The increasing worldwide production of bisphenols has been associated to several human diseases, such as chronic respiratory and kidney diseases, diabetes, breast cancer, prostate cancer, behavioral troubles and reproductive disorders in both sexes. The aim of the present in vitro study was to evaluate the potential impact bisphenols A, B, S and F on the cell viability and testosterone release in TM3 Leydig cell line. Mice Leydig cells were cultured in the presence of different concentrations of bisphenols (0.04-50 µg.ml-1) during 24 h exposure. Quantification of the cell viability was assessed using the metabolic activity assay, while the level of testosterone in cell culture media was determined by enzyme-linked immunosorbent assay. Within the panel of substances under investigations, the higher experimental concentrations (10; 25 and 50 µg.ml-1) significantly (P<0.001) decreased Leydig cells viability, while the same doses of BPA and BPB also reduced testosterone production significantly (P<0.001). Taken together, the results of our study reported herein is a consistent whit the conclusion that higher experimental doses of bisphenols have a cytotoxic effect and could have a dose-dependent impact on testosterone production.
- MeSH
- benzhydrylové sloučeniny aplikace a dávkování toxicita MeSH
- buněčné linie MeSH
- endokrinní disruptory aplikace a dávkování toxicita MeSH
- estrogeny nesteroidní aplikace a dávkování toxicita MeSH
- fenoly aplikace a dávkování toxicita MeSH
- Leydigovy buňky metabolismus účinky léků MeSH
- mitochondrie metabolismus účinky léků MeSH
- myši MeSH
- testosteron antagonisté a inhibitory metabolismus MeSH
- viabilita buněk fyziologie účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Antimicrobial and immunomodulatory potential of various Lactobacillus reuteri strains is closely connected to their metabolite production profile under given cultivation conditions. We determined the in vitro production of antimicrobial substances such as organic acids, ethanol, and reuterin by four strains of L. reuteri (L. reuteri E, L. reuteri KO5, L. reuteri CCM 3625, and L. reuteri ATCC 55730). All studied L. reuteri strains showed the ability to produce lactic acid, acetic acid, and ethanol with concominant consumption of glucose and together with phenyllactic acid-a potent antifungal compound-with concominant consumption of phenylalanine. The reuterin production from glycerol was confirmed for all analyzed lactobacilli strains except L. reuteri CCM 3625. Production of organic acids, ethanol, and reuterin is significantly involved in antimicrobial activity of lactobacilli which was determined using the dual-culture overlay diffusion method against six indicator bacteria and five indicator moulds. In comparison to the referential L. reuteri ATCC 55730, the highest inhibition potential was observed against Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 3955. Among analyzed indicators of moulds, the growth of Alternaria alternata CCM F-128 was the most inhibited by all four analyzed L. reuteri strains. Finally, the immunomodulatory potential of analyzed lactobacilli were proven by the determination of the in vitro production of biogenic amines histamine and tyramine. L. reuteri CCM 3625 was able to produce tyramine, and L. reuteri E and L. reuteri KO5 were able to produce histamine under given cultivation conditions.
- MeSH
- antibakteriální látky analýza metabolismus farmakologie MeSH
- Escherichia coli účinky léků MeSH
- fermentace MeSH
- imunologické faktory analýza metabolismus farmakologie MeSH
- kyselina mléčná analýza metabolismus farmakologie MeSH
- kyselina octová analýza metabolismus farmakologie MeSH
- Limosilactobacillus reuteri chemie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
The suitability of lactic acid bacteria (Lactobacillus planatarum CCM 7039, Lactobacillus amylovorus CCM 4380) as starter culture for oat beverage fermentation was investigated. The fermentation was carried out at 37 °C for 36 h. The counts of lactic acid bacteria reached 8.55 log CFU cm–3 after 24 h fermentation, from an initial concentration of log 7.23 CFU cm–3 and pH decreased from 7.4 to below 3.8. The oat beverage inoculated with Lactobacillus plantarum CCM 7039 showed higher values of sensory parameters than oat beverage inoculated with Lactobacillus amylovorus CCM 4380. The oat-based substrate proved to be a suitable medium for the tested Lactobacillus strains.
- MeSH
- finanční podpora výzkumu jako téma MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- Bifidobacterium chemie MeSH
- fermentace MeSH
- finanční podpora výzkumu jako téma MeSH
- Lactobacillus chemie MeSH
- probiotika chemie MeSH
- Publikační typ
- kongresy MeSH
