reference library
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Příspěvek shrnuje výsledky kvantitativního průzkumu provedeného mezi studenty a pracovníky 2. lékařské fakulty Univerzity Karlovy a Fakultní nemocnice v Motole. Hlavním cílem je podat přehled o jejich informačním chování a zjistit informovanost o tzv. nadstavbových službách, které jsou v rámci knihovny nabízeny. Jedná se převážně o služby referenční, rešeršní, meziknihovní, vzdělávací, propagační. Příspěvek srovnává informační chování jednotlivých uživatelů, využívání nadstavbových služeb, používání sociálních sítí, hodnocení výuky informační gramotnosti. Závěrečná část průzkumu, tentokrát kvalitativního charakteru, shrnuje náměty a připomínky uživatelů k jednotlivým činnostem knihovny.
This paper summarizes outcomes of quantitative research that has been made among students and staff of 2nd Faculty of Medicine at Charles University and University Hospital in Motol. The main goal is to give an overview of their information behaviour as well as to find out how the users are informed about superstructural services (they are reference, search, educational, promotional and interlibrary loan services) which are offered by library itself. The paper compares information behaviour of each user, superstructural services use, social sites use, and evaluation of information literacy classes. The final qualitative part of the research sums up users’ suggestions and comments on particular library activity.
Detection of peptides lies at the core of bottom-up proteomics analyses. We examined a Bayesian approach to peptide detection, integrating match-based models (fragments, retention time, isotopic distribution, and precursor mass) and peptide prior probability models under a unified probabilistic framework. To assess the relevance of these models and their various combinations, we employed a complete- and a tail-complete search of a low-precursor-mass synthetic peptide library based on oncogenic KRAS peptides. The fragment match was by far the most informative match-based model, while the retention time match was the only remaining such model with an appreciable impact--increasing correct detections by around 8 %. A peptide prior probability model built from a reference proteome greatly improved the detection over a uniform prior, essentially transforming de novo sequencing into a reference-guided search. The knowledge of a correct sequence tag in advance to peptide-spectrum matching had only a moderate impact on peptide detection unless the tag was long and of high certainty. The approach also derived more precise error rates on the analyzed combinatorial peptide library than those estimated using PeptideProphet and Percolator, showing its potential applicability for the detection of homologous peptides. Although the approach requires further computational developments for routine data analysis, it illustrates the value of peptide prior probabilities and presents a Bayesian approach for their incorporation into peptide detection.
xx, 799 s.
- Klíčová slova
- programovací jazyk C, programovací jazyky,
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- lékařská informatika
Large insert genomic DNA libraries are useful resources for genomic studies. Although the genome of Xenopus tropicalis stands as the amphibian reference genome because it benefitted from large-scale sequencing studies, physical mapping resources such as BAC libraries are lagging behind. Here we present the construction and characterization of a BAC library that covers the whole X. tropicalis genome. We prepared this BAC library from the genomic DNA of X. tropicalis females of the Adiopodoume strain. We characterized BAC clones by screening for specific loci, by chromosomal localization using FISH and by systematic BAC end sequencing. The median insert size is about 110kbp and the library coverage is around six genome equivalents. We obtained a total of 163,787 BAC end sequences with mate pairs for 77,711 BAC clones. We mapped all BAC end sequences to the reference X. tropicalis genome assembly to enable the identification of BAC clones covering specific loci. Overall, this BAC library resource complements the knowledge of the X. tropicalis genome and should further promote its use as a reference genome for developmental biology studies and amphibian comparative genomics.
- MeSH
- genomika metody MeSH
- genová knihovna * MeSH
- hybridizace in situ fluorescenční MeSH
- játra chemie MeSH
- mapování chromozomů MeSH
- proteiny Xenopus genetika MeSH
- sekvenční analýza DNA MeSH
- umělé bakteriální chromozomy genetika MeSH
- Xenopus genetika MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
sv.
- MeSH
- informační služby MeSH
- informační systémy MeSH
- lékařské knihovny MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- lékařská informatika
[1st ed.] 173 s.
- MeSH
- knihovní věda MeSH
- Konspekt
- Knihovnictví. Informatika
- NLK Obory
- knihovnictví, informační věda a muzeologie
Computational exploration of chemical space is crucial in modern cheminformatics research for accelerating the discovery of new biologically active compounds. In this study, we present a detailed analysis of the chemical library of potential glucocorticoid receptor (GR) ligands generated by the molecular generator, Molpher. To generate the targeted GR library and construct the classification models, structures from the ChEMBL database as well as from the internal IMG library, which was experimentally screened for biological activity in the primary luciferase reporter cell assay, were utilized. The composition of the targeted GR ligand library was compared with a reference library that randomly samples chemical space. A random forest model was used to determine the biological activity of ligands, incorporating its applicability domain using conformal prediction. It was demonstrated that the GR library is significantly enriched with GR ligands compared to the random library. Furthermore, a prospective analysis demonstrated that Molpher successfully designed compounds, which were subsequently experimentally confirmed to be active on the GR. A collection of 34 potential new GR ligands was also identified. Moreover, an important contribution of this study is the establishment of a comprehensive workflow for evaluating computationally generated ligands, particularly those with potential activity against targets that are challenging to dock.
- MeSH
- knihovny malých molekul * farmakologie chemie MeSH
- lidé MeSH
- ligandy MeSH
- receptory glukokortikoidů * metabolismus chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
9th ed. xv, 787 s. ; 24 cm
- MeSH
- archivy MeSH
- informatika MeSH
- knihovní věda MeSH
- polygrafie MeSH
- slovníky jako téma MeSH
- Publikační typ
- slovníky MeSH
- Konspekt
- Knihovnictví. Informatika
- NLK Obory
- knihovnictví, informační věda a muzeologie
767 s.
- MeSH
- slovníky jako téma MeSH
- Publikační typ
- slovníky MeSH
- Konspekt
- Všeobecná příručková díla
- NLK Obory
- lingvistika, lékařská terminologie
54th ed. Přeruš. str. : tab. ; 32 cm
