We report the design, synthesis, and in vitro evaluation of stimuli-responsive nanoscale micelles that can be activated by light to induce a cytotoxic effect. Micelles were assembled from amphiphilic units made of a photoactivatable ferrocenyl linker, connected on one side to a lipophilic chain, and on the other side to a hydrophilic pegylated chain. In vitro experiments indicated that pristine micelles ("off" state) were nontoxic to MCF-7 cancer cells, even at high concentrations, but became potent upon photoactivation ("on" state). The illumination process led to the dissociation of the micelles and the concomitant release of iron species, triggering cytotoxicity.
- Klíčová slova
- cancer, ferrocene, micelles, nanomedicine, oxidative stress, phototherapy,
- MeSH
- fototerapie MeSH
- metaloceny farmakologie MeSH
- micely * MeSH
- protinádorové látky * MeSH
- železnaté sloučeniny * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ferrocene MeSH Prohlížeč
- metaloceny MeSH
- micely * MeSH
- protinádorové látky * MeSH
- železnaté sloučeniny * MeSH
OBJECTIVES: Due to nano-dimensions (less than 100 nm), can nanoparticles probably penetrate through various membranes and travel from the bloodstream to other organs in the body. The aim of our study was to find out whether NPs Fe3O4 (coated with sodium oleate) injected into the tail vein of laboratory Wistar rats pass through the bloodstream to the respiratory tract (in comparison with a control group); and if so whether increasing doses of NPs Fe3O4 have an escalating harmful effect on selected bronchoalveolar lavage (BAL) parameters. METHODS: Wistar rats were intravenously given 3 doses of the suspension of NPs Fe3O4 (0.1% LD50 = 0.0364, 1.0% = 0.364 and 10.0% = 3.64 mg/kg animal body weight). Seven days later, we sacrificed the animals under anaesthesia, performed bronchoalveolar lavage (BAL), and isolated the collected cells. Many inflammatory and cytotoxic BAL parameters were examined. RESULTS: Both inflammatory and cytotoxic BAL parameters affected by Fe3O4 suspension were changed compared to control results, but not all were statistically significant. Thus, the NPs Fe3O4 passed through the bloodstream to the respiratory tract and affected it. The highest concentration of NPs Fe3O4 (10%) had the most influence on BAL parameters (7 of 12 parameters). Only 3 parameters showed a pure dose dependence. CONCLUSION: We assume that the adverse effect of Fe3O4 NPs in our study is probably not correlated with the dose, but rather with the size of the particles or with their surface area.
- Klíčová slova
- bronchoalveolar lavage, dose dependence, inflammatory and cytotoxic parameters, nanoparticles,
- MeSH
- bronchoalveolární laváž MeSH
- bronchoalveolární lavážní tekutina MeSH
- intravenózní podání MeSH
- krysa rodu Rattus MeSH
- nanočástice * MeSH
- oxid železnato-železitý * MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- oxid železnato-železitý * MeSH
"Core/shell" nanocomposites based on magnetic magnetite (Fe3O4) and redox-active cerium dioxide (CeO2) nanoparticles (NPs) are promising in the field of biomedical interests because they can combine the ability of magnetic NPs to heat up in an alternating magnetic field (AMF) with the pronounced antioxidant activity of CeO2 NPs. Thus, this report is devoted to Fe3O4/CeO2 nanocomposites (NCPs) synthesized by precipitation of the computed amount of "CeO2-shell" on the surface of prefabricated Fe3O4 NPs. The X-ray diffraction, X-ray photoelectron spectroscopy, and high-resolution transmission electron microscopy data validated the formation of Fe3O4/CeO2 "core/shell"-like NCPs, in which ultrafine CeO2 NPs with an average size of approximately 3-3.5 nm neatly surround Fe3O4 NPs. The presence of a CeO2 "shell" significantly increased the stability of Fe3O4/CeO2 NCPs in aqueous suspensions: Fe3O4/CeO2 NCPs with "shell thicknesses" of 5 and 7 nm formed highly stable magnetic fluids with ζ-potential values of >+30 mV. The magnetization values of Fe3O4/CeO2 NCPs decreased with a growing CeO2 "shell" around the magnetic NPs; however, the resulting composites retained the ability to heat efficiently in an AMF. The presence of a CeO2 "shell" generates a possibility to precisely regulate tuning of the maximum heating temperature of magnetic NCPs in the 42-50 °C range and stabilize it after a certain time of exposure to an AMF by changing the thickness of the "CeO2-shell". A great improvement was observed in both antioxidant and antiamyloidogenic activities. It was found that inhibition of insulin amyloid formation, expressed in IC50 concentration, using NCPs with a "shell thickness" of 7 nm was approximately 10 times lower compared to that of pure CeO2. For these NCPs, more than 2 times higher superoxide dismutase-like activity was observed. The coupling of both Fe3O4 and CeO2 results in higher bioactivity than either of them individually, probably due to a synergistic catalytic mechanism.
- Klíčová slova
- antioxidant and antiamyloid activities, cerium dioxide, magnetite, magnetization, morphology, specific loss power, synthesis, “core/shell” nanocomposites,
- MeSH
- antioxidancia * farmakologie MeSH
- magnetické jevy MeSH
- magnetismus MeSH
- nanokompozity * chemie MeSH
- oxid železnato-železitý chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia * MeSH
- oxid železnato-železitý MeSH
DNA three-way junctions (3WJ) represent one of the simplest supramolecular DNA structures arising as intermediates in homologous recombination in the absence of replication. They are also formed transiently during DNA replication. Here we examine the ability of Fe(II)-based metallohelices to act as DNA 3WJ binders and induce DNA damage in cells. We investigated the interaction of eight pairs of enantiomerically pure Fe(II) metallohelices with four different DNA junctions using biophysical and molecular biology methods. The results show that the metallohelices stabilize all types of tested DNA junctions, with the highest selectivity for the Y-shaped 3WJ and minimal selectivity for the 4WJ. The potential of the best stabilizer of DNA junctions and, at the same time, the most selective 3WJ binder investigated in this work to induce DNA damage was determined in human colon cancer HCT116 cells. These metallohelices proved to be efficient in killing cancer cells and triggering DNA damage that could yield therapeutic benefits.
- MeSH
- DNA * chemie MeSH
- konformace nukleové kyseliny MeSH
- lidé MeSH
- nádory * genetika MeSH
- poškození DNA MeSH
- železnaté sloučeniny MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA * MeSH
- železnaté sloučeniny MeSH
Hybrid biological robots (biobots) prepared from living cells are at the forefront of micro-/nanomotor research due to their biocompatibility and versatility toward multiple applications. However, their precise maneuverability is essential for practical applications. Magnetotactic bacteria are hybrid biobots that produce magnetosome magnetite crystals, which are more stable than synthesized magnetite and can orient along the direction of earth's magnetic field. Herein, we used Magnetospirillum magneticum strain AMB-1 (M. magneticum AMB-1) for the effective removal of chlorpyrifos (an organophosphate pesticide) in various aqueous solutions by naturally binding with organic matter. Precision control of M. magneticum AMB-1 was achieved by applying a magnetic field. Under a programed clockwise magnetic field, M. magneticum AMB-1 exhibit swarm behavior and move in a circular direction. Consequently, we foresee that M. magneticum AMB-1 can be applied in various environments to remove and retrieve pollutants by directional control magnetic actuation.
- Klíčová slova
- magnetic actuation, magnetotactic bacteria, micromotors, microrobots, nanorobots,
- MeSH
- Bacteria metabolismus MeSH
- bakteriální proteiny metabolismus MeSH
- dekontaminace MeSH
- Magnetospirillum * metabolismus MeSH
- oxid železnato-železitý * MeSH
- robotika metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- oxid železnato-železitý * MeSH
Selenium can be highly toxic in excess for both animals and humans. However, since its mobile forms can be easily adsorbed with ferric minerals, its mobility in the natural oxic environment is generally not an issue. Still, the removal and immobilization of the long-lived radioactive isotope 79Se from the contaminated anoxic waters is currently a significant concern. 79Se can be accessible in the case of radionuclides' leaching from radioactive waste disposals, where anoxic conditions prevail and where ferrous ions and Fe(II)-bearing minerals predominate after corrosion processes (e.g., magnetite). Therefore, reductive and adsorptive immobilizations by Fe(II)-bearing minerals are the primary mechanisms for removing redox-sensitive selenium. Even though the information on the sorptive interactions of selenium and Fe(II)-bearing minerals seems to be well documented, this review focuses specifically on the state of the available information on the effects of the redox properties of Fe(II)-bearing solid phases (e.g., ferrous oxides, hydroxides, sulfides, and carbonates) on selenium speciation via redox transformation and co-occurring coprecipitation.
- Klíčová slova
- ferrous minerals, nuclear waste, reduction processes, selenium, sorption,
- MeSH
- lidé MeSH
- minerály MeSH
- oxidace-redukce MeSH
- radioaktivní odpad * MeSH
- selen * MeSH
- voda MeSH
- železité sloučeniny MeSH
- železnaté sloučeniny MeSH
- železo MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- minerály MeSH
- radioaktivní odpad * MeSH
- selen * MeSH
- voda MeSH
- železité sloučeniny MeSH
- železnaté sloučeniny MeSH
- železo MeSH
Due to the increasing environmental pollution caused by human activities, environmental remediation has become an important subject for humans and environmental safety. The quest for beneficial pathways to remove organic and inorganic contaminants has been the theme of considerable investigations in the past decade. The easy and quick separation made magnetic solid-phase extraction (MSPE) a popular method for the removal of different pollutants from the environment. Metal-organic frameworks (MOFs) are a class of porous materials best known for their ultrahigh porosity. Moreover, these materials can be easily modified with useful ligands and form various composites with varying characteristics, thus rendering them an ideal candidate as adsorbing agents for MSPE. Herein, research on MSPE, encompassing MOFs as sorbents and Fe3O4 as a magnetic component, is surveyed for environmental applications. Initially, assorted pollutants and their threats to human and environmental safety are introduced with a brief introduction to MOFs and MSPE. Subsequently, the deployment of magnetic MOFs (MMOFs) as sorbents for the removal of various organic and inorganic pollutants from the environment is deliberated, encompassing the outlooks and perspectives of this field.
- MeSH
- adsorpce MeSH
- látky znečišťující životní prostředí * MeSH
- lidé MeSH
- ligandy MeSH
- oxid železnato-železitý MeSH
- porézní koordinační polymery * MeSH
- regenerace a remediace životního prostředí * MeSH
- těžké kovy * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- látky znečišťující životní prostředí * MeSH
- ligandy MeSH
- oxid železnato-železitý MeSH
- porézní koordinační polymery * MeSH
- těžké kovy * MeSH
In this work, the electrochemical properties of the leached sludge, magnetite and zinc ferrite were studied. Acetic acid was used as a leaching reagent because, in recent years, there has been a surge of interest in using zinc-containing materials as photocatalysts, with acetic acid finding application in their preparation. Various methodological approaches were used, but the best results were achieved with a combination of 1-3 h leaching in 0.01 M acetic acid with a solid/liquid ratio of 500. In this arrangement, zincite was almost completely removed from the sludge, while zinc ferrite and magnetite remained in the solid residue. Ex situ analyses of the main leaching products were performed by X-ray diffraction, infrared spectroscopy, and thermogravimetry. The electrochemical behaviour of solid residue and model systems, that are micromagnetite and zinc ferrite, was studied in alkaline media by means of modified carbon paste electrodes, cyclic voltammetry, and chronocoulometry, with a suitable potential window ranging from 0 to 1.5 V. In summary, a linear dependence of the anodic and cathodic peak height on the square root of the scan rate was found. The position of the anodic and cathodic peaks shifted slightly with scan rate, only at low rates, up to 25 mV/s, the individual peaks coincided. The electrochemical response suggested a quasireversible process.
- MeSH
- elektrochemické techniky MeSH
- elektrody MeSH
- ocel * MeSH
- odpadní vody * MeSH
- oxid železnato-železitý MeSH
- uhlík chemie MeSH
- železité sloučeniny MeSH
- zinek chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ferrite MeSH Prohlížeč
- ocel * MeSH
- odpadní vody * MeSH
- oxid železnato-železitý MeSH
- uhlík MeSH
- železité sloučeniny MeSH
- zinek MeSH
Non-psychotropic cannabinoids (e.g., cannabidiol, cannabinol and cannabigerol) are contained in numerous alimentary and medicinal products. Therefore, predicting and studying their possible side effects, such as changes in DNA methylation, is an important task for assessing the safety of these products. Interference with TET enzymes by chelating ferrous ions can contribute to the altered methylation pattern. All tested cannabinoids displayed a strong affinity for Fe(II) ions. Cannabidiol and cannabinol exhibited potent inhibitory activities (IC50 = 4.8 and 6.27 μM, respectively) towards the TET1 protein, whereas cannabigerol had no effect on the enzyme activity. An in silico molecular docking study revealed marked binding potential within the catalytic cavity for CBD/CBN, but some affinity was also found for CBG, thus the total lack of activity remains unexplained. These results imply that cannabinoids could affect the activity of the TET1 protein not only due to their affinity for Fe(II) but also due to other types of interactions (e.g., hydrophobic interactions and hydrogen bonding).
- Klíčová slova
- Cannabinoids, DNA methylation, Epigenetic, Iron chelation, TET1 protein,
- MeSH
- Cannabis * chemie MeSH
- kanabidiol * chemie farmakologie MeSH
- kanabinoidy * farmakologie MeSH
- kanabinol farmakologie MeSH
- simulace molekulového dockingu MeSH
- železnaté sloučeniny MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kanabidiol * MeSH
- kanabinoidy * MeSH
- kanabinol MeSH
- železnaté sloučeniny MeSH
The Madagascar's periwinkle is the model plant for studies of plant specialized metabolism and monoterpenoid indole alkaloids (MIAs), and an important source for the anticancer medicine vinblastine. The elucidation of entire 28-step biosynthesis of vinblastine allowed further investigations for the formation of other remarkably complex bioactive MIAs. In this study, we describe the discovery and characterization of vindolinine synthase, a Fe(II)/α-ketoglutarate-dependent (Fe/2OG) dioxygenase, that diverts assembly of tabersonine to vinblastine toward the formation of three alternatively cyclized MIAs: 19S-vindolinine, 19R-vindolinine, and venalstonine. Vindolinine synthase catalyzes a highly unusual, redox-neutral reaction to form a radical from dehydrosecodine, which is further cyclized by hydrolase 2 to form the three MIA isomers. We further show the biosynthesis of vindolinine epimers from tabersonine using hydrolase 2 catalyzed reverse cycloaddition. While the occurrence of vindolinines is rare in nature, the more widely found venalstonine derivatives are likely formed from similar redox-neutral reactions by homologous Fe/2OG dioxygenases.
- MeSH
- Catharanthus * metabolismus MeSH
- gen pro FTO metabolismus MeSH
- hydrolasy metabolismus MeSH
- oxidace-redukce MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné proteiny genetika MeSH
- sekologanin-tryptaminové alkaloidy * metabolismus MeSH
- vinblastin analogy a deriváty metabolismus MeSH
- železnaté sloučeniny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- gen pro FTO MeSH
- hydrolasy MeSH
- rostlinné proteiny MeSH
- sekologanin-tryptaminové alkaloidy * MeSH
- vinblastin MeSH
- vindolinine MeSH Prohlížeč
- železnaté sloučeniny MeSH
