Magnetic resonance imaging (MRI) using 19F-based tracers has emerged as a promising multi-purpose noninvasive diagnostic tool and its application requires the use of various 19F-based tracers for the intended diagnostic purpose. In this study, we report a series of double-stimuli-responsive polymers for use as injectable implants, which were designed to form implants under physiological conditions, and to subsequently dissolve with different dissolution rates (t1/2 ranges from 30 to more than 250 days). Our polymers contain a high concentration of fluorine atoms, providing remarkable signal detectability, and both a hydrophilic monomer and a pH-responsive monomer that alter the biodistribution properties of the implant. The implant location and dissolution were observed using 19F MRI, which allows the anatomic extent of the implant to be monitored. The dissolution kinetics and biocompatibility of these materials were thoroughly analyzed. No sign of toxicity in vitro or in vivo or pathology in vivo was observed, even in chronic administration. The clinical applicability of our polymers was further confirmed via imaging of a rat model by employing an instrument currently used in human medicine.

Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine Videnska 1958 9 140 21 Prague 4 Czech Republic

Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine Videnska 1958 9 140 21 Prague 4 Czech Republic; Department of Science and Research Faculty of Health Studies Technical University of Liberec Studentska 1402 2 461 17 Liberec Czech Republic

Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine Videnska 1958 9 140 21 Prague 4 Czech Republic; Institute of Biophysics and Informatics Charles University 1st Faculty of Medicine Salmovská 1 120 00 Prague 2 Czech Republic

Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine Videnska 1958 9 140 21 Prague 4 Czech Republic; Technical University of Liberec Faculty of Mechatronics Informatics and Interdisciplinary Studies Studentska 1402 2 461 17 Liberec Czech Republic

Department of Organic and Macromolecular Chemistry Ghent University Krijgslaan 281 S4 9000 Ghent Belgium

Institute of Macromolecular Chemistry CAS Heyrovsky Square 2 162 06 Prague 6 Czech Republic

Institute of Macromolecular Chemistry CAS Heyrovsky Square 2 162 06 Prague 6 Czech Republic; Department of Organic Chemistry Charles University Faculty of Science Hlavova 8 128 43 Prague 2 Czech Republic; Institute of Biophysics and Informatics Charles University 1st Faculty of Medicine Salmovská 1 120 00 Prague 2 Czech Republic; Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Flemingovo square 542 2 162 06 Prague 6 Czech Republic

Institute of Macromolecular Chemistry CAS Heyrovsky Square 2 162 06 Prague 6 Czech Republic; Department of Physical and Macromolecular Chemistry Faculty of Science Charles University Hlavova 8 Prague 2 128 00 Czech Republic

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