INTRODUCTION: The Raise Awareness of Influenza Strategies in Europe (RAISE) group gathered information about the healthcare burden of influenza (hospitalizations, intensive care unit [ICU] admissions, and excess deaths), surveillance systems, and the vaccine coverage rate (VCR) in older adults in 18 European countries and Israel. AREAS COVERED: Published medical literature and official medical documentation on the influenza disease burden in the participating countries were reviewed from 2010/11 until the 2022/23 influenza seasons. Information on the framework for monitoring the disease burden and the provision for ensuring older adults had access to vaccination in their respective countries was provided. Data on influenza VCR in older adults were collected for the 2019/20 to 2022/23 influenza seasons. Data are reported descriptively. EXPERT OPINION: Influenza presents a significant healthcare burden in older adults. Reporting outcomes across participating countries is heterogeneous, highlighting the need for standardized approaches. Although older adults receive free influenza vaccination, vaccine uptake is highly variable among countries. Moreover, hospitalization rates remain high even in countries reporting a high VCR. Increased awareness and education on the burden of disease and the broader use of improved influenza vaccines for older adults may help reduce the disease burden on this population.

• Keywords
• Influenza, Influenza surveillance, burden of disease, older adults, vaccine coverage rate,
• MeSH
• Influenza, Human * prevention & control   epidemiology   MeSH
• COVID-19 * prevention & control   epidemiology   MeSH
• Hospitalization * statistics & numerical data   MeSH
• Humans MeSH
• Vaccination Coverage * statistics & numerical data   MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Influenza Vaccines * administration & dosage   MeSH
• Check Tag
• Humans MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Publication type
• Journal Article MeSH
• Review MeSH
• Geographicals
• Europe epidemiology   MeSH
• Israel epidemiology   MeSH
• Names of Substances
• Influenza Vaccines * MeSH

Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives.

• Keywords
• TRY plant trait database, data coverage, data integration, data representativeness, functional diversity, plant traits,
• MeSH
• Biodiversity MeSH
• Ecology MeSH
• Ecosystem * MeSH
• Access to Information * MeSH
• Plants MeSH
• Publication type
• Journal Article MeSH

OBJECTIVES: Individuals with HIV infection are at an increased risk for a number of infectious diseases, some of which are preventable by vaccination. Unfortunately, little is known about the attitudes of this population group to vaccination, therefore, we decided to find out vaccination coverage against 5 infections among newly diagnosed HIV-infected patients in the Czech Republic. METHODS: This cross-sectional study was conducted on newly diagnosed patients who started their follow-up care at the HIV Clinic of Na Bulovce Hospital during the two following years. Vaccination history data and results of serological tests were collected from all participants. RESULTS: Enrolled were 269 HIV-positive subjects (94.1% males) with a mean age of 34.4 years, 64 subjects (23.8%) had tertiary education, 229 (85.1%) were men having sex with men, 32 (11.9%) were heterosexual, and 8 (3.0%) were injection drug users. The mean CD4+ T-lymphocyte count was 556.2/µL, with 149 persons (55.4%) who had a CD4+ T-lymphocyte count > 500/µL, and 68 (25.3%) individuals were late presenters with CD4+ T-lymphocyte count < 350/µL. A vaccination against tetanus was reported by 262 subjects (97.4%), against influenza by 18 subjects (6.7%), against tick-borne encephalitis by 18 subjects (6.7%), against viral hepatitis A by 78 persons (29.0%), and against hepatitis B by 104 subjects (38.7%). For influenza, tick-borne encephalitis and hepatitis A, a significant positive impact of tertiary education was found (p-values < 0.001-0.044). Vaccination coverage against both types of hepatitis was significantly lower in late presenters (p = 0.044 and p = 0.004, respectively). CONCLUSIONS: Vaccination rates found in our cohort were except tetanus and hepatitis B in young people low, especially for influenza and tick-borne encephalitis. Higher level of education and less advanced HIV infection were associated with higher vaccination rates. To improve this unsatisfactory situation, more attention should be paid to vaccination.

• Keywords
• HIV, hepatitis, influenza, tetanus, tick-borne encephalitis, vaccination coverage,
• MeSH
• Adult MeSH
• HIV Infections * diagnosis   MeSH
• Humans MeSH
• Adolescent MeSH
• CD4 Lymphocyte Count methods   MeSH
• Vaccination Coverage * MeSH
• Cross-Sectional Studies MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Czech Republic MeSH

Seasonal influenza can have serious morbid consequences and can even result in death, particularly in at-risk populations, including healthcare professionals (HCPs), elderly and those living with a medical risk condition. Although in Europe recommendations exist for annual influenza vaccination in these populations in most countries, the vaccination coverage rate (VCR) is often well below the World Health Organization target of 75% coverage. In our previous survey in 2009 we showed that some elements of national vaccination policies, e.g. reminder systems, strong official recommendation, and easy access, seemed to contribute to achieving higher influenza VCRs among elderly. We repeated the survey in 2016, using the same methodology to assess changes in influenza VCRs among the elderly and in the impact of policy elements on these VCRs. In addition, we collected information about VCRs among HCPs, and those living with a medical risk condition. The median VCR in the 21 countries that had recommendations for influenza vaccination in the elderly was 35.3%, ranging from 1.1% in Estonia to 74.5% in Scotland. The average VCRs for HCPs and those living with medical risk conditions, available in 17 and 10 countries, respectively, were 28.3% (range 7% in Czech Republic to 59.1% in Portugal) and 32.2% (range from 20.0% in the Czech Republic and Hungary to 59.6% in Portugal), respectively. Fewer countries were able to provide data from HCP and those living with medical risk conditions. Since the initial survey during the 2007-2008 influenza season, VCRs have decreased in the elderly in the majority of countries, thus, achieving high VCRs in the elderly and the other target groups is still a major public health challenge in Europe. This could be addressed by the identification, assessment and sharing of best practice for influenza vaccination policies.

• Keywords
• Elderly, Seasonal influenza vaccination, Vaccination coverage, Vaccination policies, healthcare professionals, influenza, policy,
• MeSH
• Influenza, Human prevention & control   MeSH
• Infant MeSH
• Middle Aged MeSH
• Humans MeSH
• Immunization Programs organization & administration   statistics & numerical data   MeSH
• Vaccination Coverage statistics & numerical data   MeSH
• Child, Preschool MeSH
• Seasons MeSH
• Aged MeSH
• Pregnancy MeSH
• Health Policy * MeSH
• Health Plan Implementation organization & administration   statistics & numerical data   MeSH
• Check Tag
• Infant MeSH
• Middle Aged MeSH
• Humans MeSH
• Child, Preschool MeSH
• Aged MeSH
• Pregnancy MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Europe MeSH

Due to the technical advances of mass spectrometers, particularly increased scanning speed and higher MS/MS resolution, the use of data-independent acquisition mass spectrometry (DIA-MS) became more popular, which enables high reproducibility in both proteomic identification and quantification. The current DIA-MS methods normally cover a wide mass range, with the aim to target and identify as many peptides and proteins as possible and therefore frequently generate MS/MS spectra of high complexity. In this report, we assessed the performance and benefits of using small windows with, e.g., 5-m/z width across the peptide elution time. We further devised a new DIA method named RTwinDIA that schedules the small isolation windows in different retention time blocks, taking advantage of the fact that larger peptides are normally eluting later in reversed phase chromatography. We assessed the direct proteomic identification by using shotgun database searching tools such as MaxQuant and pFind, and also Spectronaut with an external comprehensive spectral library of human proteins. We conclude that algorithms like pFind have potential in directly analyzing DIA data acquired with small windows, and that the instrumental time and DIA cycle time, if prioritized to be spent on small windows rather than on covering a broad mass range by large windows, will improve the direct proteome coverage for new biological samples and increase the quantitative precision. These results further provide perspectives for the future convergence between DDA and DIA on faster MS analyzers.

• Keywords
• Data-independent acquisition, Isolation windows, Maxquant, Spectronaut, pFind,
• MeSH
• Chromatography, Reverse-Phase MeSH
• Mass Spectrometry methods   MeSH
• Humans MeSH
• Cell Line, Tumor MeSH
• Peptides analysis   MeSH
• Proteins analysis   MeSH
• Proteomics methods   MeSH
• Software MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Peptides MeSH
• Proteins MeSH

AIM: To map the current seroprevalence of antibodies and vaccine coverage against viral hepatitis A (VHA) in the general population of the South Moravian Region (SMR). MATERIAL AND METHODS: During the VHA outbreak in 2016, nearly 3500 persons were investigated epidemiologically by the Regional Public Health Authority of the SMR within the anti-epidemic measures. The data collected were, among others, basic personal data and information whether the person has been vaccinated against VHA. For the reportedly vaccinated, the information was checked in the GP records or vaccination card. Those who reported to be unvaccinated were referred for serological testing. For the purposes of this retrospective prevalence study, available data on 3130 persons were analysed. The study population was divided into 10 age groups (0-4 years, 5-9 years, 10-14 years, 15-19 years, 20-24 years, 25-34 years, 35-44 years, 45-54 years, 55-65 years, and 65 years and over). The numbers of vaccinated persons with positive total antibodies and of those who tested negative were determined. The data obtained were statistically analysed using the Microsoft Excel tabular processor. RESULTS: Of the study cohort of 3130 persons, 659 (21.05 %) turned out to be seropositive, and 320 (10.22 %) of the seropositives were vaccinated. The seroprevalence rates in the age groups over 35 years were increasing with increasing age, being the highest (51.40 %) in those aged 65 years and older. The highest vaccine coverage rates were achieved in the age groups 10-14 years (20.73 %) and 15-19 years (20.77 %). CONCLUSION: The seroprevalence rates of antibodies against VHA in the general population of the SMR are very low (21.05 %), being even lower than previously reported. The vaccine coverage rate against VHA is 10.22 %. The results show higher vaccine coverage rates in those born after the vaccine against VHA was implemented. Children show the highest vaccine coverage rates. Adults tend to get vaccinated less often. This fact promotes the risk of infection spread and outbreaks, as recently evidenced by the spread of infection from persons engaging in risky behaviours to the general population of young working-age adults.

• Keywords
• viral hepatitis A - seroprevalence - vaccine coverage rate - susceptibility.,
• MeSH
• Child MeSH
• Adult MeSH
• Hepatitis A * epidemiology   MeSH
• Humans MeSH
• Retrospective Studies MeSH
• Aged MeSH
• Seroepidemiologic Studies MeSH
• Vaccination statistics & numerical data   MeSH
• Vaccines * MeSH
• Age Factors MeSH
• Check Tag
• Child MeSH
• Adult MeSH
• Humans MeSH
• Aged MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Czech Republic epidemiology   MeSH
• Names of Substances
• Vaccines * MeSH

Incomplete stent apposition and uncovered struts are associated with a higher risk of stent thrombosis. No data exist on the process of neointimal coverage and late apposition status of the bioresorbable vascular scaffold (BVS) when implanted in the highly thrombogenic setting of ST-segment elevation acute myocardial infarction (STEMI). The aim of this study was to assess the serial changes in strut apposition and early neointimal coverage of the BVS using optical coherence tomography (OCT) in selected patients enrolled in the PRAGUE-19 study. Intracoronary OCT was performed in 50 patients at the end of primary percutaneous coronary intervention for acute STEMI. Repeated OCT of the implanted BVS was performed in 10 patients. Scaffold area, scaffold mean diameter and incomplete strut apposition (ISA) were compared between baseline and control OCT. Furthermore, strut neointimal coverage was assessed during the control OCT. Mean scaffold area and diameter did not change between the baseline and control OCT (8.59 vs. 9.06 mm(2); p = 0.129 and 3.31 vs. 3.37 mm; p = 0.202, respectively). Differences were observed in ISA between the baseline and control OCT (0.63 vs. 1.47 %; p < 0.05). We observed 83.1 % covered struts in eight patients in whom the control OCT was performed 4-6 weeks after BVS implantation, and 100 % covered struts in two patients 6 months after BVS implantation. Persistent strut apposition and early neointimal coverage were observed after biodegradable vascular scaffold implantation in patients with acute ST-segment elevation myocardial infarction.

• Keywords
• Acute myocardial infarction, Biodegradable vascular scaffold, Late apposition, Neointimal coverage,
• MeSH
• Coated Materials, Biocompatible * MeSH
• Time Factors MeSH
• Everolimus administration & dosage   adverse effects   MeSH
• ST Elevation Myocardial Infarction diagnostic imaging   therapy   MeSH
• Cardiovascular Agents administration & dosage   adverse effects   MeSH
• Coronary Angiography MeSH
• Percutaneous Coronary Intervention adverse effects   instrumentation   MeSH
• Coronary Vessels diagnostic imaging   drug effects   MeSH
• Middle Aged MeSH
• Humans MeSH
• Neointima * MeSH
• Tomography, Optical Coherence MeSH
• Prospective Studies MeSH
• Prosthesis Design MeSH
• Aged MeSH
• Absorbable Implants * MeSH
• Treatment Outcome MeSH
• Check Tag
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Clinical Study MeSH
• Geographicals
• Czech Republic MeSH
• Names of Substances
• Coated Materials, Biocompatible * MeSH
• Everolimus MeSH
• Cardiovascular Agents MeSH

In the evolving landscape of Industry 4.0, the integration of advanced wireless technologies into manufacturing processes holds the promise of unprecedented connectivity and efficiency. In particular, the data transmission in a heavy industry environment needs stable connectivity with mobile operators. This paper deals with the performance study of 4G and 5G mobile signal coverage within a complex factory environment. For this purpose, a cost-effective and portable measurement setup was realized and used to provide long-term measurement campaigns monitoring and recording several key parameter indicators (KPIs) in 4G/5G downlink and upload. To support the reproducibility of the provided study and other research activities, the measured dataset is publicly available for download. Among others findings, the obtained results show how the performance of 4G/5G is influenced by a heavy industry environment and of the time of day on the network load.

• Keywords
• 4G, 5G, coverage mapping, industrial factory, key performance indicators, mobile networks,
• Publication type
• Journal Article MeSH

BACKGROUND: The Meningococcal Antigen Typing System (MATS) was developed to identify meningococcus group B strains with a high likelihood of being covered by the 4CMenB vaccine, but is limited by the requirement for viable isolates from culture-confirmed cases. We examined if antigen genotyping could complement MATS in predicting strain coverage by the 4CMenB vaccine. METHODS: From a panel of 3912 MATS-typed invasive meningococcal disease isolates collected in England and Wales in 2007-2008, 2014-2015 and 2015-2016, and in 16 other countries in 2000-2015, 3481 isolates were also characterized by antigen genotyping. Individual associations between antigen genotypes and MATS coverage for each 4CMenB component were used to define a genetic MATS (gMATS). gMATS estimates were compared with England and Wales human complement serum bactericidal assay (hSBA) data and vaccine effectiveness (VE) data from England. RESULTS: Overall, 81% of the strain panel had genetically predictable MATS coverage, with 92% accuracy and highly concordant results across national panels (Lin's accuracy coefficient, 0.98; root-mean-square deviation, 6%). England and Wales strain coverage estimates were 72-73% by genotyping (66-73% by MATS), underestimating hSBA values after four vaccine doses (88%) and VE after two doses (83%). The gMATS predicted strain coverage in other countries was 58-88%. CONCLUSIONS: gMATS can replace MATS in predicting 4CMenB strain coverage in four out of five cases, without requiring a cultivable isolate, and is open to further improvement. Both methods underestimated VE in England. Strain coverage predictions in other countries matched or exceeded England and Wales estimates.

• Keywords
• 4CMenB vaccine, Genotyping, Neisseria meningitidis serogroup B, Strain coverage, gMATS,
• MeSH
• Antigens, Bacterial genetics   MeSH
• Global Health MeSH
• Genotype * MeSH
• Genotyping Techniques methods   MeSH
• Humans MeSH
• Meningitis, Meningococcal epidemiology   microbiology   MeSH
• Meningococcal Vaccines immunology   MeSH
• Molecular Epidemiology methods   MeSH
• Neisseria meningitidis, Serogroup B classification   genetics   isolation & purification   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 4CMenB vaccine MeSH Browser
• Antigens, Bacterial MeSH
• Meningococcal Vaccines MeSH

CATH (https://www.cathdb.info) identifies domains in protein structures from wwPDB and classifies these into evolutionary superfamilies, thereby providing structural and functional annotations. There are two levels: CATH-B, a daily snapshot of the latest domain structures and superfamily assignments, and CATH+, with additional derived data, such as predicted sequence domains, and functionally coherent sequence subsets (Functional Families or FunFams). The latest CATH+ release, version 4.3, significantly increases coverage of structural and sequence data, with an addition of 65,351 fully-classified domains structures (+15%), providing 500 238 structural domains, and 151 million predicted sequence domains (+59%) assigned to 5481 superfamilies. The FunFam generation pipeline has been re-engineered to cope with the increased influx of data. Three times more sequences are captured in FunFams, with a concomitant increase in functional purity, information content and structural coverage. FunFam expansion increases the structural annotations provided for experimental GO terms (+59%). We also present CATH-FunVar web-pages displaying variations in protein sequences and their proximity to known or predicted functional sites. We present two case studies (1) putative cancer drivers and (2) SARS-CoV-2 proteins. Finally, we have improved links to and from CATH including SCOP, InterPro, Aquaria and 2DProt.

• MeSH
• Molecular Sequence Annotation MeSH
• COVID-19 epidemiology   prevention & control   virology   MeSH
• Databases, Protein statistics & numerical data   MeSH
• Epidemics MeSH
• Internet MeSH
• Humans MeSH
• Protein Domains * MeSH
• Proteins chemistry   genetics   metabolism   MeSH
• SARS-CoV-2 genetics   metabolism   physiology   MeSH
• Amino Acid Sequence MeSH
• Sequence Analysis, Protein methods   MeSH
• Sequence Homology, Amino Acid MeSH
• Viral Proteins chemistry   genetics   metabolism   MeSH
• Computational Biology methods   statistics & numerical data   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Proteins MeSH
• Viral Proteins MeSH