BACKGROUND: Recent discoveries in cancer therapeutics have proven combination therapies more effective than individual drugs. This study describes the efficacy of the combination of Cinnamomum zeylanicum and doxorubicin against benzene-induced leukemia. METHODS AND RESULTS: Brine shrimp assay was used to assess the cytotoxicity of C. zeylanicum, doxorubicin and their combination. After AML induction in Sprague Dawley rats, the same drugs were given to rat groups. Changes in organ weight, haematological profile, and hepatic enzymes were determined. Real-time PCR was used to elucidate the effect on the expression of STMN1, GAPDH, P53 and various TRAIL and NF-kappaB components. C. zeylanicum reduced the cytotoxicity of doxorubicin. The combination treatment showed better anti-leukemic results than any of the individual drugs as evident from STMN1 expression (p < 0.001). It was particularly effective in reducing total white blood cell counts and recovering lymphocytes, monocytes and eosinophils along with hepatic enzymes ALT and AST (p < 0.001). All doses recovered relative organ weights and improved blood parameters. The combination therapy was particularly effective in inducing apoptosis, inhibition of proliferation marker GAPDH (p < 0.001) and NF-kappaB pathway components Rel-A (p < 0.001) and Rel-B (p < 0.01). Expressions of TRAIL components c-FLIP (p < 0.001), TRAIL ligand (p < 0.001) and caspase 8 (p < 0.01) were also altered. CONCLUSION: Cinnamomum zeylanicum in combination with doxorubicin helps to counter benzene-induced cellular and hepatic toxicity and improves haematological profile. The anti-leukemic effects are potentially due to inhibition of GAPDH and NF-kappa B pathway, and through regulation of TRAIL pathway. Our data suggests the use of C. zeylanicum with doxorubicin to improve anti-leukemic therapeutic regimes.

• MeSH
• apoptóza MeSH
• benzen farmakologie   MeSH
• doxorubicin farmakologie   MeSH
• krysa rodu rattus MeSH
• leukemie * farmakoterapie   MeSH
• NF-kappa B metabolismus   MeSH
• oleje prchavé * farmakologie   MeSH
• potkani Sprague-Dawley MeSH
• protein TRAIL metabolismus   farmakologie   MeSH
• skořicovník ceylonský metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• krevní glukóza * fyziologie   metabolismus   MeSH
• lidé MeSH
• metabolický syndrom * MeSH
• potraviny a nápoje * MeSH
• potraviny využití   MeSH
• preference v jídle MeSH
• prodejní automaty na potraviny MeSH
• sacharidy * aplikace a dávkování   farmakologie   škodlivé účinky   toxicita   MeSH
• skořicovník ceylonský * metabolismus   MeSH
• Check Tag
• lidé MeSH

• MeSH
• česnek metabolismus   MeSH
• chrom metabolismus   terapeutické užití   MeSH
• diabetes mellitus 2. typu * dietoterapie   MeSH
• hypoglykemika MeSH
• lidé MeSH
• metabolický syndrom terapie   MeSH
• psyllium terapeutické užití   MeSH
• rostlinné extrakty MeSH
• skořicovník ceylonský metabolismus   MeSH
• ženšen metabolismus   MeSH
• Check Tag
• lidé MeSH

Plant essential oils (EOs) have been reported to have health benefit properties and their preventive and therapeutic use in animals is expected to increase in the future. We evaluated the influence of five essential oils obtained from plant species which are known to have positive antimicrobial, antioxidative and anti-inflammatory effects – sage EO from Salvia officinalis L. (Lamiaceae), oregano EO from Origanum vulgare L. (Lamiaceae), thyme EO from Thymus vulgaris L. (Lamiaceae), clove EO from Syzygium aromaticum L. (Myrtaceae) and cinnamon EO from Cinnamomum zeylanicum Blume (Lauraceae) on the growth and development of mouse preimplantation embryos in vivo. Essential oils were added to commercial diet at concentrations of 0.25 % for sage EO, thyme EO, clove EO, cinnamon EO and 0.1 % for oregano EO, and fed to ICR female mice for 2 weeks ad libitum. Females were then mated with males of the same strain. Embryos obtained on Day 4 of pregnancy at the blastocyst stage were stained by morphological triple staining (Hoechst, PI, Calcein-AM) and evaluated using fluorescent microscopy. The effects of essential oils were estimated by the viability of embryos, number of nuclei and distribution of embryos according to nucleus number. Cinnamon EO significantly decreased the number of nuclei and the distribution of embryos according to nucleus number was significantly altered. Sage EO negatively influenced the distribution of embryos according to nucleus number. Clove and oregano EOs induced a significantly increased rate of cell death. Only thyme EO had no detectable effects on embryo development. In conclusion, none of the essential oils had any positive effect on embryo development, but some of them reduced the number of cells and increased the incidence of cell death.

• MeSH
• apoptóza genetika   MeSH
• dobromysl (rod) metabolismus   MeSH
• financování vládou MeSH
• implantace embrya fyziologie   MeSH
• myši inbrední ICR růst a vývoj   MeSH
• oleje rostlin metabolismus   terapeutické užití   MeSH
• šalvěj lékařská metabolismus   MeSH
• skořicovník ceylonský metabolismus   MeSH
• Syzygium metabolismus   MeSH
• Thymus (rostlina) metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

V posledních 5 letech byla publikována řada výsledků preklinických a klinických studií zabývajících se významem obsahových látek skořice jako antidiabetika. Byla vyslovena hypotéza o mechanizmu působení polymeru methylhydroxychalkonu jako inzulínotropního agens. Je významná podpora pro suplementaci diety diabetiků 2. typu skořicí. Skepse nad výsledky klinické studie se projevila pouze v jednom případě.

• MeSH
• alkaloidy farmakokinetika   izolace a purifikace   terapeutické užití   MeSH
• diabetes mellitus 2. typu farmakoterapie   MeSH
• hypoglykemika farmakokinetika   izolace a purifikace   terapeutické užití   MeSH
• lidé MeSH
• skořicovník ceylonský fyziologie   metabolismus   účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• klinické zkoušky MeSH

• MeSH
• diabetes mellitus 2. typu farmakoterapie   prevence a kontrola   terapie   MeSH
• glukagon farmakokinetika   metabolismus   účinky léků   MeSH
• hypoglykemika farmakokinetika   metabolismus   terapeutické užití   MeSH
• index tělesné hmotnosti MeSH
• lidé MeSH
• metabolický syndrom farmakoterapie   prevence a kontrola   terapie   MeSH
• skořicovník ceylonský metabolismus   MeSH
• Check Tag
• lidé MeSH