Metallomics
Dotaz
Zobrazit nápovědu
sv.
sv.
- MeSH
- stopové prvky * MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
The researchers from Laboratory of Metallomics and Nanotechnologies at Department of Chemistry and Biochemistry of Mendel University in Brno were, in connection to their research priorities, interested in the idea to create a cooperating partner network joining the excellent central European laboratories focused on the metallomic research. The effort in this area was supported by the project Metallomic Scientific Network of The International Visegrad Fund, which is devoted to both creation and support of the close collaboration between citizens and institutions in the Visegrad Group countries as well as the cooperation of these countries with other states and regions.
- Klíčová slova
- metalomika,
- MeSH
- bioanorganická chemie * MeSH
- biomedicínský výzkum MeSH
- kovy MeSH
- lékařská onkologie MeSH
- lidé MeSH
- metalothionein MeSH
- mezinárodní spolupráce * MeSH
- nanotechnologie * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- úvodní články MeSH
- zprávy MeSH
Laboratory of metallomics and nanotechnologies attended the important event of traditional World cancer day (WCD) which was held on 4. February 2015. On this occasion were prepared a series of lectures aimed for introduction of nanotechnology research as an improvement for cancer diagnosis in early stages, treatment and progressive ways of healing the oncologic patients by gene therapy or rapid cancer diagnosis.
Alzheimer's disease (AD) is a progressive neurodegenerative disorder affecting tens of million people. Currently marketed drugs have limited therapeutic efficacy and only slowing down the neurodegenerative process. Interestingly, it has been suggested that biometal cations in the amyloid beta (Aβ) aggregate deposits contribute to neurotoxicity and degenerative changes in AD. Thus, chelation therapy could represent novel mode of therapeutic intervention. Here we describe the features of chelators with therapeutically relevant mechanism of action. We have found that the tested compounds effectively reduce the toxicity of exogenous Aβ and suppress its endogenous production as well as decrease oxidative stress. Cholyl hydrazones were found to be the most active compounds. In summary, our data show that cation complexation, together with improving transport efficacy may represent basis for eventual treatment strategy in AD.
- MeSH
- Alzheimerova nemoc farmakoterapie metabolismus MeSH
- amyloidní beta-protein antagonisté a inhibitory metabolismus MeSH
- chelátory chemická syntéza chemie farmakologie MeSH
- chelátová terapie * MeSH
- cholinesterasové inhibitory chemická syntéza chemie farmakologie MeSH
- kationty chemie farmakologie MeSH
- kovy chemie farmakologie MeSH
- lidé MeSH
- molekulární struktura MeSH
- nádorové buňky kultivované MeSH
- proliferace buněk účinky léků MeSH
- proteinové agregáty účinky léků MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In 2012, in the Laboratory of Metallomics and Nanotechnologies has began to solve the European project CZ.1.07/2.3.00/20.0148 „International cooperation in the field of “in vivo” imaging techniques (NANOLABSYS)“. LADYS system was created in addressing to this project. LADYS enabled the effective, uniform and centralized data management, facilitate and accelerate the transfer of new information to members of user networks and in administrative mode to simplify the gathering of information on workers, their daily activities and outputs that the project drew up.
Metallomics is an area that studies the interactions of metal ions with proteins and other biomolecules and their function in living organisms. Metallothioneins (MTs) belong to a large family of metalloproteins in this field. As it is known, the presence of Cd2+ ions causes higher expression of MTs. Therefore we focused on determination of MT and Cd content in liver from chicken embryos (model organism) by electrochemistry and atomic absorption spectroscopy (AAS) after the exposure of chicken embryos to different concentrations of Cd(NO3)2. We also determined the spatial distribution of MT in chicken liver tissue slices by matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) and evaluated the toxicity of Cd(NO3)2 to chicken embryos. Finally, we attempted to find the correlation between 2D MSI maps of MT and concentration of MT in chicken liver.
- MeSH
- elektrochemické techniky MeSH
- játra * chirurgie metabolismus účinky léků MeSH
- kadmium * metabolismus toxicita MeSH
- kuřecí embryo MeSH
- metalothionein * chemie metabolismus MeSH
- organokovové sloučeniny toxicita MeSH
- spektrofotometrie atomová MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
This work studied the mechanism of action of a Pt(IV) complex 2 bearing two axial lonidamine ligands, which are selective inhibitors of aerobic glycolysis. The presence of two lonidamine ligands in 2 compared to the parent Pt(II) complex increased its antiproliferative activity, cellular accumulation, and changed its cell cycle profile and mechanism of cell death. In 3D cell culture, 2 showed exceptional antiproliferative activity with IC50 values as low as 1.6 μM in MCF7 cells. The study on the influence of the lonidamine ligands in the Pt complex on glycolysis showed only low potency of ligands to affect metabolic processes in cancer cells, making the investigated complex, not a dual- or multi-action prodrug. However, the Pt(IV) prodrug effectively delivers the cytotoxic Pt(II) complex into cancer cells.
Aerobic organisms require oxygen for respiration but must simultaneously cope with oxidative damages inherently linked with this molecule. Unicellular amoeboflagellates of the genus Naegleria, containing both free-living species and opportunistic parasites, thrive in aerobic environments. However, they are also known to maintain typical features of anaerobic organisms. Here, we describe the mechanisms of oxidative damage mitigation in Naegleria gruberi and focus on the molecular characteristics of three noncanonical proteins interacting with oxygen and its derived reactive forms. We show that this protist expresses hemerythrin, protoglobin, and an aerobic-type rubrerythrin, with spectral properties characteristic of the cofactors they bind. We provide evidence that protoglobin and hemerythrin interact with oxygen in vitro and confirm the mitochondrial localization of rubrerythrin by immunolabeling. Our proteomic analysis and immunoblotting following heavy metal treatment revealed upregulation of hemerythrin, while rotenone treatment resulted in an increase in rubrerythrin protein levels together with a vast upregulation of alternative oxidase. Our study provided new insights into the mechanisms employed by N. gruberi to cope with different types of oxidative stress and allowed us to propose specific roles for three unique and understudied proteins: hemerythrin, protoglobin, and rubrerythrin.
