Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články
Grantová podpora
DP2 GM132931
NIGMS NIH HHS - United States
R01 GM145916
NIGMS NIH HHS - United States
PubMed
39835937
PubMed Central
PMC11749194
DOI
10.1039/d5tb00074b
Knihovny.cz E-zdroje
- MeSH
- aktiny metabolismus chemie MeSH
- cytoskelet metabolismus účinky léků MeSH
- DNA * chemie metabolismus MeSH
- lidé MeSH
- mikrofilamenta metabolismus účinky léků MeSH
- nanostruktury * chemie MeSH
- velikost částic MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aktiny MeSH
- DNA * MeSH
DNA nanostructures (DNs) have gained popularity in various biomedical applications due to their unique properties, including structural programmability, ease of synthesis and functionalization, and low cytotoxicity. Effective utilization of DNs in biomedical applications requires a fundamental understanding of their interactions with living cells and the mechanics of cellular uptake. Current knowledge primarily focuses on how the physicochemical properties of DNs, such as mass, shape, size, and surface functionalization, affect uptake efficacy. However, the role of cellular mechanics and morphology in DN uptake remains largely unexplored. In this work, we show that cells subjected to geometric constraints remodel their actin cytoskeleton, resulting in differential mechanical force generation that facilitates DN uptake. The length, number, and orientation of F-actin fibers are influenced by these constraints, leading to distinct mechanophenotypes. Overall, DN uptake is governed by F-actin forces arising from filament reorganisation under geometric constraints. These results underscore the importance of actin dynamics in the cellular uptake of DNs and suggest that leveraging geometric constraints to induce specific cell morphology adaptations could enhance the uptake of therapeutically designed DNs.
Biodesign Center for Molecular Design and Biomimetics Arizona State University Tempe USA
Faculty of Mathematics and Physics Charles University Ke Karlovu 3 CZ 121 16 Prague 2 Czech Republic
Institute for Clinical and Experimental Medicine Prague 14021 Czech Republic
School of Molecular Sciences Arizona State University Tempe Arizona 85287 USA
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