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BACKGROUND: Tick carrier proteins are able to bind, transport, and store host-blood heme, and thus they function also as antioxidants. Nevertheless, the role of carrier proteins in ticks is not fully understood. Some of them are found also in tick males which do not feed on hosts to such an extent such as females (there are differences in male feeding in different tick species) and thus they are not dealing with such an excess of heme; some of the carrier proteins were found in salivary glands where the processing of blood and thus release of heme does not occur. Besides, the carrier proteins bind relatively low amounts of heme (in one case only two molecules of heme per protein) compared to their sizes (above 200 kDa). The main aim of this study is the biochemical characterization of a carrier protein from the ornate sheep tick Dermacentor marginatus, hemelipoglycoprotein, with emphasis on its size in native conditions, its glycosylation and identification of its modifying glycans, and examining its carbohydrate-binding specificity. RESULTS: Hemelipoglycoprotein from D. marginatus plasma was purified in native state by immunoprecipitation and denatured using electroelution from SDS-PAGE separated plasma. The protein (290 kDa) contains two subunits with molecular weights 100 and 95 kDa. It is glycosylated by high-mannose and complex N-glycans HexNAc(2)Hex(9), HexNAc(2)Hex(10), HexNAc(4)Hex(7), and HexNAc(4)Hex(8). The purified protein is able to agglutinate red blood cells and has galactose- and mannose-binding specificity. The protein is recognized by antibodies directed against plasma proteins with hemagglutination activity and against fibrinogen-related lectin Dorin M from the tick Ornithodoros moubata. It forms high-molecular weight complexes with putative fibrinogen-related proteins and other unknown proteins under native conditions in tick plasma. Feeding does not increase its amounts in male plasma. The hemelipoglycoprotein was detected also in hemocytes, salivary glands, and gut. In salivary glands, the protein was present in both glycosylated and nonglycosylated forms. CONCLUSION: A 290 kDa hemelipoglycoprotein from the tick Dermacentor marginatus, was characterized. The protein has two subunits with 95 and 100 kDa, and bears high-mannose and complex N-linked glycans. In hemolymph, it is present in complexes with putative fibrinogen-related proteins. This, together with its carbohydrate-binding activity, suggests its possible involvement in tick innate immunity. In fed female salivary glands, it was found also in a form corresponding to the deglycosylated protein.

Článek

Lipoproteínový profil séra pri novozistenej artériovej hypertenzii. Úloha aterogénnych lipoproteínov v patogenéze ochorenia
[Serum lipoprotein profile in newly recognized arterial hypertension. The role of atherogenic lipoproteins in the pathogenesis of disease]

Vnitřní lékařství. 2010 ; 56 (9) : 967-971 příl..

Medvik
Zdroj

Nové vyšetrovacie postupy v biochemickej analýze lipoproteínov umožňujú identifikovať a kvantifikovať aterogénne lipoproteíny plazmy včítane malých denzných LDL a charakterizovať lipoproteínové spektrum ako neaterogénny lipoproteínový profil fenotyp A a aterogénny lipoproteínový profil fenotyp B. Identifikácia neaterogénnej hypercholesterolémie (48 %), aterogénnej hypertriacylglycerolémie (93 %), aterogénnej normolipémie (13 %) u pacientov s artériovou hypertenziou a identifikácia aterogénnej normolipémie u kontrolnej skupiny zdravých probandov (7 %) je zásadným prínosom novej laboratórnej diagnostiky.

New examination approaches in biochemical analysis of lipoproteins can identify and quantify atherogenic plasma lipoproteins, including small dense LDL and characterise a lipoprotein spectrum as a non-atherogenic lipoprotein profile phenotype A, respectively as an atherogenic lipoprotein profile phenotype B. Identification of a non-aterogenic hypercholesterolemia (48%), atherogenic hypertriglyceridemia (93%), atherogenic normolipemia (13%) in patients with arterial hypertension and an atherogenic normolipemia in control group of healthy subjects (7%), is an essential contribution of this new laboratory diagnostics.

Článek

Významné ocenění práce olomoucké lipidologické skupiny

Schneiderka, Petr, 1941-
Autor Autorita

Klinická biochemie a metabolismus. 2010 ; 18-39 (1) : 22.

Medvik
Zdroj

Abstrakt

Role of endoglin in atherogenesis

Atherosklerosa .... 2008 ; () : 62-65.

ISBN 978-80-254-2834-4
Medvik
Zdroj

Článek

The effect of L-arginine on serum lipids and nitrite levels, and number of apoptotic cells, iNOS and eNOS expressions of aorta after formation of fatty streaks in rabbit (Original article)

Journal of Applied Biomedicine. 2008 ; 6 (4) : 203-210.

ISSN 1214-021X
Medvik
Zdroj

L-arginine is the substrate of nitric oxide production which is involved in the regulation of apoptosis and inflammatory processes. The effect of L-arginine after the formation of fatty streaks (FS) is not fully understood in the hypercholesterolemic model and therefore the main objective of this study was to investigate the effect of L-arginine after fatty streaks were developed in the rabbit aorta. Eighteen male rabbits were fed a 1% cholesterol diet for four weeks. One third of the animals were sacrificed randomly to verify fatty streak formation in the aorta (phase I). Then the high cholesterol diet was replaced with a normal diet, and the remaining animals (n=12) were divided into two groups (phase II); group 1 (n=6): normal diet and group 2 (n=6): normal diet plus L-arginine (3% in drinking water). The experiment was continued for a further four weeks. The serum levels of lipids and lipoproteins were increased significantly in phase I (p<0.05), and the fatty streaks score (1±0.44) indicates the formation of lesions in the aorta. In Phase II, no significant differences were observed between the groups for lipids and nitrite concentrations, inducible and endothelial nitric oxide synthase expressions, the number of apoptotic cells, or fatty streak development. These findings show that the administration of 3% L-arginine for four weeks has no beneficial effect in the developed fatty streaks model.

Článek

XXXI. endokrinologické dni s mezinárodní účastí

Vízner, Karel
Autor Autorita

Kazuistiky v diabetologii. 2008 ; 6 (3) : 41-42.

ISSN 1214-231X
Medvik
Zdroj

Abstrakt

Paraoxonasa - patofysiologie a klinické aspekty

Atherosklerosa .... 2007 ; () : S19-S27.

ISBN 978-80-254-0238-2
Medvik
Zdroj

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