Nanotubes with diameters ranging from 40 to 60nm were prepared by electrochemical oxidation of the Ti-6Al-4V alloy in electrolyte containing ammonium sulphate and ammonium fluoride. The nanotubes were further modified with calcium and phosphate ions or were heat treated. Polished Ti-6Al-4V alloy served as a reference sample. The spreading of human osteoblast-like cells was similar on all nanotube samples but lower than on polished samples. The number of initially adhered cells was higher on non-modified nanotubes, but the final cell number was the highest on Ca-enriched nanotubes and the lowest on heat-treated nanotubes. However, these differences were relatively small and less pronounced than the differences in the concentration of specific molecular markers of cell adhesion and differentiation, estimated by their intensity of immunofluorescence staining. The concentration of vinculin, i.e. a protein of focal adhesion plaques, was the lowest on nanotubes modified with calcium. Collagen I, an early marker of osteogenic cell differentiation, was also the lowest on samples modified with calcium and was highest on polished samples. Alkaline phosphatase, a middle marker of osteogenic differentiation, was observed in lowest concentration on nanotubes modified with phosphorus and the highest on heat-treated samples. Osteocalcin concentrations, a late marker of osteogenic cell differentiation, were similar on all tested samples, although they tended to be the highest on heat-treated samples. Thus, osteogenic differentiation can be modulated by various additional treatments of nanotube coatings on Ti-6Al-4V implants.

• MeSH
• buněčná adheze účinky léků   MeSH
• buněčná diferenciace účinky léků   MeSH
• fluorescenční mikroskopie MeSH
• kolagen typu I metabolismus   MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• nanotrubičky chemie   MeSH
• osteoblasty cytologie   metabolismus   MeSH
• osteokalcin metabolismus   MeSH
• titan chemie   farmakologie   MeSH
• vápník chemie   MeSH
• vinkulin chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

• MeSH
• aktiny metabolismus   MeSH
• biologické markery metabolismus   MeSH
• buněčná adheze účinky léků   MeSH
• buněčná diferenciace účinky léků   MeSH
• fluorescenční protilátková technika MeSH
• fotoelektronová spektroskopie MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• nanotrubičky chemie   MeSH
• osteoblasty cytologie   účinky léků   MeSH
• osteogeneze účinky léků   MeSH
• povrchové vlastnosti MeSH
• proliferace buněk účinky léků   MeSH
• titan farmakologie   MeSH
• velikost částic * MeSH
• viabilita buněk účinky léků   MeSH
• vinkulin metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Ti implants have become an important instrument in handling various problems associated with the loss of fle¬xibility of a joint system. Having replaced a damaged joint with an endoprosthesis reduces pain, restores flexibility and improves the patient's quality of life. The implant-bone integration may be a complicated and lengthy process. Therefore, the state of the material surface is of vital importance. Ti can be treated electrochemically to produce a tubular nanostructure, which can later be modified to achieve bioactivity. Moreover, the large adsorption surface enables anchoring of biologically active substances and pharmaceuticals. The nanostructure and its further modifications could facilitate and strengthen the implant-bone integration, and thus shorten the healing process. The article describes the preparation of nanostructures and summarizes the knowledge of Ti surface treatment.

• MeSH
• biokompatibilní materiály MeSH
• biomedicínský výzkum MeSH
• biotransformace MeSH
• chirurgie operační * MeSH
• integriny fyziologie   MeSH
• lidé MeSH
• molekuly buněčné adheze fyziologie   MeSH
• nanostruktury MeSH
• ortopedické výkony MeSH
• protézy a implantáty * MeSH
• titan * chemie   terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH