Linseed
Dotaz
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- MeSH
- geneticky modifikované rostliny MeSH
- len genetika MeSH
- lněný olej MeSH
- Geografické názvy
- Česká republika MeSH
Linseed represents a rich source of nutritional, functional and health-beneficial compounds. Nevertheless, the chemical composition and content of bioactive compounds may be quite variable and potentially affected by various factors, including genotype and the environment. In this study, the proximate chemical composition, lignans content and antioxidant potential of six experimentally grown linseed cultivars were assessed and compared. A diagonal cultivation trial in the University of South Bohemia Experimental Station in České Budějovice, Czech Republic, was established in three subsequent growing seasons (2018, 2019 and 2020). The results showed that the cultivar and growing conditions influenced most studied parameters. The lack of precipitation in May and June 2019 negatively affected the seed yield and the level of secoisolariciresinol diglucoside but did not decrease the crude protein content, which was negatively related to the oil content. The newly developed method for lignans analysis allowed the identification and quantification of secoisolariciresinol diglucoside and matairesinol. Their content correlated positively with the total polyphenol content and antioxidant assays (DPPH and ABTS radical scavenging activity), indicating the significant contribution to the biofunctional properties of linseed. On the other hand, we did not detect minor linseed lignans, pinoresinol and lariciresinol. The results of this study showed the importance of cultivar and growing conditions factors on the linseed chemical composition and the lignans content, determining its nutritional and medicinal properties.
- Klíčová slova
- oleje zastudena lisované, makový olej, konopný olej, avokádový olej, dýňový olej,
- MeSH
- fyziologie výživy MeSH
- lněný olej MeSH
- oleje * MeSH
- tuky škodlivé účinky MeSH
- zdraví MeSH
- Klíčová slova
- Johanna Budwig,
- MeSH
- fyziologie výživy MeSH
- komplementární terapie metody MeSH
- lněný olej terapeutické užití MeSH
- Publikační typ
- populární práce MeSH
A new method, reversed phase liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry (RPLC-SALDI MS) for the determination of brassicasterol (BR), cholesterol (CH), stigmasterol (ST), campesterol (CA) and β-sitosterol (SI) in oil samples has been developed. The sample preparation consisted of alkaline saponification followed by extraction of the unsaponificable fraction with diethyl ether. The recovery of the sterols ranged from 91 to 95% with RSD less than 4%. Separation of the five major sterols on a C18 column using methanol-water gradient was achieved in about 10min. An on-line UV detector was employed for the initial sterol detection prior to effluent deposition using a laboratory-built spotter with 1:73 splitter. Off-line SALDI MS was then applied for mass determination/identification and quantification of the separated sterols. Ionization of the nonpolar analytes was achieved by silver ion cationization with silver nanoparticles used as the SALDI matrix providing limits of detection 12, 6 and 11fmol for CH, ST and SI, respectively. Because of the incorporated splitter, the effective limits of detection of the RPLC-SALDI MS analysis were 4, 3 and 4pmol (or 0.08, 0.06 and 0.08μg/mL) for CH, ST and SI, respectively. For quantification, 6-ketocholestanol (KE) was used as the internal standard. The method has been applied for the identification and quantification of sterols in olive, linseed and sunflower oil samples. The described off-line coupling of RPLC to SALDI MS represents an alternative to GC-MS for analysis of nonpolar compounds.
- MeSH
- cholestadienoly chemie izolace a purifikace MeSH
- cholesterol analogy a deriváty chemie izolace a purifikace MeSH
- chromatografie s reverzní fází metody normy MeSH
- fytosteroly chemie izolace a purifikace MeSH
- ketocholesteroly chemie izolace a purifikace MeSH
- lněný olej analýza chemie MeSH
- oleje rostlin analýza chemie MeSH
- referenční standardy MeSH
- sitosteroly chemie izolace a purifikace MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody normy MeSH
- stigmasterol chemie izolace a purifikace MeSH
- stříbro chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
