Oilseed cakes and meals are obtained as by-products after oil extraction. They contain 15–60 % proteins with a relatively good amino acid proportion and other valuable components such as dietary fibre, residual fat, polyphenols etc. Proteins are above all represented by globulins, especially 11-12S globulin, and albumins. Oilseed cakes are often used as feed for farm animals but they can be valorised in the form of flour and protein concentrate or by an isolation using dry or wet processes. In this work, a characterization of seed proteins of selected oilseed crops and methods of protein isolation are presented. Possibilities of oilseed protein products use in human nutrition and food products are evaluated.
Biochemical characteristics of patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with respect to utility groups, table potato cultivars (TPCs) and processing potato cultivars (PPCs). Both groups of cultivars showed similar values of protein content in dry matter (3.98-7.39%) and of patatin relative abundance (5.40-35.40%). Three mass levels (∼40.6, 41.8, and 42.9 kDa) of purified patatins were found by MALDI-TOF MS within all cultivars. Differences among mass levels corresponding with the mass of sugar antenna (∼1.2 kDa) confirmed the previous concept of different glycosylation extentsin patatin proteins. It was showed that the individual types of patatin varying in their masses occur in the patatin family in a ratio specific for each of the cultivars, with the lowest mass type being the major one. Electrophoretic analyses demonstrated wide cultivar variability in number of patatin forms. Especially 2D-PAGE showed 17-23 detected protein spots independently on the utility group. Specific lipid acyl hydrolase (LAH) activity of purified patatins from the individual tested cultivars varied between 0.92 and 5.46 μmol/(min mg). Patatin samples within most of the TPCs exhibited higher values of specific LAH activity than samples of PPCs. It may be supposed that individual patatin forms do not have similar physiological roles.
- MeSH
- druhová specificita MeSH
- glykosylace MeSH
- hlízy rostlin chemie MeSH
- karboxylesterhydrolasy chemie izolace a purifikace metabolismus MeSH
- molekulová hmotnost MeSH
- protein - isoformy chemie izolace a purifikace metabolismus MeSH
- rostlinné proteiny analýza chemie izolace a purifikace metabolismus MeSH
- Solanum tuberosum chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Two methods recommended for analysis of denatured proteins, automated Experion chip electrophoresis and SDS-PAGE, are compared. The Experion method is a novel technique based on combination of the LabChip microfluidic separation technique (Caliper Life Sciences) and sensitive fluorescent detection. Both methods were compared in molecular weight (MW) determination of a protein standard mixture, resolution of protein pairs of near molecular weights and estimation of the abundance of a target protein in the mixture to be purified. Both the methods are appropriate for MW determination and in purification of proteins. The accuracy of the methods is approximately the same (ca. 8 %), but the Experion method shows better reproducibility (ca. 1.48 %) than SDS-PAGE (ca. 2.17 %).
- MeSH
- lidé MeSH
- neziskové organizace organizace a řízení MeSH
- pomoc v nouzi MeSH
- sociální práce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- rozhovory MeSH
A new modular methodology of preparation of 5-substituted thiophene-2-yl C-nucleosides was developed. A Friedel-Crafts-type of C-glycosidation of 2-bromothiophene with toluoyl-protected methylglycoside 2 gave the desired protected 1beta-(5-bromothiophen-2-yl)-1,2-dideoxyribofuranose 4a in 60%. The key intermediate 4a was then subjected to a series of palladium-catalyzed cross-coupling reactions. The cross-coupling reactions with alkyl organometallics gave beta-(5-alkylthiophen-2-yl)-2-deoxyribonucleosides 4 and 7 in moderate yields accompanied by side-products of reduction. On the other hand, cross-couplings with arylstannanes proceeded smoothly to give a series of beta-(5-arylthiophen-2-yl)-2-deoxyribonucleosides 4 in good yields. Deprotection of toluoylated nucleosides by NaOMe in MeOH and silylated nucleosides by Et 3N.3HF gave a series of free C-nucleosides 6. Alternatively, other types of 5-arylthiophene C-nucleosides 6 were prepared in one step by the aqueous-phase cross-coupling reactions of unprotected 1beta-(5-bromothiophen-2-yl)-1,2-dideoxyribofuranose with boronic acids. Title 5-arylthiophene C-nucleosides 6 exhibit interesting fluorescent properties with emission maxima varying from 339 to 396 nm depending on the aryl group attached.
1. vyd. 358 s. : il. ; 21 cm
Spolehlivý rádce, který podá pomocnou ruku při zápolení s taji a záludnostmi francouzského jazyka. Nabízí ucelený přehled francouzských gramatických jevů.
- Klíčová slova
- Francouzština,
- MeSH
- lingvistika MeSH
- Publikační typ
- příručky MeSH
- Konspekt
- Francouzština
- NLK Obory
- lingvistika, lékařská terminologie
