Satellite DNAs and the evolution of the multiple X1X2Y sex chromosomes in the wolf fish Hoplias malabaricus (Teleostei; Characiformes)
Language English Country Great Britain, England Media electronic
Document type Journal Article
Grant support
2022/00427-3
Fundação de Amparo à Pesquisa do Estado de São Paulo
2023/00955-2
Fundação de Amparo à Pesquisa do Estado de São Paulo
302928/2021-9
Conselho Nacional de Desenvolvimento Científico e Tecnológico
PubMed
39223262
PubMed Central
PMC11369246
DOI
10.1038/s41598-024-70920-7
PII: 10.1038/s41598-024-70920-7
Knihovny.cz E-resources
- Keywords
- FISH, Meiosis, Multiple sex chromosomes, Satellitome, Sex trivalent,
- MeSH
- Characiformes * genetics MeSH
- Y Chromosome genetics MeSH
- In Situ Hybridization, Fluorescence * MeSH
- Karyotype MeSH
- Meiosis genetics MeSH
- Evolution, Molecular MeSH
- Sex Chromosomes * genetics MeSH
- DNA, Satellite * genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Satellite * MeSH
Multiple sex chromosomes usually arise from chromosomal rearrangements which involve ancestral sex chromosomes. There is a fundamental condition to be met for their long-term fixation: the meiosis must function, leading to the stability of the emerged system, mainly concerning the segregation of the sex multivalent. Here, we sought to analyze the degree of differentiation and meiotic pairing properties in the selected fish multiple sex chromosome system present in the wolf-fish Hoplias malabaricus (HMA). This species complex encompasses seven known karyotype forms (karyomorphs) where the karyomorph C (HMA-C) exhibits a nascent XY sex chromosomes from which the multiple X1X2Y system evolved in karyomorph HMA-D via a Y-autosome fusion. We combined genomic and cytogenetic approaches to analyze the satellite DNA (satDNA) content in the genome of HMA-D karyomorph and to investigate its potential contribution to X1X2Y sex chromosome differentiation. We revealed 56 satDNA monomers of which the majority was AT-rich and with repeat units longer than 100 bp. Seven out of 18 satDNA families chosen for chromosomal mapping by fluorescence in situ hybridization (FISH) formed detectable accumulation in at least one of the three sex chromosomes (X1, X2 and neo-Y). Nine satDNA monomers showed only two hybridization signals limited to HMA-D autosomes, and the two remaining ones provided no visible FISH signals. Out of seven satDNAs located on the HMA-D sex chromosomes, five mapped also to XY chromosomes of HMA-C. We showed that after the autosome-Y fusion event, the neo-Y chromosome has not substantially accumulated or eliminated satDNA sequences except for minor changes in the centromere-proximal region. Finally, based on the obtained FISHpatterns, we speculate on the possible contribution of satDNA to sex trivalent pairing and segregation.
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