Objectives Dermatophytosis, commonly known as ringworm, is a superficial fungal skin disease and zoonosis. Pythium oligandrum is a micromycete with mycoparasitic properties that is used in agriculture to control fungal infections on plants. Formulations containing P oligandrum were also developed for the treatment of dermatophytoses, but only a small number of case studies have been published. In order to document the process in simplified conditions in vitro, we investigated the effectiveness of P oligandrum against three pathogenic dermatophytes common in domestic animals. Methods Cultures of the pathogens grown on nutrient media and experimentally infected cat hair were treated with P oligandrum preparations in therapeutic concentration and the changes were documented by microscopic videos and scanning electron microscopy. Results There was strong mycoparasitic activity of P oligandrum against Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes. Conclusions and relevance P oligandrum was demonstrated to be effective against three common causes of dermatophytosis in vitro.
- MeSH
- antifungální látky farmakologie MeSH
- kočky MeSH
- Microsporum účinky léků MeSH
- nemoci koček farmakoterapie mikrobiologie MeSH
- parazitické testy citlivosti veterinární MeSH
- Pythium * MeSH
- tinea farmakoterapie mikrobiologie veterinární MeSH
- Trichophyton účinky léků MeSH
- vlasy, chlupy mikrobiologie MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The composition of wax esters (WE) in the fur of adult greater mouse-eared bats (Myotis myotis), either healthy or suffering from white nose syndrome (WNS) caused by the psychrophilic fungus Pseudogymnoascus destructans, was investigated by high-resolution mass spectrometry analysis in the positive ion mode. Profiling of lipid classes showed that WE are the most abundant lipid class, followed by cholesterol esters, and other lipid classes, e.g., triacylglycerols and phospholipids. WE abundance in non-polar lipids was gender-related, being higher in males than in females; in individuals suffering from WNS, both male and female, it was higher than in healthy counterparts. WE were dominated by species containing 18:1 fatty acids. Fatty alcohols were fully saturated, dominated by species containing 24, 25, or 26 carbon atoms. Two WE species, 18:1/18:0 and 18:1/20:0, were more abundant in healthy bats than in infected ones.
- MeSH
- Chiroptera anatomie a histologie metabolismus mikrobiologie MeSH
- hmotnostní spektrometrie MeSH
- lipidy analýza MeSH
- mastné alkoholy analýza MeSH
- mastné kyseliny analýza MeSH
- mykózy mikrobiologie patologie veterinární MeSH
- nos mikrobiologie patologie MeSH
- sexuální faktory MeSH
- vlasy, chlupy chemie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
- MeSH
- antiperspiranty terapeutické užití MeSH
- axila MeSH
- barva vlasů MeSH
- Corynebacterium izolace a purifikace patogenita MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- financování organizované MeSH
- korynebakteriální infekce diagnóza mikrobiologie terapie MeSH
- lidé MeSH
- péče o kůži metody MeSH
- vlasy, chlupy mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- Bacteria MeSH
- dítě MeSH
- dospělí MeSH
- farynx mikrobiologie MeSH
- infekce spojené se zdravotní péčí etiologie prevence a kontrola MeSH
- lidé MeSH
- moč mikrobiologie MeSH
- novorozenec MeSH
- odběr biologického vzorku MeSH
- počet mikrobiálních kolonií MeSH
- vlasy, chlupy mikrobiologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
One-hundred and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees C. Using the hair-baiting technique with horse hair, 10 species of Chrysosporium were isolated: C. asperatum, C. state of Arthroderma tuberculatum, C. indicum, C. inops, C. keratinophilum, C. merdarium, C. pannorum, C. queenslandicum, C. tropicum and C. xerophilum. True dermatophytes were isolated: Trichophyton verrucosum and Trichophyton sp. Also, numerous fungi tolerating high levels of cycloheximide were encountered, such as members of Acremonium, Aspergillus and Penicillium. On plates of glucose or cellulose Czapek-Dox agar (free from sucrose) the most frequent fungi were: Alternaria alternata, Aspergillus flavus, A. flavus var. columnaris, A. fumigatus, A. niger, A. ochraceus, A. sydowii, A. terreus, Chaetomium globosum, Cladosporium herbarum, Emericella nidulans, Fusarium oxysporum, Mucor hiemalis, Penicillium chrysogenum, P. oxalicum, Scopulariopsis brevicaulis and Ulocladium atrum. On plates of 50% sucrose or 10 and 20% NaCl-Czapek's agar, some interesting species were frequently encountered: Eurotium amstelodami, E. chevalieri, E. halophilicum, E. montevidensis, E. repens, E. rubrum and Scopulariopsis halophilica. The isolated fungi have been tested for osmophilicity and halophilicity, they showed different rates of growth on sucrose and sodium chloride-Czapek's medium of various osmotic potential.
- MeSH
- chlorid sodný MeSH
- Chrysosporium růst a vývoj izolace a purifikace MeSH
- houby růst a vývoj izolace a purifikace MeSH
- keratiny MeSH
- koně MeSH
- kultivační média MeSH
- mikrobiologie vzduchu * MeSH
- osmóza MeSH
- prach * MeSH
- vlasy, chlupy mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Egypt MeSH
Twenty-six keratinolytic fungi (16 dermatophytes and 10 soil species) and sixteen non-keratinolytic fungi were cultivated on sterile human hair in a mineral solution. With keratinolytic fungi, the loss in total dry weight (hair + mycelium) reached 7.6 up to 24.2% after 60 days of cultivation. Peptidic (Lowry-positive) substances accumulated in the medium and pH of the medium gradually increased, often over pH 8. Excess of sulfur contained in the substrate was excreted back into the medium predominantly in the form of inorganic sulfate. Moreover, sulfite was produced that cleaved disulfide bonds of the substrate giving rise to S-sulfocysteine; soluble products of keratin degradation in the medium contained 2.1 to 6.5% of that substance. This supports the presumption that sulfitolysis of disulfide bonds is a key reaction of keratinolysis in fungi. Statistically significant correlations were observed between substrate degradation and medium alkalinization, as well as the contents of peptidic substances, sulfate, and S-sulfocysteine. The correlation was highest with sulfate content and least significant with peptidic substances. Non-keratinolytic fungi mostly grew on the hair but did not cause a gravimetrically measurable loss of the substrate, did not alkalinize the medium, and did not accumulate the above mentioned substances in amounts comparable with those of keratinolytic species.
- MeSH
- dítě MeSH
- houby izolace a purifikace metabolismus MeSH
- keratiny metabolismus MeSH
- lidé MeSH
- vlasy, chlupy mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- houby klasifikace MeSH
- lidé MeSH
- vlasy, chlupy mikrobiologie MeSH
- Check Tag
- lidé MeSH
