• MeSH
• biologická dostupnost MeSH
• léky s prodlouženým účinkem analýza   MeSH
• tablety analýza   MeSH
• způsoby aplikace léků MeSH

Flow-through cell dissolution method was implemented in pharmacopoeias in 1990 and since that time its usage significantly increased. It is employed in oral dosage forms with controlled drug release to measure dissolved drug amount within particular time intervals and to characterize pure drug properties (apparent dissolution). Modified flow-through cell is used to determine the drug dissolution from lipophilic dosage forms such as suppositories and soft capsules. Recently, few modifications of this method were published in order to provide drug release studies from parenteral controlled release products and inhalation dosage forms. Review article is presenting last innovations in this area, i.e. new adjustment and arrangement of flow-through cell method, to assess drug release from implants, micro- and nanoparticulate systems, stents and inhalation medicaments in the way more corresponding to in-vivo conditions.

• Klíčová slova
• in vitro - in vivo,
• MeSH
• aplikace inhalační MeSH
• biologická dostupnost MeSH
• elektrochemické techniky * metody   přístrojové vybavení   využití   MeSH
• farmaceutická technologie normy   MeSH
• hodnocení léčiv * MeSH
• lékové formy normy   MeSH
• lékové transportní systémy * MeSH
• lidé MeSH
• nanočástice MeSH
• protézy a implantáty MeSH
• referenční knihy normy   MeSH
• rozpustnost * MeSH
• stenty MeSH
• Check Tag
• lidé MeSH

• MeSH
• farmakokinetika MeSH
• kongresy jako téma MeSH
• lékové formy MeSH

Purpose: Due to the shortcomings of nanocarriers, the development of carrier-free nanodelivery systems has attracted more and more attention in cancer treatment. However, there are few studies on carrier-free nanosystems that can simultaneously achieve monitoring functions. Here a multifunctional carrier-free nanosystem loaded with curcumin and irinotecan hydrochloride was established for the treatment and monitoring of gastric cancer. Methods: In this study, an irinotecan hydrochloride-curcumin nanosystem in the early stage (the system is named SICN) was prepared. Based on the fluorescence of curcumin, flow cytometry, laser confocal microscopy, and zebrafish fluorescence imaging were used to study the monitoring function of SICN in vivo and in vitro. In addition, HGC-27 human gastric cancer cells were used to study SICN cytotoxicity. Results: Flow cytometry and zebrafish fluorescence imaging monitoring results showed that the uptake of SICN was significantly higher than free curcumin, and the excretion rate was lower. SICN had higher accumulation and retention in cells and zebrafish. Laser confocal microscopy monitoring results showed that SICN was internalized into HGC-27 cells through multiple pathways, including macropinocytosis, caveolin, and clathrin-mediated and clathrin -independent endocytosis, and distributed intracellularly throughout the whole cytoplasm, including lysosomes and Golgi apparatus. In vitro cell experiments showed that SICN nanoparticles were more toxic than single components, and HGC-27 cells had more absorption and higher toxicity to nanoparticles under slightly acidic conditions. Conclusion: SICN is a promising carrier-free nanoparticle, and the combination of two single-component therapies can exert a synergistic antitumor effect. When exposed to a tumor acidic environment, SICN showed stronger cytotoxicity due to charge conversion. More importantly, the nanoparticles' self-monitoring function has been developed, opening up new ideas for combined tumor therapy.

• MeSH
• apoptóza účinky léků   MeSH
• buněčná smrt účinky léků   MeSH
• buněčný cyklus účinky léků   MeSH
• dánio pruhované MeSH
• endocytóza účinky léků   MeSH
• fluorescence MeSH
• irinotekan farmakologie   terapeutické užití   MeSH
• kurkumin aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• lékové transportní systémy * MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• nádory farmakoterapie   MeSH
• nanočástice chemie   MeSH
• nosiče léků MeSH
• uvolňování léčiv MeSH
• velikost částic MeSH
• zobrazování trojrozměrné MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• anatomie MeSH
• fyziologie MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Fyziologie člověka a srovnávací fyziologie
• NLK Obory
• fyziologie
• anatomie