Wolffian duct Dotaz Zobrazit nápovědu
Oxidative stress is a possible source of spermatozoa function deterioration. Seminal fluid (SF) protects spermatozoa against reactive oxygen species (ROS) attack during development in testes and transit through the reproductive tract. Spermatozoa curvilinear velocity and percent of motile cells as well as changes in thiobarbituric acid-reactive substance (TBARS) content, superoxide dismutase, and catalase activity, and uric acid concentration in SF were evaluated in sterlet sperm collected from testes 24 h after hormone induction of spermiation and from Wolffian ducts at 12, 24, 36, and 60 h after hormone injection (HI). While testicular spermatozoa motility was not initiated in activating medium, Wolffian duct sperm showed low motility at 12 h, significant increase at 24 and 36 h, and decrease at 60 h. Testicular SF was characterized by the highest level of TBARS and activity of studied enzymes compared with SF from Wolffian duct sperm at 24 h post-HI. In fluid from Wolffian duct sperm, a significant increase in TBARS content was shown at 36-60 h post-HI. In contrast to testicular SF, in SF from Wolffian duct sperm, this increase was not counterbalanced by changes in the studied variables of antioxidant system. This may be the source of the observed decrease in spermatozoa motility parameters 60 h post-HI. The results may confirm a dual role of ROS in fish sperm physiology. The data with respect to decrease in sturgeon spermatozoa motility parameters at 60 h post-HI should be taken into account in artificial sturgeon propagation.
- MeSH
- antioxidancia metabolismus MeSH
- kyselina močová chemie metabolismus MeSH
- látky reagující s kyselinou thiobarbiturovou chemie metabolismus MeSH
- počet spermií MeSH
- ryby fyziologie MeSH
- sperma metabolismus MeSH
- spermie cytologie fyziologie MeSH
- Wolffovy vývody metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. There are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role is recognized in mammalian sperm maturation. The present study evaluated the involvement of seminal fluid proteases and anti-proteolytic activity in the sterlet spermatozoon maturation process. Casein and gelatin zymography and quantification of amidase and anti-proteolytic activity were conducted in sturgeon seminal fluid from Wolffian duct sperm and seminal fluid from testicular sperm, along with spermatozoon extracts from Wolffian duct spermatozoa, testicular spermatozoa, and testicular spermatozoa after in vitro maturation. We did not find significant differences in proteolytic profiles of seminal fluids from Wolffian duct sperm and ones from testicular sperm. Zymography revealed differences in spermatozoon extracts: Wolffian duct spermatozoon extracts were characterized by the presence of a broad proteolytic band ranging from 48 to 41 kDa, while testicular spermatozoon extracts did not show such activity until after in vitro maturation. The differences in amidase activity coincided with these results. It may not be the levels of proteolytic and anti-proteolytic activity per se, but the alterations in their interactions triggering a cascade of signaling events, that is crucial to the maturation process.
- MeSH
- amidohydrolasy metabolismus MeSH
- motilita spermií MeSH
- proteolýza MeSH
- ryby fyziologie MeSH
- spermie fyziologie MeSH
- testis cytologie MeSH
- Wolffovy vývody cytologie MeSH
- zrání spermie * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- diferenciální diagnóza MeSH
- lidé MeSH
- nemoci děložních adnex diagnóza patologie MeSH
- Wolffovy vývody embryologie patologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5min at 20°C and were designated 'TS after IVM' (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5min at 20°C) in the presence of 2mM EGTA, 100µM Verapamil or 100µM Tetracaine. No motility was observed in the case of TS (10mM Tris, 25mM NaCl, 50mM Sucr with or without the addition of 2mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1-2nM for Wolffian duct spermatozoa and TSM; approximately 0.6mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.
- MeSH
- blokátory kalciových kanálů farmakologie MeSH
- iontový transport MeSH
- kultivační média MeSH
- motilita spermií účinky léků fyziologie MeSH
- ryby metabolismus MeSH
- sperma metabolismus MeSH
- spermie účinky léků metabolismus MeSH
- techniky in vitro MeSH
- testis cytologie MeSH
- vápník metabolismus farmakologie MeSH
- verapamil farmakologie MeSH
- Wolffovy vývody metabolismus MeSH
- zrání spermie účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Herlyn-Werner-Wunderlichov syndróm je vzácna kongenitálna anomália, definovaná triádou vrodených chýb ženského urogenitálneho traktu – homolaterálnou renálnou agenéziou, uterom duplex a obštrukčným hemihematometrokolpom, ktorej etiológia zostáva zatiaľ nejasná. Najčastejšími klinickými príznakmi sú panvová bolesť, dysmenorrhea a hmatateľná hmota v dôsledku hematokolpu alebo hematometra. Vzhľadom na nedávne štúdie sa u týchto mladých pacientok považuje za dominantný nález endometrióza, čo môže vysvetľovať patofyziologický mechanizmus jej vzniku ako výsledok retrográdnej menštruácie. Včasná diagnostika a následná liečba sú dôležité v prevencii rozvoja závažných komplikácií. Klinické a reprodukčné výsledky po drenáži hematometrokolpu sú vo všeobecnosti uspokojivé. Predstavujeme 13-ročnú pacientku s panvovou bolesťou a diagnostikovaným syndrómom OHVIRA liečenú balónikovou septostómiou.
Herlyn-Werner-Wunderlich syndrome is an unusual congenital anomaly defined by a triad of congenital defects of the female urogenital tract – homolateral renal agenesis, uterus duplex and obstructed hemi-hematometrocolpos whose etiology remains still unclear. Pelvic pain, dysmenorrhea and palpable mass due to the hematocolpos or hematometra are the most common clinical symptoms. Endometriosis is considered to be a prevalent finding in these young patients possibly explaining the pathophysiological mechanism of endometriosis as the result of retrograde menstruation. Early diagnosis and subsequent treatment are important to prevent the development of severe complications. In general, clinical and reproductive outcomes after drainage of the hematometrocolpos are reported to be satisfactory. A 13-year-old patient presented with pelvic pain and was diagnosed with OHVIRA syndrome treated with Balloon septostomy.
- Klíčová slova
- Herlyn-Werner-Wunderlichův syndrom, septosomie,
- MeSH
- dítě MeSH
- ductus Mülleri chirurgie patologie MeSH
- gynekologické chirurgické výkony * metody přístrojové vybavení MeSH
- katetrizace metody MeSH
- lidé MeSH
- ultrasonografie MeSH
- urogenitální abnormality * chirurgie diagnostické zobrazování patologie MeSH
- uterus duplex diagnostické zobrazování patologie MeSH
- Wolffovy vývody chirurgie patologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- Klíčová slova
- Herlyn-Werner-Wunderlichův syndrom,
- MeSH
- časná diagnóza MeSH
- ductus Mülleri abnormality diagnostické zobrazování patologie MeSH
- lidé MeSH
- mladiství MeSH
- mnohočetné abnormality chirurgie diagnostické zobrazování patologie MeSH
- ovariální cysty chirurgie diagnostické zobrazování MeSH
- solitární ledvina diagnostické zobrazování MeSH
- syndrom MeSH
- urogenitální abnormality * chirurgie diagnostické zobrazování patologie MeSH
- uterus chirurgie diagnostické zobrazování patologie MeSH
- vagina abnormality chirurgie diagnostické zobrazování MeSH
- Wolffovy vývody abnormality diagnostické zobrazování patologie MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- práce podpořená grantem MeSH
Congenital developmental defects of Müllerian derivates, understandable with the knowledge of embryological development of Wolffian and Müllerian ducts, are defects of canalisation (= gynatresias), defects in fusing, combined defects and uterovaginal agenesis. Gynatresias should be suspected in the newborn, but distinguished in puberty, on the basis of menstrual blood retention, as hymeneal atresia (haematocolpos), aplasia partis distalis vaginae (haematocolpos partialis), transversal vagina septum and aplasia of vagina and uterine cervix (isolated haematometra). Particular operations are described. Defects in the fusing of Müllerian ducts from the point of view of surgery could be single-coated or double-coated. Incomplete reduplication with unilateral renal aplasia syndrome could present as hemihaematocolpos, hemihaematometra and haematometra in rudimental horn. For diagnosis of these disorders menstrual blood retention is necessary. Surgical treatment in the first two types includes resection of the common wall and haematometra in rudimental horn needs metroplasty or hemihysterectomy. Congenital absence of uterus and vagina (Rokitanski Küster) appears in genetically, endocrinologically and psychosexually normal females. Diagnosis is based on clinical examination and ultrasonography. Initial examination should be nonsurgical (dilatation method). All surgical corrections create a place for the future vagina and reach its epithelization. Different procedures are criticised. The author recommends and describes Vecchietti's laparoscopic surgery.
- MeSH
- biologické modely MeSH
- ductus Mülleri abnormality chirurgie MeSH
- ledviny abnormality chirurgie MeSH
- lidé MeSH
- mnohočetné abnormality diagnóza chirurgie terapie MeSH
- novorozenec MeSH
- páteř abnormality chirurgie MeSH
- směrnice pro lékařskou praxi jako téma MeSH
- somity abnormality chirurgie MeSH
- urogenitální abnormality diagnóza chirurgie terapie MeSH
- uterus abnormality chirurgie MeSH
- vagina abnormality chirurgie MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
