Nasal immunisation represents an innovative and perspective route of vaccine administration that provides many benefits compared to the more traditional approaches. Since most infections start on mucosal membranes, the mucosal immunisation provides a rational reason for its application. Mucosal delivery for vaccine administration (for example oral or nasal routes) could stimulate both systemic and mucosal immune responses. However, there are still some limitations that should be solved for a broader utilisation of this approach. There is still the necessity to use strongly immunogenic antigens or appropriate adjuvants for the induction of a strong immune response. The use of nanoparticles in the vaccine development could represent a promising approach for the mucosal vaccine research. Nanoparticles could thus serve as delivery vehicles providing to vaccines their unique properties, such as the antigen stabilisation and protection, serve as an adjuvant and elicit an antigen-specific immune response on the target sites.

• MeSH
• antigeny imunologie   klasifikace   MeSH
• aplikace slizniční MeSH
• chitosan * aplikace a dávkování   chemická syntéza   chemie   farmakologie   imunologie   MeSH
• lidé MeSH
• nanočástice klasifikace   terapeutické užití   MeSH
• nanočásticový lékový transportní systém * terapeutické užití   MeSH
• nos imunologie   MeSH
• nosní sliznice imunologie   MeSH
• vakcinace metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH
• přehledy MeSH

Scaffolds can be considered as one of the most promising treatments for bone tissue regeneration. Herein, blends of chitosan, poly(vinyl alcohol), and hydroxyapatite in different ratios were used to synthesize scaffolds via freeze-drying. Mechanical tests, FTIR, swelling and solubility degree, DSC, morphology, and cell viability were used as characterization techniques. Statistical significance of the experiments was determined using a two-way analysis of variance (ANOVA) with p < 0.05. Crosslinked and plasticized scaffolds absorbed five times more water than non-crosslinked and plasticized ones, which is an indicator of better hydrophilic features, as well as adequate resistance to water without detriment of the swelling potential. Indeed, the tested mechanical properties were notably higher for samples which were undergone to crosslinking and plasticized process. The presence of chitosan is determinant in pore formation and distribution which is an imperative for cell communication. Uniform pore size with diameters ranging from 142 to 519 µm were obtained, a range that has been described as optimal for bone tissue regeneration. Moreover, cytotoxicity was considered as negligible in the tested conditions, and viability indicates that the material might have potential as a bone regeneration system.

• MeSH
• biokompatibilní materiály chemie   terapeutické užití   MeSH
• chitosan chemická syntéza   chemie   terapeutické užití   MeSH
• hydroxyapatit chemická syntéza   chemie   terapeutické užití   MeSH
• kosti a kostní tkáň chemie   MeSH
• lidé MeSH
• osteoblasty účinky léků   MeSH
• polyvinylalkohol chemická syntéza   chemie   terapeutické užití   MeSH
• proliferace buněk účinky léků   MeSH
• regenerace kostí účinky léků   MeSH
• tkáňové inženýrství * MeSH
• tkáňové podpůrné struktury chemie   MeSH
• vývoj kostí účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

An extremely simple procedure has been developed for the immobilization of Saccharomyces cerevisiae cells on magnetic chitosan microparticles. The magnetic carrier was prepared using an inexpensive, simple, rapid, one-pot process, based on the microwave irradiation of chitosan and ferrous sulphate at high pH. Immobilized yeast cells have been used for sucrose hydrolysis, hydrogen peroxide decomposition and the adsorption of selected dyes.

• MeSH
• barvicí látky metabolismus   MeSH
• biodegradace MeSH
• chitosan chemická syntéza   chemie   MeSH
• imobilizované buňky chemie   metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• magnetické jevy MeSH
• magnetismus metody   MeSH
• mikrovlny MeSH
• peroxid vodíku metabolismus   MeSH
• Saccharomyces cerevisiae chemie   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH

The present work aims to the development of innovative new derivatives of chitosan that can be used for medical applications. This innovation is based on the synthesis and characterization of chitosan-g-aminoanthracene derivatives. Thus, N-(anthracen-9-yl)-4,6-dichloro-[1,3,5]-triazin-2-amine (AT) reacted with chitosan by the following steps: at first, cyanuric chloride reacted with 9-aminoanthracene to obtain N-(anthracen-9-yl)-4,6-dichloro-[1,3,5]-triazin-2-amine (AT), then the AT reacted with chitosan to obtain (CH-g-AT). The final product of CH-g-AT was separated, purified and re-crystallized by dioxane. The structure of the prepared chitosan derivatives was confirmed by FTIR-ATR, solid-NMR, TGA, X-RD, and DSC. The new chitosan derivatives showed fluorescence spectra in liquid and in solid state as well. CH-g-AT showed also high antibacterial activity against gram -ve species (Escherichia coli).

• MeSH
• antibakteriální látky chemická syntéza   chemie   farmakologie   MeSH
• chitosan chemická syntéza   chemie   farmakologie   MeSH
• Escherichia coli účinky léků   MeSH
• fluorescenční barviva chemická syntéza   chemie   farmakologie   MeSH
• stabilita léku MeSH
• techniky syntetické chemie MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH