tutorial
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5 diskety + 1 uživatelský manuál (15 s.)
- MeSH
- biochemie MeSH
- Publikační typ
- výukové testy MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
- lékařská informatika
- NLK Publikační typ
- software
Příspěvek se zabývá problematikou šestiměsíčního výukového programu pro začátečníky v karate. Prezentuje výsledky absolvování programu v rámci pohybových dovedností, který je aplikován na vybrané skupině dětí mladšího školního věku. Děti se naučily souboru pohybových dovedností z karate na úrovni 8. kyu a došlo u nich také ke zvýšení fyzické zdatnosti. Obsahem je charakteristika, historie, prostředky karate, specifická metodika výuky technik, která je doplněna o instruktážní DVD. Program vychází z teoretických poznatků a zkušeností trenérů a závodníků v karate. Respektuje a zahrnuje teorii a didaktiku sportovního tréninku, fyziologické zvláštnosti dětí mladšího školního věku a skladbu tréninkové jednotky. Ke zjištění úrovně změn a rozvoje technických dovedností nutných pro složení zkoušky technické vyspělosti je využit zkušební řád Shotokan karate. Zjištěné výsledky výzkumu přinášejí určitá doporučení výuky karate i pro tréninkovou praxi.
The article brings information on the results of the completion of the program for beginners in karate, which is applied to a selected group of primary school children. Children learn to set motor skills of karate at the 8th kyu and will be with them to enhance physical fitness. Its content is characteristic, history, resources karate, specific methodology of teaching techniques, which is supplemented by instructional DVD. The program is based on theoretical knowledge and experience of coaches and athletes in karate respects and includes theoretical and practical knowledge of sports training, physiological peculiarities of children of primary school age and composition of the training unit. To determine the level of change and development of technical skills required to pass the exam technical advance is utilized trial order Shotokan karate. The results of research provide some recommendations for training.
- Klíčová slova
- sportovní trénink,
- MeSH
- bojové sporty * výchova MeSH
- dítě MeSH
- lidé MeSH
- výuka - hodnocení MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
BACKGROUND: Different types of analytical methods, with different characteristics, are applied in metabolomics and lipidomics research and include untargeted, targeted and semi-targeted methods. Ultra High Performance Liquid Chromatography-Mass Spectrometry is one of the most frequently applied measurement instruments in metabolomics because of its ability to detect a large number of water-soluble and lipid metabolites over a wide range of concentrations in short analysis times. Methods applied for the detection and quantification of metabolites differ and can either report a (normalised) peak area or an absolute concentration. AIM OF REVIEW: In this tutorial we aim to (1) define similarities and differences between different analytical approaches applied in metabolomics and (2) define how amounts or absolute concentrations of endogenous metabolites can be determined together with the advantages and limitations of each approach in relation to the accuracy and precision when concentrations are reported. KEY SCIENTIFIC CONCEPTS OF REVIEW: The pre-analysis knowledge of metabolites to be targeted, the requirement for (normalised) peak responses or absolute concentrations to be reported and the number of metabolites to be reported define whether an untargeted, targeted or semi-targeted method is applied. Fully untargeted methods can only provide (normalised) peak responses and fold changes which can be reported even when the structural identity of the metabolite is not known. Targeted methods, where the analytes are known prior to the analysis, can also report fold changes. Semi-targeted methods apply a mix of characteristics of both untargeted and targeted assays. For the reporting of absolute concentrations of metabolites, the analytes are not only predefined but optimized analytical methods should be developed and validated for each analyte so that the accuracy and precision of concentration data collected for biological samples can be reported as fit for purpose and be reviewed by the scientific community.
- MeSH
- hmotnostní spektrometrie * metody MeSH
- lidé MeSH
- metabolomika * metody MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Excessive time for analysis may impede microcirculatory studies with large amounts of video data. Engaging more personnel in the analyses seems to be a rational approach in that scenario and could shorten the time-interval between capturing images and obtaining results. Our hypothesis was that novice users would be able to determine standard microcirculatory parameters using a semi-automated software with an acceptable degree of variability after participating in a standardized interactive training session. 14 volunteers were included in the study. All volunteers analyzed separately the same sample video after the training. The kappa statistic was calculated for the primary outcome parameter microvascular flow index (MFI) within small and large vessels and indicated a fair level of agreement in the results of the novice users. A standardized interactive tutorial can be useful to teach microcirculatory analysis in previously untrained subjects.
- MeSH
- audiovizuální záznam MeSH
- automatizace MeSH
- kardiologie výchova MeSH
- lidé MeSH
- mikrocirkulace * MeSH
- odchylka pozorovatele MeSH
- software MeSH
- statistické modely MeSH
- ústa krevní zásobení MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
- Kanada MeSH
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced single-cell analysis. Single-cell RT-qPCR now represents a well-established method, suitable for an efficient screening prior to single-cell RNA sequencing (scRNA-Seq) experiments, or, oppositely, for validation of hypotheses formulated from high-throughput approaches. Here, we aim to provide a comprehensive summary of the scRT-qPCR method by discussing the limitations of single-cell collection methods, describing the importance of reverse transcription, providing recommendations for the preamplification and primer design, and summarizing essential data processing steps. With the detailed protocol attached in the appendix, this tutorial provides a set of guidelines that allow any researcher to perform scRT-qPCR measurements of the highest standard.
- MeSH
- analýza jednotlivých buněk metody normy MeSH
- kvantitativní polymerázová řetězová reakce metody normy MeSH
- lidé MeSH
- reverzní transkripce genetika MeSH
- senzitivita a specificita MeSH
- stanovení celkové genové exprese metody normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
... Chapter 1 Introduction 1.1 Motivation 1.2 Structure of the tutorial 1.3 Indications for the reader Chapter ...
Vyd. 1. 145 s. : il. ; 30 cm
The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry. It is thus not surprising that the MS instrument attracts the most attention during LC-MS method development, whereas optimizing conditions for peptide separation using reversed-phase liquid chromatography (RPLC) remains somewhat in its shadow. Consequently, the wisdom of the fundaments of chromatography is slowly vanishing from some laboratories. However, the full potential of advanced MS instruments cannot be achieved without highly efficient RPLC. This is impossible to attain without understanding fundamental processes in the chromatographic system and the properties of peptides important for their chromatographic behavior. We wrote this tutorial intending to give practitioners an overview of critical aspects of peptide separation using RPLC to facilitate setting the LC parameters so that they can leverage the full capabilities of their MS instruments. After briefly introducing the gradient separation of peptides, we discuss their properties that affect the quality of LC-MS chromatograms the most. Next, we address the in-column and extra-column broadening. The last section is devoted to key parameters of LC-MS methods. We also extracted trends in practice from recent bottom-up proteomics studies and correlated them with the current knowledge on peptide RPLC separation.
