In the present study, the Salmonella typhimurium tester strain TA 100 was used in the plate-incorporation test to examine the antimutagenic potential of caffeic, ferulic and cichoric acids extracted from plant species of genera Echinacea (L) Moench, as well as of another phenolic acids, on 3-(5-nitro-2-furyl)acrylic acid (5NFAA) and sodium azide mutagenicity. All tested compounds possess antimutagenic activity. In the case of 5NFAA, the antimutagenic potency of tested compounds was in the order of gallic acid > ferulic acid > caffeic acid > syringic acid > vanillic acid. The mutagenic effect of sodium azide was inhibited by tested phenolic acids by about 20-35 %. The most effective compound, gallic acid inhibits this effect by 82 % in the concentration of 500 mug/plate. The only exception from favourable properties of tested phenolic acids is cichoric acid, which in the contrary significantly increased the mutagenic effect of 5NFAA.

• MeSH
• antimutagenní látky farmakologie   MeSH
• fenoly farmakologie   MeSH
• mutace účinky léků   MeSH
• mutageny farmakologie   MeSH
• rostlinné extrakty farmakologie   MeSH
• Salmonella typhimurium účinky léků   genetika   MeSH
• testy genotoxicity MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The effect of two phenolic compounds vanillin (4-hydroxy-3-methoxybenzaldehyde) and lignin on the development of drug/antibiotic resistance in Salmonella typhimurium was studied. Using the modified Ames test we have shown that vanillin alone has negligible effect on spontaneous mutability to ciprofloxacin and gentamicin resistance. At the tested concentrations vanillin reduces the toxicity of 4-nitroquinoline-N-oxide (4NQO) and reduces the ability of this compound to induce mutations leading to ciprofloxacin but not to gentamicin resistance. Lignin at higher concentrations increases mutagenicity to ciprofloxacin resistance and possess considerable inhibition effect on the spontaneous and 4NQO induced mutability to gentamicin resistance.

• MeSH
• bakteriální léková rezistence účinky léků   genetika   MeSH
• benzaldehydy farmakologie   MeSH
• ciprofloxacin farmakologie   MeSH
• fenoly farmakologie   MeSH
• gentamiciny farmakologie   MeSH
• lignin farmakologie   MeSH
• mutace účinky léků   MeSH
• Salmonella typhimurium účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

AIM: The purpose of this study was to determine how various compounds known to be positive mutagens, contribute to the development of mutations leading to ciprofloxacin resistance in Salmonella enterica subsp. enterica serotype Typhimurium. The molecular mechanism of ciprofloxacin resistance in treated strains was investigated. METHODS: A modified version of the incorporation plate test was used for quantitative determination of ciprofloxacin resistant mutants and for assessment of the mutation frequency induced by the positive mutagens in different concentrations. An AS-PCR-RFLP for monitoring of gyrA mutations was applied. RESULTS: Mutation frequency, expressed as number of antibiotic resistant colonies per 10(8) viable cells, was much higher after exposure of bacterial cells to 3-(5-nitro-2-furyl) acrylic acid and 2-nitrofluorene. All isolated cultures retain decreased susceptibility to antibiotic after multiple passages in antibiotic-free medium. 2-nitrofluorene was the best inducer of mutations in gyrA and in regulation genes affecting suppression of synthesis of outer membrane porins. 3-(5-nitro-2-furyl) acrylic acid gives rise to overproduction of efflux pump. CONCLUSIONS: The data suggest that antibiotic resistance may not be only a consequence of misuse of antibiotics. A polluted environment as well as food processing could contribute to this unwanted process.

• MeSH
• akryláty farmakologie   MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence genetika   MeSH
• bakteriální proteiny genetika   MeSH
• ciprofloxacin farmakologie   MeSH
• DNA gyráza genetika   MeSH
• fluoreny farmakologie   MeSH
• látky znečišťující vzduch farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• mutace účinky léků   MeSH
• mutační rychlost MeSH
• mutageny farmakologie   MeSH
• nitrofurany farmakologie   MeSH
• poriny biosyntéza   MeSH
• proteiny vnější bakteriální membrány biosyntéza   MeSH
• regulace genové exprese u bakterií MeSH
• Salmonella typhimurium účinky léků   genetika   MeSH
• Publikační typ
• časopisecké články MeSH

Biocides are very effective against huge spectrum of microorganisms and are used in wide range. Recent research pointed out on fact that there could be connection between use of biocides and development of antibiotic resistance. This paper assesses long-term effect of common biocides in subinhibitory concentrations on the development of antibiotic resistance in Enterococcus faecalis. We have studied effect of three common biocides – NaClO, ClO2 and H2O2/Ag. Obtained data showed, that all biocides decreased susceptibility of E. faecalis to ampicillin. In case of H2O2/Ag also gentamicin resistance has developed after long-term treatment. On the other hand, this biocide has caused loss of vancomycin resistance in E. faecalis. This suggests very important fact; however vancomycin resistant enterococci are according to WHO classified as pathogens with high priroty.

• Klíčová slova
• subinhibiční koncentrace, křížová rezistence,
• MeSH
• ampicilin MeSH
• antibakteriální látky klasifikace   MeSH
• bakteriální léková rezistence * účinky léků   MeSH
• ciprofloxacin MeSH
• dezinficiencia * klasifikace   MeSH
• Enterococcus faecalis účinky léků   MeSH
• gentamiciny MeSH
• mikrobiální testy citlivosti MeSH
• vankomycin MeSH
• výzkum MeSH

• MeSH
• bakteriální léková rezistence * MeSH
• kongresy jako téma MeSH
• Publikační typ
• zprávy MeSH

• MeSH
• amarant * MeSH
• ampicilin * MeSH
• antibakteriální látky MeSH
• antibiotická rezistence * MeSH
• ciprofloxacin * analogy a deriváty   MeSH
• fenoly MeSH
• jedlá semena MeSH
• lidé MeSH
• mutace * MeSH
• mutageny MeSH
• rostlinné extrakty * MeSH
• Salmonella typhimurium * MeSH
• Staphylococcus aureus * MeSH
• Check Tag
• lidé MeSH

• MeSH
• kongresy jako téma MeSH
• přenos infekční nemoci * MeSH
• znečištění vody * MeSH
• Publikační typ
• zprávy MeSH

The effect of H(2)O(2) on the induction of ciprofloxacin (CFL) resistant mutants of Salmonella enterica subsp. enterica serovar Typhimurium was evaluated and determinants of CFL resistance in the mutants were analyzed. Factors associated with CFL resistance in H(2)O(2)-induced mutants included (i) mutations in gyrA gene, predominantly (63 %) Asp(87)-->Asn and less (37 %) Ser(83)-->Phe substitutions, (ii) mutations in the regulatory genes of MarRAB or SoxRS or in the individual structural genes of these operons. Such mutations are induced by H(2)O(2) in a much lower extent. Reduced OmpF expression simultaneously with enhanced efflux was detected only in one mutant strain and 20 % of mutant strains had increased CFL efflux from the cells.

• MeSH
• antibakteriální látky farmakologie   metabolismus   MeSH
• bakteriální léková rezistence MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• ciprofloxacin farmakologie   metabolismus   MeSH
• DNA gyráza genetika   metabolismus   MeSH
• missense mutace MeSH
• mutageneze MeSH
• peroxid vodíku farmakologie   MeSH
• Salmonella typhimurium genetika   metabolismus   účinky léků   MeSH

• Publikační typ
• abstrakt z konference MeSH