Impairment of the autoregulation of renal hemodynamics and of the pressure-natriuresis relationship precedes the development of hypertension in Cyp1a1-Ren-2 transgenic rats
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Aldosterone blood MeSH
- Angiotensin II metabolism MeSH
- Angiotensin II Type 1 Receptor Blockers pharmacology MeSH
- Time Factors MeSH
- Cytochrome P-450 CYP1A1 genetics MeSH
- Hemodynamics drug effects MeSH
- Glomerular Filtration Rate drug effects MeSH
- Homeostasis * MeSH
- Hypertension genetics physiopathology MeSH
- Indoles administration & dosage MeSH
- Blood Pressure drug effects MeSH
- Rats MeSH
- Kidney blood supply drug effects MeSH
- Losartan pharmacology MeSH
- Natriuresis * MeSH
- Rats, Transgenic MeSH
- Receptor, Angiotensin, Type 1 metabolism MeSH
- Renal Circulation drug effects MeSH
- Renin-Angiotensin System physiology MeSH
- Renin genetics MeSH
- Sodium metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aldosterone MeSH
- Angiotensin II MeSH
- Angiotensin II Type 1 Receptor Blockers MeSH
- Cytochrome P-450 CYP1A1 MeSH
- indole-3-carbinol MeSH Browser
- Indoles MeSH
- Losartan MeSH
- Receptor, Angiotensin, Type 1 MeSH
- Renin MeSH
- Sodium MeSH
OBJECTIVE: The present study was performed to characterize the autoregulatory efficiency of renal blood flow and glomerular filtration rate and the pressure-natriuresis relationship in transgenic rats with inducible angiotensin II (ANG II)-dependent hypertension (Cyp1a1-Ren-2 rats). METHODS: The renin gene was induced in Cyp1a1-Ren-2 rats through dietary administration of the natural xenobiotic indole-3-carbinol (I3C, 0.3%) for 12 and 24 h, respectively. Noninduced rats served as controls. Anesthetized rats were prepared for renal function studies and an aortic clamp was placed above the junction of the left renal artery to regulate the level of renal arterial pressure. Plasma renin activity, ANG II and aldosterone levels were measured at the end of the experiment by radioimmunoassay. RESULTS: Administration of I3C resulted in progressive increases in plasma renin activity and plasma and kidney ANG II levels; however, it did not significantly alter aldosterone levels as compared with those in noninduced rats. I3C induction for 12 h did not cause significant changes in blood pressure as compared with those in noninduced rats. I3C induction for 24 h elicited a significant rise in blood pressure. Twelve-hour I3C induction caused an impairment of the autoregulatory efficiency of renal blood flow and glomerular filtration rate and of the pressure-natriuresis relationship as compared with that in noninduced rats. In addition, 24 h I3C induction of the renin gene resulted in a marked reduction in renal blood flow and glomerular filtration rate and a further impairment of the pressure-natriuresis mechanism as compared with that in noninduced rats. CONCLUSION: Our findings indicate that an impairment of the pressure-natriuresis mechanism precedes the development of ANG II-dependent hypertension in Cyp1a1-Ren-2 transgenic rats.
References provided by Crossref.org
Research on Experimental Hypertension in Prague (1966-2009)
