Stem cells biology is one of the most frequent topic of physiological research of today. Spinal fusion represents common bone biology challenge. It is the indicator of osteoinduction and new bone formation on ectopic model. The purpose of this study was to establish a simple model of spinal fusion based on a rat model including verification of the possible use of titanium microplates with hydroxyapatite scaffold combined with human bone marrow-derived mesenchymal stem cells (MSCs). Spinous processes of two adjacent vertebrae were fixed in 15 Wistar rats. The space between bony vertebral arches and spinous processes was either filled with augmentation material only and covered with a resorbable collagen membrane (Group 1), or filled with augmentation material loaded with 5 × 10⁶ MSCs and covered with a resorbable collagen membrane (Group 2). The rats were sacrificed 8 weeks after the surgery. Histology, histomorphometry and micro-CT were performed. The new model of interspinous fusion was safe, easy, inexpensive, with zero mortality. We did not detect any substantial pathological changes or tumor formation after graft implantation. We observed a nonsignificant effect on the formation of new bone tissue between Group 1 and Group 2. In the group with MSCs (Group 2) we described minor inflamatory response which indicates the imunomodulational and antiinflamatory role of MSCs. In conclusion, this new model proved to be easy to use in small animals like rats.
- MeSH
- bederní obratle patofyziologie radiografie chirurgie MeSH
- časové faktory MeSH
- fúze páteře škodlivé účinky přístrojové vybavení metody MeSH
- hydroxyapatit MeSH
- kostní destičky MeSH
- kultivované buňky MeSH
- lidé MeSH
- modely u zvířat MeSH
- osteogeneze MeSH
- osteointegrace MeSH
- potkani Wistar MeSH
- protézy - design MeSH
- regenerace * MeSH
- rentgenová mikrotomografie MeSH
- titan MeSH
- tkáňové podpůrné struktury MeSH
- transplantace mezenchymálních kmenových buněk škodlivé účinky přístrojové vybavení metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
PURPOSE: Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC(®)) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects. METHODS: Under general isoflurane anesthesia, a defect in the body of the L2 vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed. RESULTS: We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation. CONCLUSIONS: MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.
- MeSH
- bederní obratle zranění patologie radiografie MeSH
- hojení ran MeSH
- hydroxyapatit * MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- náhodné rozdělení MeSH
- poranění páteře patologie radiografie terapie MeSH
- potkani Wistar MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury * MeSH
- transplantace kostí metody MeSH
- transplantace mezenchymálních kmenových buněk metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Reconstruction of a sport accident using detailed Finite Element (FE) model of the human head and explicit dynamics numerical simulation is presented in the paper. The sport accident involved a 13-year old boy on whom a handball cage fell during the school sport activity. FE model of the human head was developed using series of computer tomography (CT) scans. The FE model includes the skull, brain and subarachnoidal space. Rigid body model was used to assess initial conditions at the moment of the impact. The detailed FE model was imposed to the initial conditions obtained just before the head impacted the playground and differential equations of motion with explicit dynamics solver LS-DYNA (Livermore Software Dynamic Analysis) was used to determine the impact sequence. The pressure, shear stress response, von-Mises stress response and logarithmic strain values were evaluated in frontal, parietal, occipital and midbrain region. Head injury criteria were used to evaluate the injuries sustained. Results obtained from the numerical simulation of the accident showed good agreement with clinically observed head injuries and indicate the good ability of the FE model to simulate the impact situations and to investigate the brain injury mechanisms.
- MeSH
- biomechanika MeSH
- dítě MeSH
- kraniocerebrální traumata etiologie MeSH
- lidé MeSH
- počítačová simulace využití MeSH
- sportovní úrazy etiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- analýza metodou konečných prvků * využití MeSH
- lebka anatomie a histologie MeSH
- lidé MeSH
- magnetická rezonanční tomografie metody přístrojové vybavení využití MeSH
- modely anatomické * MeSH
- páteř anatomie a histologie MeSH
- počítačová rentgenová tomografie metody přístrojové vybavení využití MeSH
- projekty vizualizace člověka MeSH
- statistika jako téma MeSH
- teoretické modely * MeSH
- zobrazování dat MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
