverse reactions in the respiratory system after being inhaled. Depending on the type and amount of irritant compound inhaled, victims can exhibit symptoms ranging from minor respiratory discomfort to acute airway and lung injury and even death. The lungs are susceptible to many airborne irritants. There are hundreds of substances that can pollute air and harm lungs. Aldehydes are just one type of airborne pollutants that can adversely affect lungs. Some of these agents may be directly toxic. They are also strong irritants for the skin, eyes, and nasal passages. The vapor is irritating to the eyes and respiratory tract. Aldehydes as acrolein or glutaraldehyde are also corrosive substances.
- MeSH
- acetaldehyd chemie škodlivé účinky toxicita MeSH
- akrolein chemie škodlivé účinky toxicita MeSH
- aldehydy * chemie škodlivé účinky toxicita MeSH
- benzaldehydy chemie škodlivé účinky toxicita MeSH
- dráždivé látky chemie škodlivé účinky toxicita MeSH
- formaldehyd chemie škodlivé účinky toxicita MeSH
- glutaraldehyd chemie škodlivé účinky toxicita MeSH
- králíci MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- malondialdehyd chemie škodlivé účinky toxicita MeSH
- myši MeSH
- poruchy dýchání * chemicky indukované MeSH
- znečištění ovzduší škodlivé účinky MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
