The virulence factors EAST1 and AIDA are often detected in ETEC/VTEC strains isolated from pigs and their role in diarrhoeal infections is discussed. In order to elucidate the pathogenesis of AIDA, the colonisation patterns of F4 positive and AIDA positive strains were investigated. Two wild-type Escherichia coli strains AIDA/EAST1 and F4/EAST1 isolated from diarrhoeal piglets were used for animal experiment to evaluate the ability of the EAST1 toxin to be involved in induction of diarrhoea. Gnotobiotic piglets were supplemented with normal porcine serum and orally inoculated with the strains. Faecal bacterial shedding of the challenge strains was observed during the experiment. Light microscopy and scanning electron microscopy were used to detect the colonisation pattern of both challenge strains. Although bacterial isolation demonstrated shedding of the challenge strains until the end of the experiment, diarrhoea did not develop in any piglet. Based on histological examination, piglets were more heavily colonised in the case of infection with E. coli O149/F4/EAST1 strain. Scanning electron microscopy showed bacterial cells of F4/EAST1 E. coli adhering to enterocytes, in contrast to AIDA/EAST1 which were poorly present on the intestinal surface. The EAST1 toxin alone was not able to induce diarrhoea in animals. Therefore our results demonstrate that the function/role of EAST1 and AIDA in colibacillosis of pigs remains to be elucidated.

• MeSH
• bakteriální adheziny imunologie   MeSH
• bakteriální toxiny imunologie   MeSH
• enterotoxiny imunologie   MeSH
• Escherichia coli imunologie   MeSH
• feces mikrobiologie   MeSH
• gnotobiologické modely imunologie   MeSH
• imunohistochemie veterinární   MeSH
• infekce vyvolané Escherichia coli imunologie   mikrobiologie   veterinární   MeSH
• mikroskopie elektronová rastrovací veterinární   MeSH
• náhodné rozdělení MeSH
• nemoci prasat imunologie   mikrobiologie   MeSH
• nemoci střev imunologie   mikrobiologie   veterinární   MeSH
• neparametrická statistika MeSH
• prasata MeSH
• proteiny z Escherichia coli imunologie   MeSH
• průjem imunologie   mikrobiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Hypersensitivity reactions limit the use of the antileukemic enzyme asparaginase (ASE). We evaluated Ab levels against Escherichia coli ASE and ASE activity in 1221 serum samples from 329 patients with acute lymphoblastic leukemia who had received ASE treatment according to the ALL-BFM 2000 or the ALL-REZ BFM 2002 protocol for primary or relapsed disease. ASE activity during first-line treatment with native E coli ASE and second-line treatment with pegylated E coli ASE was inversely related to anti-E coli ASE Ab levels (P < .0001; Spearman rank order correlation). An effect on ASE activity during second-line treatment with pegylated E coli ASE was, however, only observed when anti-E coli ASE Ab levels were high (> 200 AU/mL). In the presence of moderate or intermediate Ab levels (6.25-200 AU/mL) the switch from native to pegylated E coli ASE resulted in a significant increase of ASE activity above the threshold of 100 U/L (P < .05). Erwinia chrysanthemi ASE activity was not correlated with anti-E coli ASE Ab levels. Erwinia ASE was found to be the best ASE alternative if Ab levels against E coli ASE exceed 200 AU/mL. This retrospective analysis is the first to describe the relationship between the level of anti-E coli ASE Abs and serum activity of pegylated E coli ASE used second-line after native E coli ASE.

• MeSH
• adjuvantní chemoterapie MeSH
• akutní lymfatická leukemie farmakoterapie   krev   MeSH
• antitumorózní látky aplikace a dávkování   imunologie   terapeutické užití   MeSH
• asparaginasa aplikace a dávkování   imunologie   terapeutické užití   MeSH
• biomarkery farmakologické krev   MeSH
• daunomycin imunologie   terapeutické užití   MeSH
• dítě MeSH
• dospělí MeSH
• Escherichia coli enzymologie   imunologie   MeSH
• kojenec MeSH
• léková alergie diagnóza   imunologie   krev   MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• monitorování léčiv metody   MeSH
• prednison imunologie   terapeutické užití   MeSH
• předškolní dítě MeSH
• proteiny z Escherichia coli imunologie   MeSH
• protilátky krev   MeSH
• protokoly antitumorózní kombinované chemoterapie imunologie   terapeutické užití   MeSH
• randomizované kontrolované studie jako téma MeSH
• rekombinantní proteiny aplikace a dávkování   imunologie   terapeutické užití   MeSH
• retrospektivní studie MeSH
• vinkristin imunologie   terapeutické užití   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• předškolní dítě MeSH
• Publikační typ
• souhrny MeSH

Enterotoxigenic Escherichia coli (ETEC) is one of the most important causes of post-weaning diarrhea in piglets. Whilst serotype O149:F4 is frequently associated with hemorrhagic gastroenteritis, other serotypes have been found to be associated with mild or moderate enteritis. As neutrophils are recruited to sites of inflammation, the aim of this study was to ascertain whether or not there is any difference in the in vitro interaction between neutrophils and two different ETEC serotypes: O149:F4 and O147:F18. The association of bacteria with neutrophils was evaluated by flow cytometry. The respiratory burst was measured by the fluorescent probe dichlorofluorescein diacetate using flow cytometry and by L012-amplified chemiluminescence. The titers of antibodies against ETEC present in cultivation sera were assessed by agglutination. The viability of E. coli was ascertained by cultivation. It was found that the strains of O149 serotype were more frequently associated with neutrophils and induced a more intensive respiratory burst compared to the strains of O147 serotype. These differences might be due to the presence of different types of fimbriae on the surface of the strains tested and by the presence of anti-fimbrial antibodies in the porcine plasma. However, the intensive interaction between E. coli and the neutrophils and respiratory burst induced by the O149 strain did not lead to more efficient killing of the bacteria. It is suggested that a stronger respiratory burst may be an important factor causing severe clinical signs of post-weaning diarrhea in piglets.

• MeSH
• bakteriální fimbrie imunologie   MeSH
• enterotoxigenní Escherichia coli imunologie   MeSH
• infekce vyvolané Escherichia coli krev   imunologie   mikrobiologie   veterinární   MeSH
• nemoci prasat krev   imunologie   mikrobiologie   MeSH
• neutrofily imunologie   mikrobiologie   MeSH
• prasata MeSH
• proteiny z Escherichia coli krev   imunologie   MeSH
• průjem krev   imunologie   mikrobiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Since its discovery, DNA vaccination has become an effective strategy for the development of vaccines against cancer including cervical carcinoma (CC). The formation of CC is associated with human papillomavirus (HPV) infection. Viral E6 and E7 oncoproteins are suitable targets for therapeutic vaccination. To adapt the HPV16 E6 oncogene for DNA immunisation, we performed several modifications. First we fused the E6 gene with the 5' or 3'-terminus of the Escherichia coli beta-glucuronidase (GUS) gene and showed enhanced immunogenicity of the 3' fusion (GUS.E6). Then, as the E6 oncogene contains two alternative introns that result in the production of truncated forms of the E6 protein, we abolished the 5' splice site in the E6 gene. This modification completely eliminated the expression of the truncated E6 transcripts and thus increased the production of the full-length E6 protein. At the same time, it moderately reduced the immunogenicity of the modified non-fused (E6cc) or fused (GUS.E6cc) genes, probably as a consequence of the substitution in the immunodominant E6 epitope following the abolishment of the splice site. Furthermore, we reduced the oncogenicity of the E6 protein by two point mutations (E6GT) that, together, prevented E6-mediated p53 degradation. Finally, we constructed the GUS.E6GT gene characterized by enhanced safety and immunogenicity when compared with the wild-type E6 gene.

• MeSH
• bodová mutace MeSH
• buněčné linie MeSH
• cytokiny biosyntéza   MeSH
• DNA vakcíny genetika   imunologie   MeSH
• exprese genu MeSH
• glukuronidasa genetika   imunologie   MeSH
• introny MeSH
• leukocyty mononukleární imunologie   MeSH
• lidé MeSH
• lidský papilomavirus 16 genetika   imunologie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádory prevence a kontrola   MeSH
• onkogenní proteiny virové genetika   imunologie   MeSH
• proteiny z Escherichia coli genetika   imunologie   MeSH
• protilátky virové krev   MeSH
• rekombinantní fúzní proteiny genetika   imunologie   MeSH
• represorové proteiny genetika   imunologie   MeSH
• vakcíny proti papilomavirům genetika   imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH