Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
55005623
Howard Hughes Medical Institute - United States
PubMed
19302539
DOI
10.1111/j.1462-2920.2009.01911.x
PII: EMI1911
Knihovny.cz E-resources
- MeSH
- Staining and Labeling methods MeSH
- Time Factors MeSH
- Microscopy, Confocal methods MeSH
- Membrane Transport Proteins biosynthesis MeSH
- Gene Expression Regulation, Fungal * MeSH
- Recombinant Fusion Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae Proteins biosynthesis MeSH
- Saccharomyces cerevisiae enzymology growth & development MeSH
- Green Fluorescent Proteins genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ATO1 protein, S cerevisiae MeSH Browser
- Membrane Transport Proteins MeSH
- Recombinant Fusion Proteins MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Green Fluorescent Proteins MeSH
Yeasts, when growing on solid surfaces, form organized multicellular structures, colonies, in which cells differentiate and thus possess different functions and undergo dissimilar fate. Understanding the principles involved in the formation of these structures requires new approaches that allow the study of individual cells directly in situ without needing to remove them from the microbial community. Here we introduced a new approach to the analysis of whole yeast microcolonies either containing specific proteins labelled by fluorescent proteins or stained with specific dyes, by two-photon excitation confocal microscopy. It revealed that the colonies are covered with a thin protective skin-like surface cell layer which blocks penetration of harmful compounds. The cells forming the layer are tightly connected via cell walls, the presence of which is essential for keeping of protective layer function. Viewing the colonies from different angles allowed us to reconstruct a three-dimensional profile of the cells producing ammonium exporter Ato1p within developing microcolonies growing either as individuals or within a group of microcolonies. We show that neighbouring microcolonies coordinate production of Ato1p-GFP. Ato1p itself appears synchronously in cells, which do not originate from the same ancestor, but occupy specific position within the colony.
References provided by Crossref.org
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