- O autorovi
- Waisser, Karel, 1936-2017 Autorita
Equipment for fast and accurate detection of organophosphate nerve agents is developed and tested. The method is based on the spectrophotometric monitoring of the enzyme activity of butyrylcholinesterase after its contact with air in a special absorption unit (a “scrubber”) developed for the purpose. The scrubber was made from a glass tube filled with glass beads (diam. 3 mm) and filled with approx. 5 ml of butyrylcholinesterase in a phosphate buffer of pH 7.4. The air sample was bubbled through this solution for 20 s at a flow rate of 80 l hour-1. Thereafter 8 microl of the enzyme solution were aspirated into the micro-SIA-LOV analyzer and the activity of the enzymes were evaluated by using Ellman’s reagent, i.e. 2.5 mmol l-1 butyrylthiocholine iodide and 0.25 mmol 5,5’-dithiobis (2-nitrobenzoic acid). The absorbance of the coloured reaction product was measured at 412 nm after the reaction time of 60 s. The residue of the absorption liquid was washed away from the absorber and the system was washed with the enzyme solution prior to next analysis. The contaminated air caused partial inhibition of the enzyme activity of the absorption liquid. The activity of the contaminated sample was compared with the activity of the unaffected enzyme (blank measurement). The analysis was controlled by two PCs. The effect of the concentration of analyte in the absorption liquid on the enzyme activity was tested for 10-5-10-9 mol l-1 sarin. A single analysis (including the absorption step) took <130 s.
- MeSH
- butyrylcholinesterasa MeSH
- chemické bojové látky MeSH
- cholinesterasové inhibitory analýza MeSH
- design vybavení MeSH
- laboratorní automatizace přístrojové vybavení MeSH
- organofosforové sloučeniny analýza MeSH
- průtoková injekční analýza * metody přístrojové vybavení statistika a číselné údaje MeSH
- sarin * analýza MeSH
- sekvenční analýza metody MeSH
- spektrofotometrie metody MeSH
- zdravotnické prostředky MeSH
- znečištění ovzduší * analýza statistika a číselné údaje MeSH
- Publikační typ
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
AIMS: We are currently witnessing changes in views on the evaluation of serum proteins. A decrease may signal not only malnutrition. It may also be an indicator of simultaneously occurring inflammatory disease. Prealbumin, due to its short half-life, is a suitable indicator of changes in protein-energy balance, but its levels show, as with other serum proteins, a decrease in the case of inflammation too. The present study aimed to determine the prealbumin values of hospitalized geriatric patients and how they are affected by inflammatory disease. METHODS: In 101 patients aged over 80 years, the relationships were compared between prealbumin and C-reactive protein in the whole group and then in the subgroups with normal and increased C-reactive protein. RESULTS: In 67.33 % of hospitalized geriatric patients prealbumin was below the limit of the norm. A statistically highly significant dependence (p < 0.001) was demonstrated between a decrease in prealbumin and an increase in C-reactive protein in the whole group. In the subgroup with normal C-reactive protein, no statistically significant decrease in prealbumin was demonstrated, whereas in the subgroup with increased C-reactive protein a significant decrease in prealbumin (p < 0.001 for the whole group, p < 0.01 men, p < 0.05 women) was found. CONCLUSIONS: The study demonstrated subnormal mean initial values of prealbumin and a highly statistically significant negative correlation between a decrease in prealbumin and an increase in C-reactive protein in the whole group. We confirm that in inflammation there is a statistically significant decrease in serum concentration of prealbumin.
- MeSH
- biologické markery krev MeSH
- C-reaktivní protein analýza MeSH
- hospitalizace MeSH
- lidé MeSH
- podvýživa diagnóza MeSH
- prealbumin analýza MeSH
- senioři nad 80 let MeSH
- zánět krev diagnóza MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- antinematodní látky farmakologie MeSH
- cytochrom P-450 CYP1A1 metabolismus účinky léků MeSH
- financování organizované MeSH
- Galliformes metabolismus MeSH
- hepatocyty enzymologie MeSH
- mebendazol analogy a deriváty farmakologie MeSH
- oxidoreduktasy účinky léků MeSH
- systém (enzymů) cytochromů P-450 účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- anthelmintika farmakologie MeSH
- cytochrom P-450 CYP1A1 metabolismus MeSH
- fenbendazol farmakologie MeSH
- finanční podpora výzkumu jako téma MeSH
- játra účinky léků MeSH
- mebendazol analogy a deriváty farmakologie MeSH
- prasata MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
