• MeSH
• Humans MeSH
• Pathology methods   MeSH
• Silver Proteins diagnostic use   MeSH
• Check Tag
• Humans MeSH

• Keywords
• protargol,
• MeSH
• Microscopy, Electron MeSH
• Epoxy Resins MeSH
• Glycogen MeSH
• Microscopy MeSH
• Silver Proteins MeSH

In this article we provide morphologic and morphometric data based on in vivo observation, protargol impregnation, scanning electron microscopy and an 18S rRNA gene sequence for another member of the genus Brachonella, Brachonella pulchra comb. nov. (basionym: Metopus pulcher Kahl, 1927). We also provide preliminary data on resting cyst structure and formation in Brachonella pulchra and discuss the possible taxonomic usefulness of these structures.

• MeSH
• Ciliophora classification   MeSH
• Phylogeny * MeSH
• Microscopy, Electron, Scanning MeSH
• RNA, Ribosomal, 18S genetics   MeSH
• Sequence Analysis, DNA MeSH
• Publication type
• Journal Article MeSH

• MeSH
• Pharmacy MeSH
• Pharmaceutical Preparations MeSH
• Publication type
• Review MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie

We studied morphological and molecular polymorphism of 53 Tetratrichomonas isolates obtained from amphibian, reptilian, mammalian hosts, and from a slug with the aid of protargol staining and analyses of ITS1-5.8S rRNA-ITS2, SSU rRNA, and alpha-tubulin gene sequences. The phylogenetic tree based on the concatenate of all sequences showed the monophyly of the genus Tetratrichomonas with respect to the genus Trichomonas. Our data suggest that two parabasalid genera, Pentatrichomonoides and Trichomonoides, may belong to the genus Tetratrichomonas. Tetratrichomonas isolates were divided into 16 robust host-specific and monophyletic groups that probably represent separate, mostly new, species. As only five Tetratrichomonas species were described from the examined host taxa so far, our study uncovered considerable species diversity within the genus. The wide host range, high level of species-specific host specificity, and newly revealed biodiversity make the genus Tetratrichomonas a valuable model for studying evolution of parasites.

• MeSH
• Bayes Theorem MeSH
• Financing, Organized MeSH
• Phylogeny MeSH
• Genetic Variation immunology   MeSH
• Host-Parasite Interactions genetics   MeSH
• DNA, Ribosomal Spacer genetics   MeSH
• Evolution, Molecular MeSH
• Molecular Sequence Data MeSH
• Mice parasitology   MeSH
• Swine parasitology   MeSH
• DNA, Protozoan genetics   chemistry   MeSH
• RNA, Ribosomal, 5.8S genetics   MeSH
• Sequence Analysis, DNA MeSH
• Trichomonadida genetics   classification   MeSH
• Animals MeSH
• Check Tag
• Mice parasitology   MeSH
• Animals MeSH

• MeSH
• Medicine MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Lékařské vědy. Lékařství
• NML Fields
• lékařství

Class Armophorea Lynn, 2004 includes two orders of mainly free-living anaerobic ciliates, Armophorida Jankowski, 1980 and Metopida Jankowski, 1980 and, a third, the exclusively endosymbiotic Clevelandellida. Kahl described the majority of free-living metopid species early in the 20th century. Excepting Jankowski in the 1960's, little further interest was shown in this group. Metopus turbo Dragesco and Dragesco-Kernéis, 1986, from a West African pond, was more recently described. Although not explicitly described as endemic, Dragesco believed in some degree of endemism of sub-Saharan ciliates. Our discovery of M. turbo on Guam, Micronesia was unexpected. Metopus turbo was identified by live observation, protargol impregnation, and scanning electron microscopy, providing reasonable evidence of conspecificity when compared with the original description. Outstanding morphologic features include the stout shape and transversely situated bandform macronucleus. The morphology of Metopus turbo differs markedly from that of the Metopus/Brachonella clade and is distant from Metopus es in phylogenetic analyses. The 18S rDNA sequence of the Guam M. turbo differs from that of M. es, type species of Metopus, by >8% and shows distinct morphologic differences from the genus Atopospira to which it is sister. These facts suggest that transfer to a new genus, Idiometopus gen. nov. is indicated.

• MeSH
• Ciliophora classification   isolation & purification   ultrastructure   MeSH
• Phylogeny MeSH
• Molecular Typing MeSH
• Fresh Water MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Africa MeSH
• Guam MeSH

• MeSH
• Inorganic Chemicals MeSH
• Anti-Infective Agents MeSH
• Antipyretics MeSH
• Drug Evaluation MeSH
• Pharmaceutical Preparations MeSH
• Organic Chemicals MeSH
• Gases MeSH
• Dietary Supplements MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie

• MeSH
• Drug Therapy MeSH
• Pharmaceutical Preparations MeSH

• Conspectus
• Farmacie. Farmakologie
• Učební osnovy. Vyučovací předměty. Učebnice
• NML Fields
• farmakoterapie
• farmacie a farmakologie
• NML Publication type
• učebnice vysokých škol

• MeSH
• Pharmacology MeSH
• Medicine MeSH
• Toxicology MeSH

• Conspectus
• Farmacie. Farmakologie
• Učební osnovy. Vyučovací předměty. Učebnice
• NML Fields
• farmacie a farmakologie
• NML Publication type
• učebnice vysokých škol