Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UCP2-positive cells in liver and inside primitive blood vessels from 10.5 days of prenatal development. High UCP2 transcript levels were detected in reticulocytes and other maturating erythroid cells in peripheral blood of mice exposed to hypoxia, and in umbilical cord blood of human neonates and peripheral blood of adults. Our results suggest involvement of UCP2 in erythropoiesis.

• MeSH
• embryo savčí MeSH
• erytroidní buňky cytologie   chemie   MeSH
• erytropoéza genetika   MeSH
• fetální krev MeSH
• játra cytologie   MeSH
• lidé MeSH
• messenger RNA analýza   MeSH
• myši MeSH
• proteiny analýza   fyziologie   genetika   MeSH
• retikulocyty chemie   MeSH
• stárnutí buněk genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

AIMS/HYPOTHESIS: Intake of n-3 polyunsaturated fatty acids reduces adipose tissue mass, preferentially in the abdomen. The more pronounced effect of marine-derived eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids on adiposity, compared with their precursor alpha-linolenic acid, may be mediated by changes in gene expression and metabolism in white fat. METHODS: The effects of EPA/DHA concentrate (6% EPA, 51% DHA) admixed to form two types of high-fat diet were studied in C57BL/6J mice. Oligonucleotide microarrays, cDNA PCR subtraction and quantitative real-time RT-PCR were used to characterise gene expression. Mitochondrial proteins were quantified using immunoblots. Fatty acid oxidation and synthesis were measured in adipose tissue fragments. RESULTS: Expression screens revealed upregulation of genes for mitochondrial proteins, predominantly in epididymal fat when EPA/DHA concentrate was admixed to a semisynthetic high-fat diet rich in alpha-linolenic acid. This was associated with a three-fold stimulation of the expression of genes encoding regulatory factors for mitochondrial biogenesis and oxidative metabolism (peroxisome proliferator-activated receptor gamma coactivator 1 alpha [Ppargc1a, also known as Pgc1alpha] and nuclear respiratory factor-1 [Nrf1] respectively). Expression of genes for carnitine palmitoyltransferase 1A and fatty acid oxidation was increased in epididymal but not subcutaneous fat. In the former depot, lipogenesis was depressed. Similar changes in adipose gene expression were detected after replacement of as little as 15% of lipids in the composite high-fat diet with EPA/DHA concentrate, while the development of obesity was reduced. The expression of Ppargc1a and Nrf1 was also stimulated by n-3 polyunsaturated fatty acids in 3T3-L1 cells. CONCLUSIONS/INTERPRETATION: The anti-adipogenic effect of EPA/DHA may involve a metabolic switch in adipocytes that includes enhancement of beta-oxidation and upregulation of mitochondrial biogenesis.

• MeSH
• epididymis metabolismus   účinky léků   MeSH
• faktor 1 související s NF-E2 genetika   účinky léků   MeSH
• karnitin-O-palmitoyltransferasa genetika   účinky léků   MeSH
• kultivované buňky MeSH
• kyselina alfa-linolenová farmakologie   MeSH
• kyselina eikosapentaenová farmakologie   MeSH
• kyseliny dokosahexaenové farmakologie   MeSH
• lipogeneze účinky záření   MeSH
• mitochondriální proteiny metabolismus   účinky léků   MeSH
• mitochondrie metabolismus   účinky léků   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nenasycené mastné kyseliny farmakologie   izolace a purifikace   metabolismus   MeSH
• obezita prevence a kontrola   MeSH
• oxidace-redukce MeSH
• podkožní tuk metabolismus   účinky léků   MeSH
• regulace genové exprese účinky záření   MeSH
• rybí oleje chemie   MeSH
• trans-aktivátory genetika   účinky léků   MeSH
• tuková tkáň metabolismus   účinky léků   MeSH
• tukové buňky metabolismus   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• lipolýza genetika   MeSH
• myši MeSH
• noradrenalin metabolismus   MeSH
• proteiny vázající GTP metabolismus   MeSH
• transportní proteiny fyziologie   MeSH
• tuková tkáň metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH

• MeSH
• energetický metabolismus MeSH
• inzulinová rezistence MeSH
• lidé MeSH
• lipidy genetika   MeSH
• metabolismus lipidů MeSH
• obezita genetika   prevence a kontrola   MeSH
• transportní proteiny genetika   MeSH
• tukové buňky MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH

• MeSH
• HIV infekce farmakoterapie   MeSH
• HIV-1 účinky léků   MeSH
• lidé MeSH
• oligopeptidy farmakologie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Resistant mutants of the HIV protease from the blood lymphocytes of AIDS patients treated with protease inhibitors will be amplified, sequenced, characterized in vitro and in tissue culture and tested with a panel of structurally different inhibitors.

Z lymfocytů pacientů s AIDS léčených inhibitory proteasy HIV budou sekvenovány a charakterisovány in vitro i ve tkáňové kultuře mutanty proteasy, resistentní vůči původnímu inhibitoru, a otestovány pomocí panelu struktuálně rozdílných inhibitorů.

• MeSH
• AIDS terapie   MeSH
• antibiotická rezistence MeSH
• molekulární modely MeSH
• molekulární struktura MeSH

• Konspekt
• Virologie
• NLK Obory
• dermatovenerologie
• biologie
• virologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR