This study is focussed on micro-encapsulation of essential oils in polylactic acid (PLA) and a poly(methyl methacrylate) (PMMA) matrix as well as blends of the same. Microspheres were prepared by the solvent evaporation technique and characterised by scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infra-red spectroscopy (FTIR). The encapsulation efficiencies and release profiles of the essential oils were studied by gas chromatography mass spectrometry (GC-MS) and head-space solid-phase microextraction GC-MS, respectively. Furthermore, the microspheres were tested for antibacterial activity against both Gram-negative and Gram-positive bacterial strains. The results showed that the microspheres compositions (PLA/PMMA ratio) have significant effect on their characteristics. The process adopted for preparing the microspheres promoted formation of spherical particles at the sizes of 1.5-9.5 µm. The highest encapsulation efficiency of the prepared microspheres was observed in systems consisting of linalool (81.10 ± 10.0 wt. % for PLA system and 76.0 ± 3.3 wt. % for PMMA system). Confirmation was also made that the release rate of the microspheres was affected by the size of the same.
- MeSH
- antibakteriální látky aplikace a dávkování farmakologie MeSH
- Bacteria účinky léků MeSH
- bakteriální infekce farmakoterapie MeSH
- lidé MeSH
- nosiče léků chemie MeSH
- oleje prchavé aplikace a dávkování farmakologie MeSH
- polyestery chemie MeSH
- polymethylmethakrylát chemie MeSH
- příprava léků MeSH
- velikost částic MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
This work is focused on preparation of novel porous type of core-shell-structured microparticles based on polylactide (shell) and poly(vinyl alcohol) cross-linked with glutaric acid (GA) (core) prepared by water-in-oil-in-water solvent evaporation technique. The microparticle systems were used as delivery systems for immobilisation of model antibacterial agent - nisin. The effect of cross-linking and the initial amount of nisin on their morphology was investigated using scanning electron microscopy, BET analysis, zeta potential measurement and Fourier transform infra-red spectroscopy. Encapsulation efficiency and release profile of nisin from the microparticles were studied by high performance liquid chromatography. Antibacterial activity of the prepared systems was tested by dilution and spread plate technique. Results showed the microparticles in the size range of 9-16 μm in diameter with spherical multi-hollow core-shell structure. The presence of cross-linking agent GA influences the release profile of the peptide and has synergistic effect on Listeria monocytogenes growth reduction.
- MeSH
- antibakteriální látky aplikace a dávkování MeSH
- glutaráty chemie MeSH
- Listeria monocytogenes účinky léků MeSH
- mikroskopie elektronová rastrovací MeSH
- nisin aplikace a dávkování MeSH
- nosiče léků chemie MeSH
- polyestery chemie MeSH
- polyvinylalkohol chemie MeSH
- poréznost MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
