INTRODUCTION: The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. METHODS: Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. RESULTS: The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. DISCUSSION: The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.
- MeSH
- alfa-2-antiplasmin MeSH
- endoteliální buňky MeSH
- hojení ran MeSH
- lidé MeSH
- nanovlákna * MeSH
- obvazy MeSH
- polyestery farmakologie MeSH
- vaskulární endoteliální růstový faktor A * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- alfa-2-antiplasmin fyziologie MeSH
- fibrin-fibrinogen - produkty degradace diagnostické užití MeSH
- fibrinogen fyziologie MeSH
- fibrinolýza fyziologie MeSH
- hemokoagulace fyziologie MeSH
- invazivní růst nádoru MeSH
- látky modulující angiogenezi MeSH
- lidé MeSH
- tromboembolie patofyziologie MeSH
- Check Tag
- lidé MeSH
- MeSH
- alfa-2-antiplasmin analýza nedostatek MeSH
- dospělí MeSH
- fibrinolýza MeSH
- genetické nemoci vrozené patologie MeSH
- homozygot MeSH
- koagulopatie patologie MeSH
- krvácení genetika patologie MeSH
- lidé MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- alfa-2-antiplasmin nedostatek MeSH
- dospělí MeSH
- homozygot MeSH
- koagulopatie genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- alfa-2-antiplasmin analýza imunologie metabolismus MeSH
- dospělí MeSH
- farmakoterapie metody MeSH
- hemostáza účinky léků MeSH
- Hodgkinova nemoc farmakoterapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
