Background: Interleukin-6 (IL-6) is a pleiotropic cytokine with a multitude of pro-inflammatory effects. Serum C-reactive protein (CRP) is an acute phase protein induced mainly by IL-6 in response to inflammatory conditions, particularly infection. The biological functions of CRP include opsonisation, induction of phagocytosis, complement activation, or chemotaxis enhancement. Factors interfering with IL-6-mediated recruitment of innate immune responses, such as the presence of anti-IL6 antibodies, may therefore compromise the host resistance to microbial pathogens. This has major implications for the use of IL-6-targeting biologics, such as tocilizumab or sarilumab in rheumatologic, immune dysregulation diseases, and cancer. Case presentation: 20-month-old Czech female developed severe septic shock with clinical and laboratory signs of systemic inflammation but no increase of CRP or IL-6. The offending pathogen was most likely Staphylococcus aureus, detected in a throat swab; the response to antibiotic treatment was prompt. A defect in the integrity of IL-6/CRP axis was suspected and verified by the detection of neutralizing IL-6 antibodies in the serum of the child. Conclusion: We report a first case of systemic bacterial infection in a patient with anti-IL6 autoantibodies. Disturbed IL-6 signaling, whether iatrogenic by targeted IL-6 blockade or endogenous due to the presence of autoantibodies against IL-6, represents a risk factor for increased infectious susceptibility. Patients with severe bacterial infection without elevation of CRP should be examined for the presence of anti-IL6 autoantibodies.

• Klíčová slova
• C-reactive protein, anti-IL6 autoantibodies, interleukin 6, sarilumab, siltuximab, tocilizumab,
• MeSH
• autoprotilátky krev   MeSH
• C-reaktivní protein MeSH
• interleukin-6 imunologie   MeSH
• kojenec MeSH
• lidé MeSH
• septický šok krev   imunologie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• autoprotilátky MeSH
• C-reaktivní protein MeSH
• interleukin-6 MeSH

• MeSH
• autoprotilátky analýza   MeSH
• Klinefelterův syndrom imunologie   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• autoprotilátky MeSH

The presence of ZP autoantibodies in serum and Ffl samples of 109 women attending the ART Center in Kiev was investigated using IIF and ELISA. Positive serum and Ffl samples examined by both methods were found in 20 (18.34%) and in 19 (17.43%) patients respectively; 31 (28.44%) serum samples and 33 (30.27 %) Ffl samples analyzed by IIF were positive; of the samples analyzed by ELISA 21 (19.26%) and 20 (18.34%), respectively, were positive. No relationship was found between ZP autoantibody incidence and the type and cause of infertility. A significant prevalence of ZP autoantibodies detected by ELISA in Ffl was found in patients with fertilization failure (39.13%) and with low fertilization rate (42.85%) when compared to patients with middle fertilization rate (5.71%) and high fertilization rate (8.1%). The clinical significance of ZP autoantibodies in Ffls for in vitro fertilization outcome was suggested.

• MeSH
• autoprotilátky krev   imunologie   MeSH
• dospělí MeSH
• ELISA MeSH
• fertilizace in vitro * MeSH
• fertilizace imunologie   MeSH
• lidé MeSH
• ovariální folikul imunologie   MeSH
• tělesné tekutiny imunologie   MeSH
• ženská infertilita etiologie   imunologie   terapie   MeSH
• zona pellucida imunologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• autoprotilátky MeSH

Autoantibodies directed against various self-antigens comprise a heterogeneous group of immunoglobulins, which differ in their qualitative and quantitative features. An important qualitative characteristic of antibodies is affinity/avidity, which changes in the process of its maturation during the immune response.This study is aimed to summarize the knowledge about avidity of selected autoantibodies in certain autoimmune diseases. The avidity of various autoantibodies differs under distinct clinical situations. High-, moderate or low-avidity may be found in biological fluids in patients with autoimmune diseases.The avidity maturation associated with progression from low to high values typical for antibodies against exogenous antigens is not always uniform in autoimmune diseases; therefore, the avidity of each autoantibody should be judged individually. Some studies promise the possible benefit of avidity examination for the refinement of diagnosis and prediction of selected autoimmune diseases.Key words: affinity - anti-citrullinated protein antibodies - anti-glomerular basement membrane antibodies - anti-glutamate decarboxylase antibodies - anti-insulin antibodies - autoantibodies - avidity - onconeuronal antibodies.

• MeSH
• afinita protilátek MeSH
• autoantigeny * MeSH
• autoimunitní nemoci diagnóza   imunologie   MeSH
• autoprotilátky * MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiglomerular basement membrane antibody MeSH Prohlížeč
• autoantigeny * MeSH
• autoprotilátky * MeSH

• Klíčová slova
• AUTOANTIBODIES *, HYPOPITUITARISM *, PREGNANCY *, SHEEHAN'S SYNDROME *,
• MeSH
• autoprotilátky * MeSH
• hypopituitarismus * MeSH
• lidé MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• autoprotilátky * MeSH

Three serum autoantibodies against antigens of the thyroid gland were detected by the indirect immunofluorescence in a Beagle dog breeding colony. The anti-thyroglobulin autoantibody was present in 10.0%, the anti-microsomal antigen autoantibody was present in 24.6%, and the anti-nuclear substance autoantibody in 10.8% of examined sera, respectively. Only the presence of anti-thyroglobulin autoantibodies could be correlated with histologic findings of the lymphocytic thyroiditis in the same colony.

• MeSH
• autoimunitní tyreoiditida genetika   imunologie   veterinární   MeSH
• autoprotilátky analýza   MeSH
• nemoci psů genetika   imunologie   MeSH
• psi MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• autoprotilátky MeSH

Data concerning autoantibodies in hypertrophic cardiomyopathy are rare and controversial and only the results of indirect immunofluorescence methods are available in the literature. The aim of this study was to determine the frequency and clinical significance of autoantibodies in hypertrophic cardiomyopathy by means of the Western blot which is a highly sensitive immunological technique. Sera from twelve (48.0%) of the whole group of 25 patients with hypertrophic cardiomyopathy reacted with actin from the HeLa extract. The incidence of anti-actin antibodies in sera from 23 ischaemic heart disease patients was 13.0% (P less than 0.025) and in 37 apparently healthy subjects was 8.1% (P less than 0.001). When samples were assayed on the actomyosin prepared from Xenopus laevis muscle, sera of 14 (56.0%) of the patients exhibited actin positivity while the positivity in ischaemic heart disease and in healthy subjects was found to be 13.0 and 13.5%, respectively (P less than 0.001). Presence of anti-actin antibodies was related to the functional and clinical status and the progression of the disease. Anti-actin antibodies were found more frequently in medically treated patients. These data suggest that anti-actin antibodies are probably not directly involved in the pathogenesis of hypertrophic cardiomyopathy, but their detection might be useful as an additional marker of disease severity.

• MeSH
• aktiny imunologie   MeSH
• autoprotilátky analýza   MeSH
• dospělí MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• ELISA MeSH
• HeLa buňky MeSH
• hypertrofická kardiomyopatie diagnóza   imunologie   MeSH
• lidé MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• aktiny MeSH
• autoprotilátky MeSH

Results of experimental studies on mice concerning the important role of cells producing normal autoantibodies (AAPC) in the reaction of the organism to specific antigens have been systematized. The number of AAPC in the blood and organs increases as early as 15-20 min after the administration of antigen and this increase is particularly intensive during reimmunization. On the other hand, the administration of heterologous antigens to the same mice causes a decrease in the number of AAPC. Transfer of blood plasma from immunized donors induces an increase in the number of AAPC in the recipients. The addition of specific antigen to blood preparations conditions a considerable increase in the number and size of AAPC in immunized humans and animals. It has been demonstrated that one and the same cell produces both auto-antibodies and specific hemolysins. A hypothesis has been proposed dealing with the stages of involvement of autoantibody-producing cells in the immune response and with a new type of intercellular interactions between erythrocytes and karyocytes.

• MeSH
• autoantigeny imunologie   MeSH
• autoprotilátky imunologie   MeSH
• B-lymfocyty imunologie   MeSH
• buňky produkující protilátky imunologie   MeSH
• hemolytická plaková technika MeSH
• hemolýza MeSH
• imunizace MeSH
• imunoglobuliny MeSH
• lidé MeSH
• mezibuněčná komunikace MeSH
• myši MeSH
• T-lymfocyty imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• autoantigeny MeSH
• autoprotilátky MeSH
• imunoglobuliny MeSH

Autoantibodies against galactose-deficient IgA1 drive formation of pathogenic immune complexes in IgA nephropathy. IgG autoantibodies against galactose-deficient IgA1 in patients with IgA nephropathy have a specific amino-acid sequence, Y1CS3, in the complementarity-determining region 3 of the heavy chain variable region compared with a Y1CA3 sequence in similar isotype-matched IgG from healthy controls. We previously found that the S3 residue is critical for binding galactose-deficient IgA1. To determine whether this difference is due to a rare germline sequence, we amplified and sequenced the corresponding germline variable region genes from peripheral blood mononuclear cells of seven patients with IgA nephropathy and six healthy controls from whom we had cloned single-cell lines secreting monoclonal IgG specific for galactose-deficient IgA1. Sanger DNA sequencing revealed that complementarity-determining region 3 in the variable region of the germline genes encoded the Y1C(A/V)3 amino-acid sequence. Thus, the A/V>S substitution in the complementarity-determining region 3 of anti-galactose-deficient-IgA1 autoantibodies of the patients with IgA nephropathy is not a rare germline gene variant. Modeling analyses indicated that the S3 hydroxyl group spans the complementarity-determining region 3 loop stem, stabilizing the adjacent β-sheet and stem structure, important features for effective binding to galactose-deficient IgA1. Understanding processes leading to production of the autoantibodies may offer new approaches to treat IgA nephropathy.

• Klíčová slova
• IgA nephropathy, immune complexes, immunology,
• MeSH
• autoprotilátky genetika   MeSH
• galaktosa nedostatek   MeSH
• IgA nefropatie enzymologie   genetika   imunologie   MeSH
• imunoglobulin A * MeSH
• lidé MeSH
• mutace * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• autoprotilátky MeSH
• galaktosa MeSH
• imunoglobulin A * MeSH

In an investigation of autoimmune antibodies using indirect immunofluorescence and Western blot analysis in a group of porphyria cutanea tarda patients we did not find any cytosolic antibodies potentially able to inhibit uroporphyrinogen decarboxylase. Furthermore, no known etiological factors were present in any of our patients. We therefore consider the development of the recently reported autoantibody with a molecular weight of 40 kDa a reaction to infection with the hepatitis C virus. The origin of mostly antinuclear antibodies against liver antigens (50, 45 and 56 kDa), detected in seven patients, was not identified and their etiopathogenetic implications remain unknown.

• MeSH
• antigeny imunologie   MeSH
• antinukleární protilátky analýza   imunologie   MeSH
• autoprotilátky analýza   fyziologie   MeSH
• cytosol imunologie   MeSH
• fluorescenční protilátková technika nepřímá MeSH
• hepatitida C - protilátky analýza   MeSH
• imunoblotting MeSH
• játra imunologie   MeSH
• koproporfyriny moč   MeSH
• lidé MeSH
• osmolární koncentrace MeSH
• porfyriny moč   MeSH
• porphyria cutanea tarda imunologie   moč   MeSH
• uroporfyrinogendekarboxylasa antagonisté a inhibitory   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny MeSH
• antinukleární protilátky MeSH
• autoprotilátky MeSH
• hepatitida C - protilátky MeSH
• koproporfyriny MeSH
• porfyriny MeSH
• uroporfyrinogendekarboxylasa MeSH