TiO2 along with nano-TiO2 are commonly found in consumer products. In vivo studies have observed an accumulation of nano-TiO2 in macrophages. However, characteristics of nano-TiO2 determining toxicity remain unclear. In our study, the cytotoxic effects of 14 diverse nano-TiO2 on THP-1 macrophage-like cells were measured by 3 cytotoxicity assays (MTS, WST-1 and LDH). Total averaged cytotoxicity was calculated using principal component analysis. Characteristics of all 14 nano-TiO2 included hydrodynamic diameter, zeta potential, shape, polydispersity index (PDI) and concentration; moreover, crystal form, specific surface area and crystallite size were measured for 10 nano-TiO2.The variables affecting cytotoxicity were chosen using LASSO (least absolute shrinkage and selection operator). Except for concentration, PDI in media measured within 1 h after preparation of the nanomaterial dispersion was selected as a variable affecting cytotoxicity: stable dispersion resulted in higher cytotoxic effects. Crystallite size has been shown to have nonlinear effects (particles of sizes between 20 and 60 nm were cytotoxic while smaller and larger ones were not) and thus it has been excluded from LASSO. The shape (particles/fibre) and crystal form did not affect the cytotoxicity. PDI and the nonlinear effect of size could be an explanation for the inconsistencies of the cytotoxicity of nano-TiO2 in various studies.
- MeSH
- endotoxiny analýza MeSH
- kultivační média MeSH
- lidé MeSH
- makrofágy účinky léků MeSH
- nanočástice chemie toxicita MeSH
- povrchové vlastnosti MeSH
- THP-1 buňky MeSH
- titan chemie toxicita MeSH
- velikost částic MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- FAKTOR VIII SEVAC,
- MeSH
- faktor VIII MeSH
- Haplorrhini MeSH
- hodnocení léčiv metody statistika a číselné údaje MeSH
- lidé MeSH
- spotřebitelská bezpečnost produktů MeSH
- stabilita léku metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- techniky in vitro MeSH
- Klíčová slova
- FAKTOR VIII SEVAC,
- MeSH
- faktor VIII aplikace a dávkování farmakokinetika terapeutické užití MeSH
- hemofilie A farmakoterapie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- FAKTOR VIII SEVAC,
- MeSH
- faktor VIII farmakokinetika krev MeSH
- hemofilie A farmakoterapie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- MeSH
- hemokoagulace MeSH
- lidé MeSH
- referenční hodnoty MeSH
- statistika jako téma metody MeSH
- Check Tag
- lidé MeSH
- MeSH
- antikoagulancia MeSH
- hodnocení léčiv MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- MeSH
- faktor VIII analýza nedostatek MeSH
- hemofilie A diagnóza MeSH
- vyšetření krevní srážlivosti MeSH
- Publikační typ
- srovnávací studie MeSH
