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PubMed

Záznam pochází z PubMed

Fibroblasts prepared from different types of malignant tumors stimulate expression of luminal marker keratin 8 in the EM-G3 breast cancer cell line

Histochemistry and cell biology. 2012 May ; 137 (5) : 679-85. [epub] 20120124

Histochem Cell Biol
ISSN 1432-119X | 0948-6143
Zdroj

Jazyk angličtina Země Německo Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz https://www.medvik.cz/link/pmid22270320

PubMed 22270320
DOI 10.1007/s00418-012-0918-3
Knihovny.cz E-zdroje

MeSH
bazocelulární karcinom metabolismus patologie MeSH
buňky 3T3 MeSH
fibroblasty metabolismus MeSH
keratin-8 metabolismus MeSH
kokultivační techniky MeSH
lidé MeSH
melanom metabolismus patologie MeSH
myši MeSH
nádorové biomarkery metabolismus MeSH
nádorové buněčné linie MeSH
nádory kůže metabolismus patologie sekundární MeSH
nádory prsu metabolismus patologie MeSH
spinocelulární karcinom metabolismus patologie MeSH
zvířata MeSH
Check Tag
lidé MeSH
myši MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
keratin-8 MeSH
KRT8 protein, human MeSH Prohlížeč
nádorové biomarkery MeSH

It is widely recognized that stromal fibroblasts significantly influence biological properties of multiple tumors including breast cancer. However, these epithelial-mesenchymal interactions seem to be essential in tumor biology and it is not fully clear whether this interaction is tumor type-specific or has a more general non-specific character. To elucidate this question, we tested the effect of cancer-associated fibroblasts (CAFs) isolated from different types of tumors (breast cancer skin metastasis, cutaneous basal cell carcinoma and melanoma, squamous cell carcinoma arising from oral cavity mucous membrane) on the EM-G3 breast cancer cell line. The results were compared with control experiments using normal human dermal fibroblasts, 3T3 mouse fibroblasts, and 3T3 fibroblasts influenced by the fibroblasts prepared from the basal cell carcinoma. Our results demonstrated that expression of luminal marker keratin 8 was influenced only by CAFs prepared from any tested tumors. In contrast, all tested types of fibroblasts showed a strong stimulatory effect on the expression of basal/myoepithelial marker keratin 14. The CAFs also elevated the number of cells with positivity for both keratins 8 and 14 that are similar to ductal originated precursor cells. The expression of proliferation marker Ki67 was not influenced by any of the tested fibroblasts. In conclusion, our data indicate that CAFs are able to influence the phenotype of a breast cancer cell line and this effect is based on a tumor type-unspecific mechanism. Finally, a clear functional difference between normal and CAFs was demonstrated.

Institute of Anatomy Charles University Prague Czech Republic

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