Q63368351
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Nogo-A protein is an important inhibitor of axonal growth, which also regulates neuronal plasticity in the CNS. Mutations in the gene encoding Nogo-A or abnormalities in Nogo-A expression are linked to neuropsychiatric disorders such as schizophrenia. The present study assesses the impact of constitutively reduced expression of Nogo-A on place navigation in a novel transgenic rat model. Two spatial paradigms were used: (1) A battery of tests in the Carousel maze requiring continuous processing of spatial information with increasing demands for the segregation of reference frames and behavioral flexibility and (2) a delayed-matching-to-place version of the Morris water maze (MWM), which requires place navigation and is sensitive to deficits in one-trial-encoded place representation. The Carousel maze testing revealed a subtle but significant impairment in management of reference frames. Matching-to-place learning in the Morris water maze was unaffected, suggesting an intact representation of an unmarked goal. Our results show that Nogo-A deficiency leads to cognitive deficit in processing of the reference frames. Such a deficit may be the result of neuro-developmental alterations resulting from Nogo-A deficiency.
- MeSH
- bludiště - učení fyziologie MeSH
- down regulace * MeSH
- genový knockdown MeSH
- krysa rodu rattus MeSH
- myelinové proteiny genetika metabolismus MeSH
- potkani Sprague-Dawley MeSH
- potkani transgenní MeSH
- prostorové chování fyziologie MeSH
- učení vyhýbat se fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Spatial navigation comprises a widely-studied complex of animal behaviors. Its study offers many methodological advantages over other approaches, enabling assessment of a variety of experimental questions and the possibility to compare the results across different species. Spatial navigation in laboratory animals is often considered a model of higher human cognitive functions including declarative memory. Almost fifteen years ago, a novel dry-arena task for rodents was designed in our laboratory, originally named the place avoidance task, and later a modification of this approach was established and called active place avoidance task. It employs a continuously rotating arena, upon which animals are trained to avoid a stable sector defined according to room-frame coordinates. This review describes the development of the place avoidance tasks, evaluates the cognitive processes associated with performance and explores the application of place avoidance in the testing of spatial learning after neuropharmacological, lesion and other experimental manipulations.
- MeSH
- behaviorální výzkum metody MeSH
- chování zvířat fyziologie MeSH
- druhová specificita MeSH
- kognice fyziologie MeSH
- modely u zvířat MeSH
- neurovědy metody MeSH
- paměť fyziologie MeSH
- prostorové chování fyziologie MeSH
- učení vyhýbat se fyziologie MeSH
- vnímání prostoru fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
The involvement of various neurotransmitter receptors in the brain in the regulation of spatial behavior is a focus of interest for many cognitive neuroscientists. Active allothetic place avoidance (AAPA) task have been demonstrated to require spatial mapping and cognitive coordination and is highly dependent upon hippocampus. The present study was designed to evaluate the role of beta-adrenergic receptors in the modulation of locomotor and spatial behavior in this task. Four doses of centrally active beta-adrenergic antagonist propranolol (5, 20, 25 and 30 mg/kg) were administered intraperitoneally 30 min prior to testing in the place avoidance task. Four daily sessions were pursued, each lasting 20 min. A dose of 25 mg/kg was found to induce a deficit in spatial behavior (measured by number of entrances into the shock sector) without altering locomotion; lower doses were without effect. The highest dose (30 mg/kg) impaired both locomotion and avoidance behavior. The results suggest that beta-adrenoceptors are involved in the regulation of behavior in the place avoidance task and that it is possible to dissociate the effect of propranolol on the spatial performance and locomotion in the AAPA using dose-selection.
- MeSH
- analýza rozptylu MeSH
- beta blokátory farmakologie MeSH
- chování zvířat účinky léků MeSH
- financování organizované MeSH
- kognice fyziologie účinky léků MeSH
- krysa rodu rattus MeSH
- pohybová aktivita účinky léků MeSH
- potkani Long-Evans MeSH
- propranolol farmakologie MeSH
- prostorové chování účinky léků MeSH
- rozvrh dávkování léků MeSH
- učení vyhýbat se účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
The processing of spatial information is the focus of interest for many cognitive neuroscientists. Approximately 10 years ago, a novel behavioral paradigm called active allothetic place avoidance (AAPA) was designed allowing the simultaneous assessment of locomotor and spatial behavior. The present study describes the effect of the combined treatment of Long-Evans rats with alpha1-adrenergic and D2 antagonists prazosin (1mg/kg and 2 mg/kg) and sulpiride (10 mg/kg and 30 mg/kg) on locomotion and avoidance behavior in the AAPA task. Results show that co-application of both drugs leads to disturbances in locomotion and avoidance in rats at the doses, which caused no impairments when administered independently. This finding suggests that both types of receptors act synergistically to regulate locomotion and possibly spatial behavior.
- MeSH
- alfa blokátory aplikace a dávkování MeSH
- alfa-1-adrenergní receptory metabolismus účinky léků MeSH
- analýza rozptylu MeSH
- antagonisté dopaminu aplikace a dávkování MeSH
- asociační učení fyziologie účinky léků MeSH
- financování organizované MeSH
- interakce mezi receptory a ligandy fyziologie MeSH
- krysa rodu rattus MeSH
- pohybová aktivita fyziologie účinky léků MeSH
- potkani Long-Evans MeSH
- prazosin aplikace a dávkování MeSH
- prostorové chování fyziologie účinky léků fyziologie MeSH
- receptory dopaminu D2 metabolismus účinky léků MeSH
- sulpirid aplikace a dávkování MeSH
- synergismus léků MeSH
- učení vyhýbat se fyziologie účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- životní prostředí MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- Alzheimerova nemoc * etiologie patofyziologie terapie MeSH
- farmakoterapie metody MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
