Therapy for pancreatic ductal adenocarcinoma remains challenging, and the chances of a complete cure are very limited. As in other types of cancer, the expression and role of miRNAs in controlling the biological properties of this type of tumor have been extensively studied. A better insight into miRNA biology seems critical to refining diagnostics and improving their therapeutic potential. In this study, we focused on the expression of miR-21, -96, -196a, -210, and -217 in normal fibroblasts, cancer-associated fibroblasts prepared from a ductal adenocarcinoma of the pancreas, and pancreatic carcinoma cell lines. We compared these data with miRNAs in homogenates of paraffin-embedded sections from normal pancreatic tissues. In cancer-associated fibroblasts and cancer cell lines, miRNAs differed significantly from the normal tissue. In detail, miR-21 and -210 were significantly upregulated, while miR-217 was downregulated. Similar transcription profiles were earlier reported in cancer-associated fibroblasts exposed to hypoxia. However, the cells in our study were cultured under normoxic conditions. We also noted a relation to IL-6 production. In conclusion, cultured cancer-associated fibroblasts and carcinoma cells reflect miR-21 and -210 expression similarly to the cancer tissue samples harvested from the patients.

1st Faculty of Medicine BIOCEV Charles University 252 50 Vestec Czech Republic

1st Faculty of Medicine Institute of Anatomy Charles University 128 00 Prague Czech Republic

Department Dermatovenereology 1st Faculty of Medicine Charles University and General University Hospital 128 08 Prague Czech Republic

Department of Medical Biophysics and Medical Informatics 3rd Faculty of Medicine Charles University 100 00 Prague Czech Republic

Department of Pathology 3rd Faculty of Medicine Charles University and University Hospital Královské Vinohrady 100 00 Prague Czech Republic

Department of Surgery 3rd Faculty of Medicine Charles University and University Hospital Královské Vinohrady 100 00 Prague Czech Republic

Institute of Molecular Genetics Czech Academy of Sciences 100 00 Prague Czech Republic

Laboratory of Informatics and Chemistry University of Chemistry and Technology 166 28 Prague Czech Republic

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Wound healing: insights into autoimmunity, ageing, and cancer ecosystems through inflammation and IL-6 modulation