- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
SCOPE: The major alimentary sources for the plasma membrane lipid sphingomyelin (SM) are dairy products, eggs, and meat. We recently reported that the SM metabolite ceramide induces cathepsin D mediated apoptosis in murine intestinal epithelial cells (IECs) and increases inflammation in acute colitis. We investigated the impact of SM and phosphatidylcholine on apoptosis in human IECs and point out BH3-interacting death agonist (BID) as link between cathepsin D and apoptosis. METHODS AND RESULTS: HT-29 and isolated human IECs were stimulated with SM or phosphatidylcholine. SM treatment resulted in increased apoptosis. Phosphatidylcholine showed contrary effects. Western revealed higher amounts of cathepsin D and BID activation upon lipid stimulation. Western blotting revealed BID activation through SM in both an induced and a spontaneous mouse model of colitis. CONCLUSION: Dietary phospholipids may induce or abolish apoptosis in IECs and seem to play a role in the pathogenesis of inflammatory bowel diseases. This nutritional factor might be considered when evaluating the pathogenesis of inflammatory bowel diseases. Effects of SMase- and SM treatment on inflammation in dextran sulfate sodium induced animal models of colitis and in vitro experiments are discussed as controversial. Variable sources of SM, feeding techniques, and mouse strains might play a role.
- MeSH
- adhezní spoje MeSH
- apoptóza * účinky léků MeSH
- buněčná smrt účinky léků MeSH
- buňky HT-29 účinky léků MeSH
- ceramidy metabolismus MeSH
- epitelové buňky * patologie účinky léků MeSH
- fosfatidylcholiny farmakologie metabolismus MeSH
- kathepsin D metabolismus MeSH
- kolitida metabolismus patologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- liposomy farmakologie MeSH
- myši inbrední C57BL MeSH
- potravní doplňky MeSH
- protein Bid metabolismus MeSH
- sfingomyelinfosfodiesterasa metabolismus MeSH
- sfingomyeliny farmakologie metabolismus MeSH
- střeva * cytologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
U většiny pacientů s morbidní obezitou (BMI >40 kg/m2) je obtížné dosáhnout úspěšného snížení tělesné hmotnosti a u většiny z nich je to při použití neinvazivních postupů nemožné. Pro tyto pacienty je chirurgická léčba účinnou alternativou. Případný chirurgický zásah musí být ale během šestiměsíční předoperační fáze pečlivě zvážen. V tomto období je nutné posoudit indikace a kontraindikace zásahu.
A successful reduction of body weight in patients with morbid obesity (BMI >40 kg/m2) is difficult and in the majority of patients it is impossible with non-interventional treatment modalities. Surgical therapy is an efficient alternative for these patients. Potential surgical intervention, however, should be carefully evaluated during a six month preoperative treatment phase. During this time period indications and contraindications must be evaluated.
- MeSH
- bariatrie metody MeSH
- bazální metabolismus fyziologie MeSH
- dietoterapie metody využití MeSH
- lidé MeSH
- morbidní obezita chirurgie komplikace terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
