Černucha setá (Nigella sativa l.) je jednoletá bylina z čeledi pryskyřníkovitých, původní v západní asii a severní africe. Její semena se používají jako koření, zejména v Indii a na Středním východě, ale také k léčebným účelům. Semena černuchy obsahují velké množství bioaktivních látek, které vykazují množství farmakologických účinků. Černucha setá patří mezi donedávna zapomenuté léčivky a v různých systémech tradiční medicíny se používá již tisíce let, ale studie moderní medicíny posledních let ukazují, že její léčebné využití může být mnohem širší. Je pravděpodobné, že by mohla najít využití i v léčbě psychických poruch, jako jsou úzkost, deprese a některá neurodegenerativní onemocnění. Bez zajímavosti není ani její potenciální využití v terapii covidu-19.
Nigella sativa is an annual herb of the buttercup family, native to Western Asia and North Africa. Its seeds are used as a spice, especially in india and the Middle east, but also for medicinal purposes. Nigella sativa seeds contain a large number of bioactive substances, which have a number of pharmacological effects. It is a forgotten medicine and has been used in various systems of traditional medicine for thousands of years, but studies of modern medicine in recent years show that its therapeutic use can be much wider. it is likely that it could also find use in the treatment of mental disorders such as anxiety, depression and some neurodegenerative diseases. Its potential use in COVID-19 therapy is not without interest.
- MeSH
- Phytotherapy methods MeSH
- Plants, Medicinal MeSH
- Humans MeSH
- Nervous System drug effects MeSH
- Nigella sativa * MeSH
- Plant Extracts therapeutic use MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Černucha setá (Nigella sativa L.) je jednoletá bylina z čeledi pryskyřníkovitých, původní v západní Asii a severní Africe. Její semena se používají jako koření, zejména v Indii a na Středním východě, ale také k léčebným účelům. Semena černuchy obsahují velké množství bioaktivních látek, které vykazují množství farmakologických účinků. Černucha setá patří mezi donedávna zapomenuté léčivky a v různých systémech tradiční medicíny se používá již tisíce let, ale studie moderní medicíny posledních let ukazují, že její léčebné využití může být mnohem širší. Je pravděpodobné, že by mohla najít využití i v léčbě psychických poruch, jako jsou úzkost, deprese a některá neurodegenerativní onemocnění. Bez zajímavosti není ani její potenciální využití v terapii covidu-19.
Nigella sativa is an annual herb of the buttercup family, native to Western Asia and North Africa. Its seeds are used as a spice, especially in India and the Middle East, but also for medicinal purposes. Nigella sativa seeds contain a large number of bioactive substances, which have a number of pharmacological effects. It is a forgotten medicine and has been used in various systems of traditional medicine for thousands of years, but studies of modern medicine in recent years show that its therapeutic use can be much wider. It is likely that it could also find use in the treatment of mental disorders such as anxiety, depression and some neurodegenerative diseases. Its potential use in COVID-19 therapy is not without interest.
- MeSH
- Phytotherapy methods MeSH
- Plants, Medicinal * MeSH
- Humans MeSH
- Nigella sativa * MeSH
- Check Tag
- Humans MeSH
In this study, the antioxidant capacities of main quinone constituents of Nigella sativa seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 microg/mL and IC50 value of 2.4 microg/mL in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 microg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties.
- MeSH
- Antioxidants pharmacology MeSH
- Benzoquinones pharmacology MeSH
- Quinones pharmacology MeSH
- Nigella sativa chemistry MeSH
- Free Radical Scavengers pharmacology MeSH
- Seeds chemistry MeSH
- Thymol analogs & derivatives pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
INTRODUCTION: Thymoquinone (TQ) is one of the bioactive compounds in Nigella sativa (NS). Also known as black seeds/cumin, it has been postulated to possess anti-atherogenic properties. However, research on the effects of NS oil (NSO) and TQ on atherogenesis remain scarce. The aim of this study is to determine gene and protein expression of Intercellular Adhesion Molecule-1 (ICAM-1), Vascular Cell Adhesion Molecule-1 (VCAM-1), and Endothelial-eukocyte adhesion molecule (E-selectin) in Human Coronary Artery Endothelial Cells (HCAECs). METHODS: HCAECs were stimulated for 24 hours (h) with 200 μg/ml of Lipopolysaccharides (LPS) and different concentrations of NSO (55, 110, 220, 440 μg/ml) or TQ (4.5, 9.0, 18.0, 36.0 μm). The effects of NSO and TQ on gene and protein expressions were measured using multiplex gene assay and ELISA assay, respectively. Rose Bengal assay was used to analyse monocyte binding activity. RESULTS: NSO and TQ significantly reduced ICAM-1 and VCAM-1 gene and protein expressions. TQ showed significant reduction activity of the biomarkers in dose dependent manner. HCAECs pre-treated with NSO and TQ for 24 h significantly lowered monocytes adherence compared to non-treated HCAECs. CONCLUSIONS: NSO and TQ supplementation have anti-atherogenic properties and inhibit monocytes' adherence to HCAECs via down-regulation of ICAM-1 expression. NSO could potentially be incorporated in standard treatment regimens to prevent atherosclerosis and its related complications.
- MeSH
- Vascular Cell Adhesion Molecule-1 genetics pharmacology MeSH
- Endothelial Cells MeSH
- Humans MeSH
- Intercellular Adhesion Molecule-1 genetics pharmacology MeSH
- Monocytes * MeSH
- Nigella sativa * chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In 2015, the Nobel Prize in Physiology or Medicine was awarded to Youyou Tu, for her discoveryof the natural anti-malarial drug Artemisinin [...].
- MeSH
- Antimalarials * MeSH
- Diabetes Mellitus, Type 2 * MeSH
- Humans MeSH
- Nigella sativa * MeSH
- Nobel Prize MeSH
- Awards and Prizes * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Comment MeSH
- MeSH
- Quinones administration & dosage pharmacology therapeutic use MeSH
- Prostaglandin-Endoperoxide Synthases metabolism MeSH
- Dinoprostone biosynthesis MeSH
- Research Support as Topic MeSH
- Phytotherapy MeSH
- Inhibitory Concentration 50 MeSH
- Cyclooxygenase Inhibitors administration & dosage pharmacology therapeutic use MeSH
- Humans MeSH
- Membrane Proteins MeSH
- Nigella sativa MeSH
- Plant Extracts administration & dosage pharmacology therapeutic use MeSH
- Thymol administration & dosage pharmacology therapeutic use MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
Seed extracts from six species of the genus Nigella (Family Ranunculaceae)-Nigella arvensis, Nigella damascena, Nigella hispanica, Nigella nigellastrum, Nigella orientalis, and Nigella sativa-obtained by successive extraction with n-hexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution method as well as for anti-inflammatory properties by in vitro cyclooxygenase (COX)-1 and COX-2 assay. Chemical characterization of active extracts was carried out including free and fixed fatty acid analysis. Comparison of antimicrobial activity showed that N. arvensis chloroform extract was the most potent among all species tested, inhibiting Gram-positive bacterial and yeast strains with minimum inhibitory concentration (MIC) values ranging from 0.25 to 1 mg/mL. With the exception of selective inhibitory action of n-hexane extract of N. orientalis on growth of Bacteroides fragilis (MIC = 0.5 mg/mL), we observed no antimicrobial activity for other Nigella species. Anti-inflammatory screening revealed that N. sativa, N. orientalis, N. hispanica, N. arvensis n-hexane, and N. hispanica chloroform extracts had strong inhibitory activity (more than 80%) on COX-1 and N. orientalis, N. arvensis, and N. hispanica n-hexane extracts were most effective against COX-2, when the concentration of extracts was 100 microg/mL in both COX assays. In conclusion, N. arvensis, N. orientalis, and N. hispanica seeds, for the first time examined for antimicrobial and anti-inflammatory effects, revealed their significant activity in one or both assays.
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Anti-Inflammatory Agents pharmacology MeSH
- Antifungal Agents pharmacology MeSH
- Bacteria drug effects MeSH
- Candida albicans drug effects MeSH
- Cyclooxygenase 1 metabolism MeSH
- Cyclooxygenase 2 metabolism MeSH
- Microbial Sensitivity Tests MeSH
- Nigella chemistry MeSH
- Plant Extracts pharmacology MeSH
- Seeds MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Plants, Medicinal MeSH
- Humans MeSH
- Plant Extracts pharmacology therapeutic use MeSH
- Seeds MeSH
- Check Tag
- Humans MeSH
