- MeSH
- Adult MeSH
- Nutritional Physiological Phenomena physiology MeSH
- Carotenoids metabolism MeSH
- Humans MeSH
- Food MeSH
- Food Preferences MeSH
- Aged MeSH
- Skin Aging physiology MeSH
- Aging physiology metabolism MeSH
- Feeding Behavior MeSH
- Vitamin E metabolism MeSH
- Life Style MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Aged MeSH
Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging and skin related damages. In addition, recently achieved progress in nanomedicine opens a novel approach yielding from combination of modern nanotechnology with traditional treatment for innovative pharmacotherapeutics. In the present study, we investigated the antiaging effect of a pretreatment with Myrtus communis natural extract combined with a polycaprolactone nanofibrous scaffold (NanoPCL-M) on skin cell populations exposed to UV. We set up a novel model of skin on a bioreactor mimicking a crosstalk between keratinocytes, stem cells and fibroblasts, as in skin. Beta-galactosidase assay, indicating the amount of senescent cells, and viability assay, revealed that fibroblasts and stem cells pretreated with NanoPCL-M and then exposed to UV are superimposable to control cells, untreated and unexposed to UV damage. On the other hand, cells only exposed to UV stress, without NanoPCL-M pretreatment, exhibited a significantly higher yield of senescent elements. Keratinocyte-based 3D structures appeared disjointed after UV-stress, as compared to NanoPCL-M pretreated samples. Gene expression analysis performed on different senescence associated genes, revealed the activation of a molecular program of rejuvenation in stem cells pretreated with NanoPCL-M and then exposed to UV. Altogether, our results highlight a future translational application of NanoPCL-M to prevent skin aging.
- MeSH
- Gene Expression drug effects MeSH
- Fibroblasts drug effects MeSH
- Keratinocytes drug effects MeSH
- Stem Cells drug effects MeSH
- Cells, Cultured MeSH
- Skin drug effects MeSH
- Humans MeSH
- Myrtus chemistry MeSH
- Nanofibers chemistry MeSH
- Polyesters chemistry MeSH
- Cell Proliferation drug effects MeSH
- Plant Extracts pharmacology MeSH
- Cellular Senescence drug effects MeSH
- Skin Aging drug effects MeSH
- Ultraviolet Rays adverse effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Aktuelle Dermatologie, ISSN 0340-2541 Jahrang 28, Sonderheft 1, Dezember 2002
S23 s. : il., tab., grafy ; 30 cm
- MeSH
- Alopecia MeSH
- Dermatology MeSH
- Skin Diseases MeSH
- Hair Diseases MeSH
- Postmenopause MeSH
- Skin Aging MeSH
- Aging MeSH
- Publication type
- Congress MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- dermatovenerologie
In this study, we have aimed at the preparation and characterization of poly-l-lactic acid (PLLA) composites with antibacterial properties. Thin bilayers of titanium and gold of various thickness ratios were deposited on PLLA by a cathode sputtering method; selected samples were subsequently thermally treated. The surface morphology of the prepared composites was studied by atomic force, scanning electron, and laser confocal microscopy. The chemical properties of the composites were determined by X-ray photoelectron and energy-dispersive X-ray spectroscopy in combination with contact angle and zeta potential analyses. The antibacterial properties of selected samples were examined against a Gram-negative bacterial strain of E. coli. We have found that a certain combination of Au and Ti nanolayers in combination with heat treatment leads to the formation of a unique wrinkled pattern. Moreover, we have developed a simple technique by which a large-scale sample modification can be easily produced. The dimensions of wrinkles can be tailored by the sequence and thickness of the deposited metals. A selected combination of gold, titanium, and heat treatment led to the formation of a nanowrinkled pattern with excellent antibacterial properties.
- Publication type
- Journal Article MeSH
Inflammatory aortic diseases are broadly classified into three categories according to the degree of inflammation: atherosclerosis, atherosclerosis with excessive inflammation, and aortitis/periaortitis. This paper presents a case of a 39-year old man with aneurysmal dilatation of thoracic aorta and aortic valve insufficiency. The aortic wall showed thickening and wrinkled "tree bark" appearance as well as apparent scarring of the intima. Histological examination revealed intimal hyperplasia, a granulomatous/giant cell pattern in the inner tunica media, a few epithelioid macrophages, abundant chronic lymphoplasmacytic and histiocytic inflammation and discrete fibrinoid necrosis. The histological findings were indicative of Horton's disease, but no typical clinical features were present. The case illustrates the difficulties involved in diagnosing inflammatory aortic diseases where it may be challenging to arrive at a specific diagnosis despite the knowledge of medical history, and available macroscopic and histological findings.
- MeSH
- Aortic Aneurysm, Thoracic pathology MeSH
- Aorta pathology MeSH
- Aortic Valve Insufficiency pathology MeSH
- Plaque, Atherosclerotic pathology MeSH
- Adult MeSH
- Ventricular Dysfunction, Left etiology MeSH
- Fibrosis pathology MeSH
- Hyperplasia MeSH
- Humans MeSH
- Macrophages pathology MeSH
- Myocardium pathology MeSH
- Coronary Artery Disease pathology MeSH
- Giant Cell Arteritis pathology MeSH
- Tunica Media pathology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
The field of material surface modification with the aim of biomaterial construction involves several approaches of treatments that allow the preparation of materials, which positively influence adhesion of cells and their proliferation and thus aid and improve tissue formation. Modified materials have a surface composition and morphology intended to interact with biological systems and cellular functions. Not only surface chemistry has an effect on material biological response, surface structures of different morphology can be constructed to guide a desirable biological outcome. Nano-patterned material surfaces have been tested with the aim of how surface geometry and physical properties on a micro- and nano-scale can affect cellular response and influence cell adhesion and proliferation. Biological functionality of solid state substrates was significantly improved by the irradiation of material with plasma discharge or laser treatment. Commonly used "artificial" polymers (e.g. polyethylene (PE), polystyrene (PS), polytetrafluoroethylene (PTFE), polyethylene terephthalate (PET), polyethylene naphthalate (PEN)) and biopolymers (e.g. Poly-l-Lactic acid (PLLA), polymethylpentene (PMP)) were treated with aim of biocompatibility improvement. The treatment of polymer/biopolymer substrates leads to formation of ripple or wrinkle-like structures, supported also with heat treatment or other subsequent surface processing. Several types of chemically different substances (e.g. metal or carbon nano-particles, proteins) were grafted onto material surfaces or built into material structures by different processes. Surface physico-chemical properties (e.g. chemistry, charge, morphology, wettability, electrical conductivity, optical and mechanical properties) of treated surfaces were determined. The enhancement of adhesion and proliferation of cells on modified substrates was investigated in vitro. Bactericidal action of noble metal nano-particles (e.g. Au, Ag) on polymers was characterized. The influence of metal nano-particle grafting by using metal nano-particle suspension prepared by "green" methods was determined. Micro- and nano-patterned surfaces can be constructed as tissue scaffolds with specific functions regarding cell adhesion and proliferation or potential biosensor applications.
... Elastosis Perforans Serpiginosa 276 Striae Distensae 284 -- Reactive Perforating Collagenosis 277 Wrinkling ... ... Chédiak-Higashi Syndrome 442 -- Albinism 440 Reticulate Pigmented Dermatosis of the -- Piebaldism (Patterned ...
6th edit 848 s. : obr., přeruš.bibliogr.
... Shah -- 11.9 Alphabet-pattern strabismus 1221 -- Gory R. Diamond, Raza M. ... ... Gayre -- 12.18 Cosmetic wrinkle reduction with botulinum toxin 1362 -- William J. ...
4th ed. xxiii, 1404 s. : il. ; 29 cm
- MeSH
- Diagnostic Techniques, Ophthalmological MeSH
- Child MeSH
- Adult MeSH
- Eye Diseases MeSH
- Ophthalmologic Surgical Procedures MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Conspectus
- Ortopedie. Chirurgie. Oftalmologie
- NML Fields
- oftalmologie
- NML Publication type
- kolektivní monografie
