Q80407527
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BACKGROUND DATA AND OBJECTIVES: Nd:YAG laser radiation has been applied in treatment of dentine hypersensitivity; dentine melt created after irradiation on a tooth surface can occlude open dentinal tubules. Different conditions (energy of pulses, number of successive irradiations, and type of dye agent) have been reported in the literature. In our study, different conditions are compared and the most suitable ones are suggested. METHODS: One hundred nine dentine samples prepared from extracted human third molars in the form of 2- or 3-mm thick discs and facets from an outer convex surface of a root were irradiated by pulses with duration 0.3 ms and beam diameter 0.3 or 0.6 mm. The laser repetition rate was 15 Hz. Energies per pulse have been changed from 20 mJ to 250 mJ (the corresponding energy densities rho are 22 and 275 J/cm(2) for the beam diameter of 0.3 mm). Dyes including tartrazine, carmine indigo, erythrosin, and methylene blue have been used to cover dentine surfaces and to increase absorption of laser radiation. Irradiated dentine surfaces have been investigated using scanning electron microscopy. RESULTS: Sealing of open dentinal tubules can be accomplished even without dyes, provided that a sufficiently intense laser radiation level is used. To avoid damage to the dentine's surface the use of dyes is necessary. Erythrosin has been found to be the best agent, and closing of dentinal tubules occurred after four doses of irradiation with 30-mJ pulses with a beam diameter of 0.6 mm (energy density rho = 8.25 J/cm(2), total energy density rho(T) = 33 J/cm(2)). The upper limit has been reached using four doses of irradiation by 90-mJ pulses with a beam diameter of 0.3 mm (rho = 99 J/cm(2), rho(T) = 396 J/cm(2)). CONCLUSIONS: Occluding dentinal tubules can safely and effectively be accomplished using pulsed Nd:YAG laser radiation provided that the dentine surface is covered with erythrosin solution.
- MeSH
- citlivost dentinu prevence a kontrola MeSH
- dentin účinky záření ultrastruktura MeSH
- erythrosin aplikace a dávkování terapeutické užití MeSH
- financování organizované MeSH
- fluorescenční barviva aplikace a dávkování terapeutické užití MeSH
- laserová terapie MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- neodym MeSH
- permeabilita dentinu účinky záření MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- barvicí látky chemie terapeutické užití MeSH
- citlivost dentinu etiologie terapie MeSH
- dentin chemie účinky záření ultrastruktura MeSH
- erythrosin MeSH
- extrakce zubů MeSH
- finanční podpora výzkumu jako téma MeSH
- laserová koagulace metody využití MeSH
- laserová terapie MeSH
- lasery MeSH
- lidé MeSH
- molár třetí účinky záření MeSH
- Check Tag
- lidé MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
64 l. : il., tab. ; 30 cm
Stanovení fyzikálních charakteristik Nd:YAG laseru pro modifikaci povrchové morfologie exponovaného dentinu lidských extrahovaných molárů ve smyslu tubulové okluze. Vyhodnocení povrchové morfologie exponovaného dentinu ozářeného a neozářeného Nd:YAG laserem pomocí SEM (Scanning Electron Microscopy) a AFM (Atomic Force Microscopy). Zhodnocení přínosu AFM pro vyšetření morfologie exponovaného dentinu lidských extrahovaných zubů.; Determination of characteristics of Nd:YAG laser irradiation for the occluding of exposed dentinal tubules of extracted human molars. Characterisation of surface morphology of exposed dentin both irradiated and not irradiated by Nd:YAG laser by ScanningElectron Microscopy (SEM) and Atomic Force Microscopy (AFM). Determination of the usefulness of Atomic-force microscopy to the characterization of morphology of exposed dentin of extracted human teeth.
