Five poly(betaine) brushes were prepared, and their resistance to blood plasma fouling was studied. Two carboxybetaines monomers were copolymerized with 2-hydroxyethyl methacrylate (HEMA) to prepare novel hydrogels. By increasing the content of the zwitterionic comonomer, a 4-fold increase in the water content could be achieved while retaining mechanical properties close to the widely used poly(HEMA) hydrogels. All hydrogels showed an unprecedentedly low fouling from blood plasma. Remarkably, by copolymerization with 10 mol % of carboxybetaine acrylamide, hydrogels fully resistant to blood plasma were prepared.

• MeSH
• akrylamidy chemická syntéza   MeSH
• betain chemie   MeSH
• biokompatibilní materiály chemie   MeSH
• hydrogely chemická syntéza   MeSH
• krevní plazma chemie   MeSH
• lidé MeSH
• methakryláty chemická syntéza   MeSH
• polymerizace MeSH
• polymery chemie   MeSH
• povrchová plasmonová rezonance MeSH
• povrchové vlastnosti MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• voda chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In the current study, well-defined polymer brushes are shown as an effective surface modification to resist the adhesion of whole blood and its components. Poly[oligo(ethylene glycol)methylether methacrylate] (poly(MeOEGMA)), poly(hydroxyethyl methacrylate) (poly(HEMA)), poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)), and poly(carboxybetaine acrylamide) (poly(CBAA)) brushes were grown by surface initiated atom transfer radical polymerization (SI-ATRP) and subsequently characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), dynamic contact angle measurements, atomic force microscopy (AFM), and surface plasmon resonance (SPR) spectroscopy. All brushes decreased the fouling from blood plasma over 95% and prevented the adhesion of platelets, erythrocytes, and leukocytes as evidenced by SPR and SEM measurements.

• MeSH
• biokompatibilní potahované materiály chemie   MeSH
• bioznečištění MeSH
• buněčná adheze MeSH
• fotoelektronová spektroskopie MeSH
• krev metabolismus   MeSH
• lidé MeSH
• mikroskopie atomárních sil MeSH
• plazma bohatá na destičky MeSH
• polymerizace MeSH
• polymery chemická syntéza   chemie   MeSH
• povrchové vlastnosti MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• testování materiálů přístrojové vybavení   MeSH
• voda MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Poly(ϵ-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell-matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion.

• MeSH
• bioznečištění prevence a kontrola   MeSH
• buněčná adheze MeSH
• kultivované buňky MeSH
• myši MeSH
• nanovlákna chemie   MeSH
• polyestery * MeSH
• testování materiálů MeSH
• tkáňové inženýrství MeSH
• vazba proteinů MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.

• MeSH
• hydrogely chemie   MeSH
• methakryláty chemie   MeSH
• poréznost MeSH
• skot MeSH
• tkáňové inženýrství * MeSH
• tkáňové podpůrné struktury chemie   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Currently, one of the most promising treatments of lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed on the artificial surface of membranes which leads to activation of coagulation impairing effective scavenging of the endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) to specifically scavenge LPS the most common endotoxin. Poly[( N-(2-hydroxypropyl) methacrylamide)- co-(carboxybetaine methacrylamide)] brushes were grafted from poly(glycidyl methacrylate) microparticles using photoinduced single-electron transfer living radical polymerization (SET-LRP). Notably, only parts-per-million of copper catalyst were necessary to achieve brushes able to repel adsorption of proteins from blood plasma. The open porosity of the particles, accessible to polymerization, enabled us to immobilize sufficient PMB to selectively scavenge LPS from blood plasma.

• MeSH
• adsorpce MeSH
• akrylamidy metabolismus   MeSH
• biokompatibilní potahované materiály farmakologie   MeSH
• bioznečištění prevence a kontrola   MeSH
• epoxidové sloučeniny metabolismus   MeSH
• krevní plazma metabolismus   MeSH
• lidé MeSH
• lipopolysacharidy metabolismus   MeSH
• methakryláty metabolismus   MeSH
• polymerizace účinky léků   MeSH
• polymery chemie   MeSH
• polymyxin B farmakologie   MeSH
• povrchové vlastnosti účinky léků   MeSH
• proteiny metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Implantable sensor devices require coatings that efficiently interface with the tissue environment to mediate biochemical analysis. In this regard, bioinspired polymer hydrogels offer an attractive and abundant source of coating materials. However, upon implantation these materials generally elicit inflammation and the foreign body reaction as a consequence of protein fouling on their surface and concomitant poor hemocompatibility. In this report we investigate a strategy to endow chitosan hydrogel coatings with antifouling properties by the grafting of polymer brushes in a "grafting-from" approach. Chitosan coatings were functionalized with polymer brushes of oligo(ethylene glycol) methyl ether methacrylate and 2-hydroxyethyl methacrylate using photoinduced single electron transfer living radical polymerization and the surfaces were thoroughly characterized by XPS, AFM, water contact angle goniometry, and in situ ellipsometry. The antifouling properties of these new bioinspired hydrogel-brush coatings were investigated by surface plasmon resonance. The influence of the modifications to the chitosan on hemocompatibility was assessed by contacting the surfaces with platelets and leukocytes. The coatings were hydrophilic and reached a thickness of up to 180 nm within 30 min of polymerization. The functionalization of the surface with polymer brushes significantly reduced the protein fouling and eliminated platelet activation and leukocyte adhesion. This methodology offers a facile route to functionalizing implantable sensor systems with antifouling coatings that improve hemocompatibility and pave the way for enhanced device integration in tissue.

• MeSH
• aktivace trombocytů účinky léků   MeSH
• biokompatibilní potahované materiály chemie   farmakologie   MeSH
• biosenzitivní techniky metody   MeSH
• buněčná adheze účinky léků   MeSH
• chitosan chemie   MeSH
• hydrogely chemie   farmakologie   MeSH
• implantabilní infuzní pumpy MeSH
• leukocyty cytologie   účinky léků   MeSH
• lidé MeSH
• methakryláty chemie   MeSH
• polyethylenglykoly chemie   MeSH
• polymerizace MeSH
• primární buněčná kultura MeSH
• trombocyty účinky léků   MeSH
• volné radikály MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH